Chaetomium globosum with nematicidal activity, metabolite and application thereof

A technology for chaetomium globosa and nematodes, applied in the direction of biocides, nematocides, biochemical equipment and methods, etc., to achieve low production costs, simple production process, and growth-promoting effects

Inactive Publication Date: 2013-02-13
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature search at home and abroad shows that there is no report on the secondary metabolites of Chaetomium globosa as nematode control agents

Method used

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  • Chaetomium globosum with nematicidal activity, metabolite and application thereof
  • Chaetomium globosum with nematicidal activity, metabolite and application thereof
  • Chaetomium globosum with nematicidal activity, metabolite and application thereof

Examples

Experimental program
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Embodiment 1

[0021] Embodiment 1, the cultural characteristics of Chaetomium globosa C.globosum NK106 according to the present invention

[0022] The C. globosum NK106 strain of the present invention is isolated from the bark of pine and cypress (Cupressns sp.) somewhere in northeast China. After the strain was cultured on a PDA plate at 28°C for 2 weeks, the cells appeared brown (such as figure 1 A), the spores were collected with sterile water to obtain a spore suspension. The spore suspension was transferred to a 500ml flask containing 200ml PDA potato medium, and cultured with shaking at 180rpm for 9 days at 28°C (such as figure 1 shown in B). Each flask was inoculated with 1 x 10 5 spores. PDA medium formula (g / L): 200g potato, 20g sucrose, 20g agar (added to solid medium).

Embodiment 2

[0023] Example 2, the C.globosum NK106 fermentation product processing process provided by the present invention, the analysis and identification of the product

[0024] The fermented liquid cultivated in PDA in Example 1 is subjected to suction filtration, so that the mycelium is separated from the fermented liquid; the obtained fermentation supernatant after suction filtration is mixed with an equal volume of dichloromethane, extracted overnight; using a rotary evaporator Concentrate the organic phase filtrate obtained in the previous step to obtain a crude product, which is analyzed, identified and collected by high performance liquid chromatography (HPLC).

[0025] Analytical C-18ODS chromatographic column (4.6×250mm, Kromasil) was selected for HPLC, chromatographic conditions: detection wavelength 227nm, flow rate 1mL / min, column temperature is room temperature, mobile phase is methanol:water=7:3. It has been shown by experiments that the fermentation product contains sub...

Embodiment 3

[0026] The nematicide activity of embodiment 3, NK106 bacterial strain fermented liquid and purification ChA

[0027] There were 5 treatments of 100%, 50%, 25%, 12.5% ​​fungal filtrates (Filtrates) and PDA medium (Medium, control) in the fermentation broth experiment, and 3 μg / mL, 30 μg / mL, 300 μg / mL and 10% methanol (MeOH, control) 4 treatments. Both groups of treatments had sterile distilled water (SDW) as blank control. Each treatment consisted of 6 repetitions. The following experimental methods (2) and (3) were conducted using these two groups of treatments.

[0028] 1. Experimental method

[0029] (1) Preparation of nematodes and eggs

[0030]The eggs used in the experiment were obtained from the roots of colored peppers (Capsicum annuum L.) infested by Meloidogyne incognita. After washing the soil on the root, put it in 1% sodium hypochlorite and stir with a stirrer to release the eggs in the egg mass, then pass through 200 mesh and 500 mesh sieves, and collect the...

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Abstract

The invention relates to a chaetomium globosum with nematicidal activity, a metabolite and an application of the chaetomium globosum, belonging to the field of microbial pesticide technology. The preservation number of the chaetomium globosum NK106 is CGMCC6716. The invention includes the application of the chaetoglobosinA(ChA) generated by the bacterial strain in preventing the plant nematode. ChA has outstanding characteristics of high toxicity and remarkable nematicidal effect for Meloidogyne incognita. According to the invention, the effect of the strain fermentation broth and purified ChA on second-stage juveniles (J2) of Meloidogyne incognita is detected. The treatments of fermentation in different dilution concentrations have significant differences from the control treatment. The fermentation liquid and ChA has a lethal effect on the J2. When the concentration of the ChA is 300mug / mL, the lethality is 90.2%. Fermentation of all dilution concentrations inhibits the infection of the J2. After 30mg / kg ChA is added into the root of cucumber, the number of parasitized eggs is decreased by 63%, thereby showing that the ChA has a nematicidal activity and is biological nematicide with good application and development prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides. Specifically, it relates to a Chaetomium globosa strain with nematicidal activity and a method for biologically controlling root-knot nematodes by using the fungal metabolite thereof. Background technique [0002] The nematodes that seriously damage plants in the world include root-knot nematodes, cyst nematodes, slivers, stem nematodes, nematodes and short-bodied (root rot) nematodes (Liu Weizhi, 2002). Among them, root-knot nematode (Meloidogyne spp.), as one of the important pathogenic organisms that harm crops, has brought great harm to large areas of crops all over the world (Zhao et al., 2003). [0003] Root-knot nematodes have a wide host range, with more than 3000 species of plants belonging to 114 families. Plants such as Solanaceae, Cucurbitaceae, and Cruciferae are more common in root-knot nematodes. The life cycle of root-knot nematode is relatively simple. It develops...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 朱旭东胡阳毕建男阮维斌
Owner NANKAI UNIV
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