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Bacterial cellulose/collagen-chitosan composite material and preparation method thereof

A technology of bacterial cellulose and composite materials, applied in the fields of biomedical materials, antibacterial and cosmetic materials, can solve the problems of destroying the three-dimensional network structure of bacterial cellulose, reducing the mechanical properties of materials, and difficult to interpenetrate network structure, etc. Purity, excellent mechanical properties, the effect of enhancing mechanical properties

Active Publication Date: 2013-01-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difference in the microstructure of bacterial cellulose and collagen, it is difficult to ensure that the collagen can be immersed in the bacterial cellulose by direct impregnation, especially for short-term impregnation.
Although the solution blending-freezing-thawing method can prepare bacterial cellulose / collagen composite materials, this method destroys the original three-dimensional network structure of bacterial cellulose, and it is difficult for the two to form an interpenetrating network structure, thus greatly reducing the mechanical properties of the material. performance
In addition, although a certain bacterial cellulose / collagen composite material can be prepared by using fermentation in situ culture method, the production cycle is long, the cost is high, and the processing process is complicated.

Method used

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  • Bacterial cellulose/collagen-chitosan composite material and preparation method thereof
  • Bacterial cellulose/collagen-chitosan composite material and preparation method thereof
  • Bacterial cellulose/collagen-chitosan composite material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Step 1, preparation of bacterial cellulose

[0039] Medium composition: yeast powder 10g, peptone 6g, MgSO 4 2g, CaCl 2 0.1g, glucose 20g, coconut milk 1000ml, add 10ml absolute ethanol after sterilization.

[0040]Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 180rpm for 18 hours, then inoculate the fermentation medium at a ratio of 10%, and culture it statically for 7 days.

[0041] Step 2, purification of bacterial cellulose

[0042] Rinse the bacterial cellulose membrane prepared in step 1 with a large amount of tap water to remove the medium and impurities on the surface of the bacterial cellulose; then soak it in deionized water until it is colorless and transparent; soak it twice in 3wt.% SDS, and replace the SDS every 12 hours solution, and then soak 2 times with 1wt.% SDS, replace the SDS solution every 12h; soak 4 times with 0.1M sodium hydroxide solution, replace the sodium hydroxide solution once ...

Embodiment 2

[0054] Step 1, preparation of bacterial cellulose

[0055] Medium composition: yeast powder 10g, peptone 6g, MgSO 4 2g, CaCl 2 0.1g, glucose 20g, coconut extract 1000ml, add 10ml absolute ethanol after sterilization.

[0056] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 180rpm for 18 hours, then inoculate the fermentation medium at a ratio of 10%, and culture it statically for 7 days.

[0057] Step 2, purification of bacterial cellulose

[0058] Rinse the bacterial cellulose membrane prepared in step 1 with a large amount of tap water to remove the medium and impurities on the surface of the bacterial cellulose; then soak it in deionized water until it is colorless and transparent; soak it twice in 3wt.% SDS, and replace the SDS every 12 hours solution, and then soak 2 times with 1wt.% SDS, replace the SDS solution every 12h; soak 4 times with 0.1M sodium hydroxide solution, replace the sodium hydroxide solution ...

Embodiment 3

[0070] Step 1, preparation of bacterial cellulose

[0071] Medium composition: yeast powder 10g, peptone 6g, MgSO 4 2g, CaCl 2 0.1g, glucose 20g, coconut extract 1000ml, add 10ml absolute ethanol after sterilization.

[0072] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 180rpm for 18 hours, then inoculate the fermentation medium at a ratio of 10%, and culture it statically for 7 days.

[0073] Step 2, purification of bacterial cellulose

[0074] Rinse the bacterial cellulose membrane prepared in step 1 with a large amount of tap water to remove the medium and impurities on the surface of the bacterial cellulose; then soak it in deionized water until it is colorless and transparent; soak it twice in 3wt.% SDS, and replace the SDS every 12 hours solution, and then soak 2 times with 1wt.% SDS, replace the SDS solution every 12h; soak 4 times with 0.1M sodium hydroxide solution, replace the sodium hydroxide solution ...

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Abstract

The invention discloses a bacterial cellulose / collagen-chitosan composite material and a preparation method thereof. The method comprises the following steps of: 1) preparing bacterial cellulose and purifying the prepared bacterial cellulose; 2) fully immersing collagen into a bacterial cellulose network by a negative-pressure osmose process or a positive-pressure method, and crosslinking collagen in the bacterial cellulose / collagen composite material by using a cross-linking agent to obtain a bacterial cellulose / collagen interpenetration network composite material; and 3) removing the unreacted cross-linking agent from the composite material, and soaking in an antibacterial agent solution of chitosan or the derivative of chitosan to prepare the composite material with an antibacterial effect. The bacterial cellulose / collagen composite material is prepared by the negative-pressure method or the positive-pressure method, so that the defect of insufficient immersing amount of collagen in other preparation methods is overcome, and the problems of poor effect, long production cycle and high cost are solved; and the prepared composite material is excellent in mechanical property, high in water permeability, high in air permeability, high in biocompatibility, high in biological activity and high in antibacterial property.

Description

technical field [0001] The invention relates to a bacterial cellulose / collagen-chitosan composite material and a preparation method thereof, belonging to the fields of biomedical materials, antibacterial and cosmetic materials, and the like. Background technique [0002] Bacterial cellulose (BC for short) is a kind of natural high molecular polymer produced by microbial fermentation. Its chemical structure is the same as that of plant cellulose. However, the macrostructure of BC is quite different from that of plant cellulose, and has special structures and properties. BC has a natural fine network structure, high purity, high degree of polymerization and crystallinity, high tensile strength, strong water holding capacity, good water permeability and air permeability, good biocompatibility, biodegradability, and good controllability in the synthesis process . Based on its excellent properties, bacterial cellulose biomedical materials have broad application prospects and po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L1/02C08L89/00C08L5/08C08J3/24C12P19/04C12R1/02
Inventor 任力王迎军朱勇军李立风
Owner SOUTH CHINA UNIV OF TECH
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