Novel vascularized tissue-engineered bone and construction method thereof

Abstract

The invention relates to a novel vascularized tissue-engineered bone which comprises a cylinder support material, two internal axially-preset circular tunnels and arteriovenous blood vessel peduncles. The cylinder support material is a medical absorbable material, the two internal axially-preset circular tunnels are parallel to each other, an outlet circular chamfer is arranged at an outlet, and human or animal osteogenic induction stem cells are evenly distributed inside the circular tunnels. The arteriovenous blood vessel peduncles comprise arteries, veins and arteriovenous grafts, the arteries, the veins and the arteriovenous grafts are arranged on receptor blood vessel peduncles, the arteriovenous blood vessel peduncles are arranged in the support material and are arranged in parallel in a separated mode, and distal blood vessels are anastomosed or ligatured. According to the novel vascularized tissue-engineered bone, the tissue-engineered bone can be vascularized fast, fully, safely and effectively after transplantation, the clinical effects are effectively improved, good nutrition supply and the good metabolism environment are provided for transplanted cells, the osteogenic activity of the tissue-engineered bone after transplantation is promoted, and the wide clinical application of the grafts of the tissue-engineered bone is promoted.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a novel vascularized tissue engineering bone and a construction method thereof. Background technique [0002] The concept of tissue engineering (Tissue Engineering) was first proposed by Langer and Vacanti. According to the principles of cell biology and engineering, tissue cells with specific biological activities are combined with biomaterials to construct tissues and organs in vitro or in vivo. The science of maintaining, repairing, regenerating or improving the function of damaged tissues and organs. In the field of bone tissue engineering, the treatment of large bone defects with tissue engineered bone gradually shows attractive prospects, but it also faces some new problems. [0003] The vast majority of tissue cells in the living body are supplied with oxygen and nutrients by blood, limited by the diffusion of oxygen in the tissue, the oxygen supply range of capillaries ...

AI Technical Summary

Problems solved by technology

The vascular bundle presetting technology is based on the principle of axial blood supply in the marrow cavity of bone tissue. The distally ligated vascular bundle passes through the hole or groove in the central part of the tissue engineered bone. The vascular ingrowth and nutrition are from the center to the periphery. Lei Cai inoculated HA scaffolds with BMSCs from Beagle dogs, and repaired fibula bone defects in Beagle dogs with the help of vascularization with pre-prepared vascular bundles. Wang inoculated BMSCs with TCP scaffolds and pre-prepared saphenous arteriovenous bundles in rabbits to construct vascularized tissue engineering. bone and repair rabbit femoral bone defect, but because the above traditional vascular bundle prefabrication techniques all use the arteriovenous bundle to be placed in a separate hole or groove of a large-scale tissue engineering bone scaffold for prevascularization, the vascularized area is limited, The speed of vascularization is slow, and the promotion effect on tissue engineering bone osteogenesis is not ideal; the arteriovenous vascular loop preset technology (AV Loop) is a further improvement of the vascular bundle technology for these problems, inspired by Erol and Spira using arteriovenous Inspired by the rat model with axial vascular flaps constructed by loops, Kneser first constructed vascularized tissue-engineered bones by wrapping arteriovenous loops around tissue-engineered bones, and soon this technique has been widely used in small animals, Beier JP et al. used saphenous arteriovenous vascular loops to surround tissue-engineered bone in large animal models such as goats to construct vascularized tissue-engineered bone

Arteriovenous vascular loop technology has the advantages of blood vessel ingrowth and nutrition from the periphery to the center, and has the advantages of fast blood flow and strong angiogenesis effect. The length of the pedicle is high, the blood supply to the central part of the stent is limited, and it is not easy to operate during the transfer process, and it is easy to damage the blood vessel, so it is difficult to put into practical application

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