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Chaetomium globosum with nematicidal activity, metabolite and application thereof

A technology for chaetomium globosa and nematodes, applied in the direction of biocides, nematocides, biochemical equipment and methods, etc., to achieve low production costs, simple production process, and growth-promoting effects

Inactive Publication Date: 2014-05-14
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature search at home and abroad shows that there is no report on the secondary metabolites of Chaetomium globosa as nematode control agents

Method used

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  • Chaetomium globosum with nematicidal activity, metabolite and application thereof
  • Chaetomium globosum with nematicidal activity, metabolite and application thereof
  • Chaetomium globosum with nematicidal activity, metabolite and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. The culture characteristics of C. globosum NK106 of the present invention

[0022] The C. globosum NK106 strain of the present invention is isolated from the bark of pine (Cupressns sp.) in a certain place in the northeast. After the strain was cultured on the PDA plate at 28°C for 2 weeks, the bacterial body appeared brown (such as figure 1 A), collect the spores with sterile water to obtain a spore suspension. The spore suspension was transferred to a 500ml flask containing 200ml PDA potato medium, and cultured at 28°C and 180rpm shaking for 9 days (such as figure 1 Shown in B). Inoculate 1×10 per flask 5 Spores. PDA medium formula (g / L): 200g potato, 20g sucrose, 20g agar (added to solid medium).

Embodiment 2

[0023] Example 2. C. globosum NK106 fermentation product processing process provided by the present invention, product analysis and identification

[0024] The fermentation broth cultured in PDA in Example 1 was suction filtered to separate the hyphae from the fermentation broth; the fermentation supernatant obtained after suction filtration was mixed with an equal volume of dichloromethane and extracted overnight; a rotary evaporator was used After concentrating the organic phase filtrate obtained in the previous step, the crude product is obtained, which is analyzed, identified and collected by high performance liquid chromatography (HPLC).

[0025] HPLC selects analytical C-18ODS chromatographic column (4.6×250mm, Kromasil), chromatographic conditions: detection wavelength 227nm, flow rate 1mL / min, column temperature is room temperature, mobile phase is methanol:water=7:3. Experiments show that the fermentation product contains the same retention time as the ChA standard substan...

Embodiment 3

[0026] Example 3 Nematicidal activity of NK106 strain fermentation broth and purified ChA

[0027] The fermentation broth experiment has 5 treatments: 100%, 50%, 25%, 12.5% ​​fungal filtrate (Filtrates) and PDA medium (Medium, control). The ChA experiment has 3μg / mL, 30μg / mL, 300μg / mL and 4 treatments with 10% methanol (MeOH, control). Both treatments have sterile distilled water (SDW) as a blank control. Each treatment includes 6 repetitions. The following experimental methods (2) and (3) both use these two sets of treatments for experiments.

[0028] 1. Experimental method

[0029] (1) Preparation of nematodes and eggs

[0030] The eggs used in the experiment were obtained from the roots of Capsicum annuum L. infected by Meloidogyne incognita. After washing the soil on the roots, place it in 1% sodium hypochlorite and stir with a stirrer to release the eggs in the egg mass, and then pass the 200 mesh and 500 mesh sieve, and collect the eggs on the 500 mesh sieve. The second ins...

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Abstract

The invention relates to a chaetomium globosum with nematicidal activity, a metabolite and an application of the chaetomium globosum, belonging to the field of microbial pesticide technology. The preservation number of the chaetomium globosum NK106 is CGMCC6716. The invention includes the application of the chaetoglobosinA(ChA) generated by the bacterial strain in preventing the plant nematode. ChA has outstanding characteristics of high toxicity and remarkable nematicidal effect for Meloidogyne incognita. According to the invention, the effect of the strain fermentation broth and purified ChA on second-stage juveniles (J2) of Meloidogyne incognita is detected. The treatments of fermentation in different dilution concentrations have significant differences from the control treatment. The fermentation liquid and ChA has a lethal effect on the J2. When the concentration of the ChA is 300mug / mL, the lethality is 90.2%. Fermentation of all dilution concentrations inhibits the infection of the J2. After 30mg / kg ChA is added into the root of cucumber, the number of parasitized eggs is decreased by 63%, thereby showing that the ChA has a nematicidal activity and is biological nematicide with good application and development prospect.

Description

Technical field [0001] The invention belongs to the technical field of microbial pesticides. Specifically, it relates to a strain of Chaetomium globosum with nematicidal activity and a method for biologically controlling root knot nematodes using its fungal metabolites. Background technique [0002] The nematodes that seriously harm plants all over the world include root-knot nematodes, cyst nematodes, smooth blade nematodes, stem nematodes, grain nematodes and short-body (root rot) nematodes (Liu Weizhi, 2002). Among them, root knot nematodes (Meloidogyne spp.), as one of the important pathogens that harm crops, have caused great harm to large areas of crops around the world (Zhao Hong et al., 2003). [0003] The host range of root knot nematodes is very wide, with more than 3,000 species of plants belonging to 114 families. Plants of Solanaceae, Cucurbitaceae and Cruciferae are more common with root knot nematode disease. The life history of root knot nematodes is relatively s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 朱旭东胡阳毕建男阮维斌郝晓冉潘皎
Owner NANKAI UNIV
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