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Cyanotis root extract rich in beta-ecdysone and preparation method thereof

A technology of ecdysone and dew grass, applied in botany equipment and methods, skin care preparations, medical preparations containing active ingredients, etc., can solve the problems of unsatisfactory content of β-ecdysone and ineffective drug effect, etc. To achieve the effect of ensuring product quality, wide application and maintaining biological activity

Active Publication Date: 2013-01-16
CHUXIONG BAICAOLING PHARMA DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a dew herb preparation on the market in my country. Due to the limitation of the extraction and separation technology of the existing technology, the content of β-ecdysone in the preparation is not ideal, resulting in insignificant efficacy.

Method used

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  • Cyanotis root extract rich in beta-ecdysone and preparation method thereof
  • Cyanotis root extract rich in beta-ecdysone and preparation method thereof
  • Cyanotis root extract rich in beta-ecdysone and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Choose 2-year-old dew grass without rot, mildew, and no visible impurities, and get 200 kg of dry roots and coarsely grind it to a 70-mesh sieve; Circulate extraction at 100°C for 1.8h, concentrate the filtrate under normal pressure, and dry the crude extract under microwave at 45°C to obtain the crude product; after crushing the crude product, add 6 times the weight ratio of ethyl acetate for extraction and separation, recrystallize and dry to obtain Dew grass extract 4kg. As determined by high performance liquid chromatography, the content of β-ecdysone reached 13.4%.

Embodiment 2

[0033] Select 2-year-old dew grass without rot, mildew, and no impurities visible to the naked eye, and get 200 kg of dry roots and coarsely grind it to pass through an 80-mesh sieve; Circulate extraction at 100°C for 2.2 hours, put the filtrate on a D101 macroporous resin adsorption column, control the flow rate at 90kg / h, elute with 90% ethanol 4 times the volume of the column, collect the eluate, recover the ethanol, and dry it in vacuum The crude product of the extract was obtained; the crude product was pulverized and then extracted and separated by adding ethyl acetate with a weight ratio of 6 times, and after recrystallization and drying, 1.8 kg of Dewwort extract was obtained. As determined by high performance liquid chromatography, the content of β-ecdysone reached 50%.

Embodiment 3

[0035] Select 1-year-old dew grass without rot, mildew, and no impurities visible to the naked eye, and get 200 kg of dry roots and coarsely grind it to pass through a 40-mesh sieve; Circulate extraction at 80°C for 1.5 hours, after the filtrate naturally precipitated for 12 hours, the supernatant was centrifuged with a high-speed centrifuge at a speed of 35,000r / min for 25 minutes, and the centrifuged liquid was placed on an AB-8 macroporous resin adsorption column, and the flow rate of the column was controlled at 80kg / h , eluted with 80% ethanol of 5 times the column volume, collected the eluate, recovered the ethanol and precipitated crystals; after recovering the ethanol, microwave drying at 50 ° C to obtain the crude extract; Ethyl acetate extracted and separated, recrystallized and dried to obtain 1.85kg of dew grass extract. As determined by high performance liquid chromatography, the content of β-ecdysone reached 53.1%.

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Abstract

The invention discloses cyanotis root extract rich in beta-ecdysone and a preparation method thereof. The cyanotis root extract comprises 50-90% of beta-ecdysone. The cyanotis root extract made from cyanotis root is prepared by water circulation extraction, centrifugal separation, resin absorption, and purification. The preparation method particularly includes: selecting annual or biennial cyanotis, removing impurities, taking dried root for grinding, adding 4-10 times of water for circulation extraction for 1.5-2.5 hours, performing centrifugal separation, applying macroporous resin absorption columns to liquor, eluting with ethanol, recycling elution crystallization, adding ethyl acetate for recrystalization, and drying to obtain cyanotis root extract. The cyanotis root extract made from annual or biennial cyanotis is rich in beta-ecdysone as raw material. The cyanotis is a biological product raw material having excellent properties, and is widely applicable to preparing and production of health-care products and pharmaceuticals. Beta-ecdysone content of the cyanotis root extract prepared by the method is increased greatly and can reach more than 90%. Biological activity of beta-ecdysone can be maintained effectively by low-temperature process control, and product quality is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of extraction of effective plant components, and specifically relates to a fast and efficient extract of dew grass rich in β-ecdysone and a preparation method thereof. Background technique [0002] Dew grass (Cyanotis arachnoidea CB.Clarke) is a perennial herb of the Commelinaceae blue ear grass. Aliases include Pearl Dew Grass (Qujing, Yunnan), Cockscomb Ginseng (Kunming, Yunnan), Anemarrhena (Mengzi, Yunnan), Spider Hair Blue Ear Grass, etc. The root of Lushuicao is used as medicine, which has the functions of dispelling wind and activating collaterals, promoting dampness and reducing swelling, and reducing asthenic fever. It is used to treat rheumatoid arthritis, numbness of limbs, low back and leg pain, nephritis edema, persistent fever and other symptoms. It can also be used externally to treat athlete's foot, eczema, knife wounds, etc. The stems and leaves are also good feed additives. In my country,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K8/97A01N65/08A01P7/04A61Q19/08A61P3/06A61P3/10A61P9/12A61K125/00
Inventor 曹志坚周绍光
Owner CHUXIONG BAICAOLING PHARMA DEV
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