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Preparation method for tissue engineering collagen-hyaluronic acid-chondroitin sulfate electrostatic spinning bracket

A technology of chondroitin sulfate and hyaluronic acid, applied in the field of biomedical tissue engineering materials, can solve the problems of complex process, poor controllability, multiple catalysts, etc., and achieve the effect of uniform pore distribution, high porosity and simple equipment

Inactive Publication Date: 2013-03-06
川北医学院第二临床医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patented process is more complex, less controllable, and requires more catalysts

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Pre-prepared 2,2,2-trifluoroethanol and distilled water in a 1:1 volume ratio as solvents, take 80mg of collagen plus 1ml of solvent and soak overnight, then add 10mg of hyaluronic acid + 10mg of chondroitin sulfate to make a mixture with a total concentration of 10% , 100 rpm, stirring for 10 days is the electrospinning solution. The electrospinning solution is electrospinned in a high-voltage electrospinning device composed of a high-voltage power supply, solution storage device, injection device and receiving device. The electrospinning voltage is 20kv and the The example uses a 5ml glass syringe, a 6-gauge needle, a distance of 10cm from the needle to the receiving plate, a liquid injection speed of 10ml / h, and an ambient temperature of 30°C. After electrospinning, a collagen-hyaluronic acid-chondroitin sulfate three-dimensional electrospinning ternary nano-biological scaffold is obtained.

Embodiment 2

[0028] Pre-prepared 2,2,2-trifluoroethanol and distilled water in a 2:1 volume ratio as a solvent, 80mg of collagen plus 1ml of solvent soaked overnight, + 20mg of hyaluronic acid + 10mg of chondroitin sulfate to make a mixture with a total concentration of 11%. 500rpm, stirring for 3 days is the electrospinning solution. The electrospinning solution is electrospinned in a high-voltage electrospinning device. The electrospinning voltage is 30kv. This example uses a 5ml glass syringe, an 8 gauge needle, and the distance between the needle and the receiving plate is 15cm , The injection pumping speed is 15ml / h, and the ambient temperature is 10℃. After electrospinning, a collagen-hyaluronic acid-chondroitin sulfate three-dimensional electrospinning ternary nano-biological scaffold is obtained.

Embodiment 3

[0030] Pre-prepared 2,2,2-trifluoroethanol and distilled water in a volume ratio of 10:1 as solvents. After soaking in 1ml of solvent with 80mg of collagen overnight, +5mg of hyaluronic acid + 15mg of chondroitin sulfate to make a mixture with a total concentration of 10%. 3000rpm, stirring for 1 day is the electrospinning solution. The electrospinning solution is electrospinned in a high-voltage electrospinning device. The electrospinning voltage is 30kv. This example uses a 5ml glass syringe, a No. 5 needle, and the distance between the needle and the receiving plate is 5cm , The injection pumping speed is 5ml / h, and the ambient temperature is 20℃. After electrospinning, a collagen-hyaluronic acid-chondroitin sulfate three-dimensional electrospinning ternary nano-biological scaffold is obtained.

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PUM

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Abstract

The invention discloses a preparation method for a tissue engineering collagen-hyaluronic acid-chondroitin sulfate electrostatic spinning bracket, which is characterized by comprising the following steps: preparing a solvent, a glue solution, and the electrical spinning solution, and then performing electrostatic spinning. The bracket prepared by the method not only has a three-dimensional porous structure, but also has extracellular matrix highly bionic property with high tissue compatibility, controllable size and shape, and controllable porosity and diameter of spun nanofibers in a certain range; and the majority of extracellular matrix is a network comprising water, the collagen, polysaccharide (hyaluronic acid, chondrotin sulfate and the like), inorganic substance and the like, so that the collagen-hyaluronic acid-chondroitin sulfate electrostatic spinning bracket has a wide application prospect in the field of the tissue engineering bracket, in particular the soft tissue engineering bracket.

Description

Technical field [0001] The invention belongs to the field of biomedical tissue engineering materials, and is a preparation method of tissue engineering collagen-hyaluronic acid-chondroitin sulfate electrostatic spinning scaffold. Background technique [0002] In recent years, tissue engineering reconstruction has developed rapidly in the fields of cartilage, bone, skin, blood vessels and other fields and has made major achievements. There are also related products such as blood vessels, skin, and bone that have been used clinically. There are many types of scaffold materials as the adhesion and proliferation carrier of tissue engineering seed cells, and they are currently hot and important in the field of biomaterials. Scaffold materials provide cells with places for obtaining nutrients, gas exchange, excretion of waste, growth, differentiation and proliferation, and are also the material basis for tissue engineering to reconstruct tissues and organs. The ideal tissue engineerin...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/56D01F8/02D01F8/18D01D5/00
Inventor 冯刚杨泽龙陈竹刘康
Owner 川北医学院第二临床医学院
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