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Biological activity factor composition for promoting ossification and vascularization

A technology of biologically active factors and transforming growth factors, which is applied in the direction of drug combinations, organic active ingredients, and medical preparations containing active ingredients, and can solve problems such as unfavorable practical clinical applications and long cycle times

Active Publication Date: 2013-01-30
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cell culture period in the early stage of the cell strategy is long, which is not conducive to actual clinical application

Method used

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  • Biological activity factor composition for promoting ossification and vascularization
  • Biological activity factor composition for promoting ossification and vascularization
  • Biological activity factor composition for promoting ossification and vascularization

Examples

Experimental program
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Effect test

preparation example Construction

[0068] The preparation method of the bioactive factor composition includes the step of adding the bioactive factor into the aqueous solution of sulfonated polysaccharide, and mixing uniformly to obtain the bioactive factor composition.

[0069] In another preferred example, the concentration of the sulfonated polysaccharide aqueous solution is 0.01-150 μg / mL, preferably, the concentration of the sulfonated polysaccharide aqueous solution is 0.02-100 μg / mL.

[0070] In another preferred example, the mass ratio of the bioactive factor to the sulfonated polysaccharide is 0.05-120:1, preferably, the mass ratio of the bioactive factor to the sulfonated polysaccharide is 0.1-100:1.

[0071] In another preferred example, the method further includes the step of freeze-drying the bioactive factor composition.

[0072] Freeze drying is also called sublimation drying. The drying method of freezing the water-containing material below the freezing point, turning the water into ice, and th...

Embodiment 1

[0092] Dissolve sulfonated chitosan with a molecular weight of 100,000 and a sulfur content of 8% in deionized water to prepare a 10 μg / mL sulfonated chitosan aqueous solution. Human bone morphogenetic protein-2 (BMP-2) is added according to the mass ratio of the bioactive factor to the sulfonated polysaccharide at 1:1, and mixed evenly to obtain the bioactive factor composition.

Embodiment 2

[0094] Dissolve sulfonated chitosan with a molecular weight of 300,000 and a sulfur content of 5% in deionized water to prepare a 50 μg / mL aqueous solution of sulfonated chitosan. Human bone morphogenetic protein-7 (BMP-7) is added according to the mass ratio of the bioactive factor to the sulfonated polysaccharide at 2:1, and mixed evenly to obtain the bioactive factor composition.

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Abstract

The invention discloses a biological activity factor composition for promoting ossification and vascularization, which comprises a biological active factor and carboxylated polysaccharide, the biological activity factor is one or more selected from a bone morphogenetic protein, a transforming growth factor, a blood vessel endothelial growth factor, a basic fibroblast growth factor and an ossification protein; and the carboxylated polysaccharide is one or more selected from sulfonated chitosan, sulfonated glucan, heparin, heparin sulfate and sulfate cellulose. The invention also discloses a preparation method of a composition and a transplant containing the composition. The composition provided by the invention can promote the ossification and the blood vessel generation, can be composited with a medically acceptable carrier, and can be used in the field of bone defect repair by employing an implantation mode or an injection mode.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a bioactive factor composition for promoting osteogenesis and angiogenesis, a preparation method and application thereof. Background technique [0002] The growth process of bone tissue is closely linked with vascularization. Sufficient blood supply is the decisive factor to ensure the activation of bone tissue engineering materials in vivo. During the embryogenesis of bone tissue or the healing process of bone injury, an excellent vascular network can provide good nutrient supply and material transportation for the tissue, and the lack of good blood supply will lead to abnormal bone growth and poor bone healing. In clinical bone grafting to repair defects, the bone graft bed is required to have a rich blood supply, and in autologous bone grafting, the blood supply is often enhanced by vascular pedicles or muscle flaps to promote the early survival of the grafted bone. Therefore, a relativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61K38/18A61P19/08A61K31/716A61K31/717A61K31/722A61K31/727A61K31/737
Inventor 刘昌胜曹玲燕王靖
Owner EAST CHINA UNIV OF SCI & TECH
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