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Triterpenoid saponin compound, extraction method of the triterpenoid saponin compound, and use of the triterpenoid saponin compound in treatment on myocardial ischemia/reperfusion injuries

A technology of triterpenoid saponins and extraction methods, which is applied in the direction of steroids, chemical instruments and methods, cardiovascular system diseases, etc., can solve the problems such as unclear active ingredients of Clematis clematis, and achieve strong activity and toxic side effects small effect

Inactive Publication Date: 2013-03-20
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Triterpenoid Saponins from Clematis tangutica. Planta Med, 2001, 67 (5), 484; Du ZZ, Zhu N.et al, Two new antifungal saponins from the Tibetan herbal medicine Clematis tangutica. Planta Med.2003,69:547-551), but the effective ingredients still unclear

Method used

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  • Triterpenoid saponin compound, extraction method of the triterpenoid saponin compound, and use of the triterpenoid saponin compound in treatment on myocardial ischemia/reperfusion injuries
  • Triterpenoid saponin compound, extraction method of the triterpenoid saponin compound, and use of the triterpenoid saponin compound in treatment on myocardial ischemia/reperfusion injuries
  • Triterpenoid saponin compound, extraction method of the triterpenoid saponin compound, and use of the triterpenoid saponin compound in treatment on myocardial ischemia/reperfusion injuries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Collect the whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province as a raw material. After the raw material is crushed into a coarse powder, take 3 kg, add 15 liters of ethanol with a concentration of 70% and carry out reflux extraction. Extract 3 times, each time 3 kg. Hour. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 540 g of ethanol extract. The extract was dispersed in 4.5 liters of water, and extracted 3 times with petroleum ether, 4.5 liters each time. The extracted aqueous phase was then extracted 3 times with n-butanol, 4.5 liters each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 120 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) and the gradient elution was chloroform-methanol-water m...

Embodiment 2

[0018] Collect the whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province as a raw material. After the raw material is crushed into coarse powder, take 3 kg, add 12 liters of ethanol with a concentration of 70% and carry out reflux extraction. Extract 3 times, each time 2 Hour. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 510 g of ethanol extract. The extract was dispersed in 3 liters of water, and extracted 3 times with petroleum ether, 3 liters each time. The extracted aqueous phase was extracted 3 times with n-butanol, 3 liters each time, the n-butanol extract was combined, and the solvent was recovered under reduced pressure to obtain 115 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) and the gradient elution was chloroform-methanol-water mixed solvent, and 1...

Embodiment 3

[0020] The whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province was collected as a raw material. After the raw material was crushed into a coarse powder, 1 kg was taken, and 3 liters of methanol were added for reflux extraction. A total of 3 extractions were performed for 1 hour each time. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 150 g of methanol extract. The extract was dispersed in 0.6 liters of water, and extracted 3 times with petroleum ether, 0.6 liters each time. The extracted aqueous phase was extracted 3 times with n-butanol, 0.6 liters each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 45 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) and the gradient elution was chloroform-methanol-water...

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Abstract

The invention discloses a triterpenoid saponin compound. The triterpenoid saponin compound is extracted from clematis tangutica and then is subjected to separation and purification. The triterpenoid saponin compound is shown in the formula (I) and comprises an aglycone of 3 beta, 23-dihydroxyolean-18-olefine acid. The triterpenoid saponin compound has obvious effects of protecting anoxic / oxygen-rich model-induced myocardial cell injuries, can be used for preparation of a drug for treating ischemic heart diseases, is a lead compound for researching and preparing a novel drug for treating ischemic heart diseases, and can provide a clinical drug which is used for treating myocardial ischemia / reperfusion injuries and has strong activity and small toxic and side effects.

Description

technical field [0001] The present invention relates to a new compound isolated from Clematis genus, in particular a triterpenoid saponin compound extracted and separated from Tibetan medicine Clematis clematis and its therapeutic effect on myocardial ischemia / reperfusion The application of the injury medicine belongs to the technical field of medicine. Background technique [0002] Ischemic heart disease is the main cause of death in humans, and its high morbidity and mortality seriously affect the quality of life of patients and are life-threatening. At present, the drugs for the clinical treatment of cardiovascular diseases mainly include nitrates, calcium antagonists and β receptor blockers, etc. Although these drugs have a certain curative effect, frequent toxic and side effects limit their application. [0003] Ranunculaceae Clematis clematis ( Clematis tangutica ), also known as Tangut clematis, and Tibetan medicine names are also called Yemangnabu and Yemeng. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P9/10A61P9/00
Inventor 李玉文文爱东奚苗苗张伟段佳林汤海峰
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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