Integrated microfluidic spinning chip and method for preparing regenerated silk fibroin by using same

Abstract

The invention belongs to the technical field of microfluidic spinning, relating to an integrated microfluidic spinning chip and a method for preparing regenerated silk fibroin by using the same. The integrated microfluidic spinning chip is of a three-layer structure, wherein the upper layer and the lower layer are provided with polydimethylsiloxane membranes of grooves, and the grooves are adhered with a middle dialysis membrane to form an upper microfluidic channel and a lower microfluidic channel. The depths of the two microfluidic channels are the same, and the widths gradually become small. The method for preparing the regenerated silk fibroin disclosed by the invention comprises the following steps: injecting spinning liquid in the upper channel and the lower channel at room temperature; injecting the mixed liquid of water-absorbent resin and ion adjusting liquid in the lower channel; and after the spinning liquid is adjusted via ions, cut, stretched and concentrated, extruding from an outlet, then solidifying in air as threads, and then winding the threads on rolls. The microfluidic chip disclosed by the invention can be used for simulating complex flow fields during the biological spinning process, and realizing the operations of the spanning liquid, such as cutting, stretching, component adjusting and concentrating. Compared with the spinning technology using a dry method, the method disclosed by the invention can be used for preparing good regenerated silk fibroin.

Description

technical field [0001] The invention belongs to the fields of bionics and microfluid spinning, and relates to an integrated microfluid spinning chip and a method for preparing regenerated silk fibroin fibers; in particular, it relates to a method for realizing shearing and pulling of spinning liquid in the same chip. A microfluidic dry spinning method for regenerated silk fibroin fibers integrating multiple functions such as stretching, component adjustment, concentration and spinning. Background technique [0002] Organisms such as spiders or silkworms can use water as a solvent at normal temperature and pressure to produce protein fibers with excellent mechanical properties. The entire process consumes low energy and does not use organic solvents, which is environmentally friendly. Therefore, the research on biomimetic spinning is of great significance to industrial fiber production. [0003] Although the comprehensive mechanical properties of spider silk are the best, th...

AI Technical Summary

Problems solved by technology

Patent No. ZL201110007232.6, designed a microfluidic chip with shear stretching function, combined with dry spinning, applied to the regenerated silk fibroin solution system, and realized the shear stretching of the spinning solution, but with Compared with the multiple functions of the biospinning process, the function is single and the degree of biomimesis is low

[0005] Although microfluidic technology has developed to a certain extent in the field of bionic spinning, compared with the biospinning process, the microfluidic spinning chip is still too simple and has a single function, and the cutting of the spinning solution has not been realized in the same chip. Cutting and stretching, component adjustment, concentration and spinning and other functions, there is still a big gap with the real bionic spinning

Images