Bacillus cereus and application thereof
A Bacillus cereus, seed technology, applied in applications, bacteria, animal feed, etc., can solve the problems of water environment deterioration, chemical oxygen demand, biological oxygen demand exceeding the standard, incomplete purification, etc., to promote healthy development, The effect of enhancing disease resistance and enhancing immunity
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Embodiment 1
[0017] Screening and Identification of Example 1 Bacterial Strains
[0018] 1. Screening
[0019] (1) Take 50 mL of water samples from the aquaculture ponds of Shazikou aquaculture farmers in Laoshan District, Qingdao City, and bathe in 80°C water for 15 minutes to kill non-spore bacteria;
[0020] (2) After dilution, spread the water sample on nutrient agar medium (peptone 10.0g / L, beef extract powder 3.0g / L, sodium chloride 5.0g / L, agar 15.0g / L) plate, 37℃, Cultivate for 24 hours;
[0021] (3) Select typical Bacillus colonies to streak on the nutrient agar medium plate, culture at 37°C for 24 hours;
[0022] 2. Identification
[0023] Through the observation of strain morphology and the analysis of physical and chemical indicators, 20 strains were selected for the laboratory aquaculture wastewater treatment experiment. The experimental water body was 1000mL, and the ammonia nitrogen in the water before treatment was 0.966±0.017mg / L, and the nitrite nitrogen was 0.815±0.10...
Embodiment 2
[0031] The preparation of embodiment 2 bacillus cereus microbial preparation
[0032] 1. Preparation of slant strains: Streak inoculation of the above-mentioned Bacillus cereus on the nutrient agar medium, culture at 37°C for 12 hours;
[0033] 2. Preparation of solid seeds: Inoculate the slant strain prepared in step 1 to the fresh nutrient agar slant of the eggplant bottle, culture at 37°C for 12 hours;
[0034] 3. Preparation of liquid seeds: Wash the solid seeds prepared in step 2 from the slope of the eggplant bottle and inoculate them into the seed tank, culture at 37°C for 12 hours, the components of the medium in the seed tank and their weight ratios are: bean cake powder 2.5 %, corn steep liquor 2%, disodium hydrogen phosphate 0.1%, ammonium sulfate 0.05%, manganese sulfate 0.05%;
[0035] 4. Fermentation culture: inoculate the seeds in the seed tank into a 5-ton fermenter for cultivation, the inoculation amount is 5%, 37°C, 150rpm, and cultivate for 20h. The compone...
Embodiment 3
[0039] Application of embodiment 3 bacillus cereus microbial preparation
[0040] 1. The whole pool is splashed
[0041] After mixing the Bacillus cereus microbial preparation prepared in Example 2 with water, sprinkle the shrimp ponds in the whole pond. The dosage per mu (1 meter of water depth) is 0.5-1 kg. Sprinkle once every 10 days, continuously for 3 times, and detect the water body The content of ammonium nitrogen substance, nitrite nitrogen and COD in medium, concrete data are as shown in table 2:
[0042] Table 2 water quality parameters before and after treating shrimp ponds with SC2 preparation
[0043]
[0044] As can be seen from the table, after the Bacillus cereus of the present invention is processed, the degradation rates of ammonia nitrogen substances, nitrite nitrogen and COD in the water body of shrimp ponds reach 65%, 85% and 67% respectively, illustrating that the wax of the present invention Bacillus spp. SC2 can degrade ammonia nitrogen, nitrite ni...
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