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Taxus chinensis extractive with blood sugar reduction effect, as well as preparation method and application thereof

A Taxus chinensis extract and hypoglycemic technology are applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, which can solve problems such as no diabetes drugs, and achieve convenient clinical application, strong activity, and clear ingredients. Effect

Inactive Publication Date: 2014-12-10
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Taxaceae (Taxaceae) Taxus genus ( Taxus.L ) plants including the southern yew [ T.mairei var.mairei (Lemcc et Level) cheng et L.K.Fu] and Tibetan yew ( T. wallichiana Zucc), Yunnan yew ( T. yunnanensis Cheng et.L.K.Fu), Chinese yew [ T. chinensis (Pilger) Rehd.], Northeastern yew ( T. cuspidata .Sieb.et.Zucc.) and Mandia yew ( T.media ), Taxus is widely used in the development of anti-tumor drugs, but there is no report on the development of drugs for the treatment of diabetes

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  • Taxus chinensis extractive with blood sugar reduction effect, as well as preparation method and application thereof
  • Taxus chinensis extractive with blood sugar reduction effect, as well as preparation method and application thereof
  • Taxus chinensis extractive with blood sugar reduction effect, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A yew extract with hypoglycemic effect, which is prepared by the following method:

[0037] 5 kg of branches and leaves of Taxus chinensis, cut into decoction pieces, extracted with 10 times the volume of 95% ethanol and 8 times 50% alcohol by reflux each time, combined the medicinal solution to concentrate the medicinal solution until it has no alcohol smell, and first extracted with petroleum ether to remove the pigment , and then extracted with ethyl acetate to obtain ethyl acetate extract; AB-8 type macroporous resin was applied to the ethyl acetate extract, eluted with distilled water and 0%-80% ethanol, and combined 60%-70% ethanol eluent Concentrate under reduced pressure and freeze-dry to obtain refined biflavonoids and phenolic acids.

[0038] Dissolve the effective part with 90% ethanol, and scan the sample at a wavelength of 350-600nm by ultraviolet spectrophotometry. There is maximum absorption at 504nm, and the measurement wavelength is determined to be 50...

Embodiment 2

[0040] A yew extract with hypoglycemic effect, which is prepared by the following method:

[0041]5 kg of branches and leaves of Taxus chinensis, cut into decoction pieces, extracted 3 times with 8 times the volume of 95% ethanol under reflux, combined the medicinal solution to concentrate the medicinal solution until it has no alcohol taste, first extracted with petroleum ether to remove the pigment, and then used ethyl acetate The ethyl acetate extract is extracted, and the ethyl acetate extract is separated by column chromatography on a polyamide column with a particle size of 15-60 mesh, and gradient elution is performed with ethanol with a concentration of 10-80%, to obtain refined biflavonoids and A mixture of phenolic acid compounds; the total flavone content measured by the method of Example 1 is 70%. The total flavonoids measured by HPLC contain five biflavones, which are Jinpine biflavanone, ginkgoxanthin, isoginkgoxanthin, ginkgo biflavanone, and spike flower biflav...

Embodiment 3

[0043] A yew extract with hypoglycemic effect, which is prepared by the following method:

[0044] 5 kg of southern yew branches and leaves, cut into decoction pieces, refluxed with 8 times the volume of 95% ethanol for three times, combined the liquid to concentrate the liquid until it has no alcohol taste, first extracted with petroleum ether to remove the pigment, and then with n-butanol Extract n-butanol extract. Put the n-butanol extract on the AB-8 type macroporous resin column, and carry out gradient elution with ethanol with a concentration of 50-80%, to obtain refined biflavonoids and glycoside mixtures; measure the total flavonoid content according to the method of Example 1 70%. The total flavonoids contained five biflavones and their glycosides were measured by HPLC, which were Jinpine biflavones and their glycosides, ginkgoxanthin and its glycosides, isoginkgoxanthin and its glycosides, ginkgo biflavones and their glycosides, and spike flower biflavones and its ...

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Abstract

The invention discloses a taxus chinensis extractive with blood sugar reduction effect. The taxus chinensis extractive is extracted from taxus chinensis plant branches and leaves by using modern separation methods. The content of refined biflavone in an effective part can be 50-80%; the main effective components are umbrella pine biflavone, ginkgetin, isoginkgetin, ginkgetin biflavone and amentoflavon. Experimental research shows that the taxus chinensis extractive can not only remarkably suppress the alpha-glucosidase activity, but also has an outstanding effect of reducing the rat blood sugar of diabetes, can relieve or suppress happening and development of diabetic complication, and has the hope to be developed into novel blood sugar reduction medicines.

Description

technical field [0001] The invention relates to a plant extract, in particular to an effective part and an active ingredient prepared from the branches and leaves of the southern yew and has the effect of lowering blood sugar. Background technique [0002] Today, diabetes has become the "third killer" that threatens human beings after cardiovascular and cerebrovascular diseases and tumors on a global scale. No matter in developed or developing countries, the incidence of diabetes is rising sharply. According to the data released by the World Health Organization, there were 30 million diabetic patients in the world in 1985, and it had increased to 135 million by 1997. According to the prediction of international authoritative diabetes epidemiology experts, by 2050, there will be 300 million people with diabetes in the world, and the growth rate of patients in Asia is very fast. . The disease not only brings great pain to the patients, but also greatly affects the quality of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K36/13A61K31/7048A61K31/192A61P3/10
Inventor 李祥陈建伟薛平汤彬姚晓
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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