Method for repeatedly extracting natural hirudin from live vampire leech

Abstract

The invention provides a method for repeatedly extracting natural hirudin from a blood-sucking leech living body, which comprises first extracting a natural hirudin crude product from a blood-sucking leech living body, and then obtaining natural hirudin powder through separation and purification. The extraction of the natural hirudin crude product is first Prepare a specific induction solution and pour it into the intestine to obtain the intestine containing the specific induction solution; then feed the intestine containing the specific induction solution to the blood-sucking leech, and then extract the saliva of the blood-sucking leech or grind the blood-sucking leech to obtain Slurry, that is, natural hirudin crude product is obtained; then conventional biochemical separation methods are used for separation and purification to obtain natural hirudin powder. The invention has the advantages of simple process, high extraction rate, and obviously reduces the death rate of natural hirudin crude product extracted by blood-sucking leeches, and can effectively improve the utilization rate of biological resources, thereby significantly reducing production costs, and is useful for extracting main effective active substances in leeches such as natural leeches It provides a convenient way.

Description

technical field [0001] The invention relates to a production method for extracting effective active ingredients from animal bodies, in particular to a method for producing natural hirudin by using blood-sucking leeches as raw materials. Background technique [0002] Leeches, commonly known as leeches, are the general term for leeches. It is a traditional precious Chinese medicinal material in my country. It has the functions of dispelling blood and promoting menstrual flow, eliminating accumulation and stasis, reducing swelling and detoxification. It is one of the 35 important traditional Chinese medicinal materials for promoting blood circulation and removing blood stasis in my country. [0003] According to their feeding habits, leeches can be divided into two categories: non-blood-sucking leeches and blood-sucking leeches: [0004] Non-blood-sucking leech, it mainly feeds on the juice of snails (such as snails, apple snails, etc.), mussels and other molluscs, and does no...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is: after the full-fed Hirudo phenanthae is artificially squeezed to make it secrete saliva, the oral cavity of Hirudo phenanthae is seriously damaged, which makes it easy to be infected with diseases or cause a large number of deaths due to its weakness and difficulty in recovery. At the same time, the time interval between the two extractions is too short, and Hirudo phenanthae cannot get nutritional supplements, and the body will not be fully recovered before entering the next extraction. In this way, on the one hand, the amount of crude hirudin extracted is small The content of hirudin is low, and more importantly, on the other hand, it will cause the death of Hirudo phenanthae to increase significantly, which will directly lead to high production costs

The disadvantage of this method is that the stimulating liquid contains neutral protease and papain, and these enzymes can destroy the chemical structure of hirudin, thereby reducing its activity and affecting the curative effect of hirudin.

The disadvantage of this method is: the use of chemical substances as the induction solution

The disadvantage of this method is that when a live leech is directly stimulated by a weak direct current, the leech will immediately shrink into a hard mass (because the leech is an invertebrate), and it is difficult to extract the hirudin

[0016] It can be seen from the above patents that chemical substances are used as the induction liquid to induce living blood-sucking leeches, and their safety has been questioned

At the same time, if the leech salivary gland secretion is taken out by artificial extrusion, that is, the natural hirudin crude product, the disadvantage is that there is no anti-infection drug in the induction solution, which will damage the oral cavity of the leech after extrusion, and directly cause the leech to be easily infected. Or frailty, decline in physical fitness, resulting in increased mortality, significantly increased production costs, and insignificant economic benefits