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Production process of guanosine

A production process and technology of guanosine, applied in the field of fermentation production of guanosine, can solve the problems of complex production process and low yield of guanosine, etc.

Inactive Publication Date: 2013-01-09
STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two biosynthetic pathways of nucleotides, one is the total synthesis pathway using glucose and other carbon and nitrogen sources, starting from 5'-phosphoribose, and the other is the synthesis of purine bases through ribosylation and phosphorylation Synthetic salvage pathway, but existing guanosine yields are low and production is complex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1 (shake flask fermentation)

[0010] Activation of strains: Activation of strains is to insert the preserved strains into the slant culture medium to expand the culture. Generally, it needs 2-3 generations of activation process. The purpose of multiple transfer cultures is to obtain pure and strong cultures, that is, to obtain Vigorous cultures with adequate inoculum numbers. Because the storage conditions are often inconsistent with the culture conditions, activation is required to allow the strains to gradually adapt to the culture environment. In this case, the glycerin seeds stored at -30°C were quickly thawed to room temperature, 0.1ml was inoculated on the activated slant, cultured at a constant temperature of 34°C for 20-24h, and stored at 10°C as working seeds. When in use, take a working seed and reinvigorate it again, cultivate it as a slant seed according to the above conditions, and then insert the seed solution.

[0011] Seed culture: In order...

Embodiment 2

[0015] Embodiment 2 (5L tank fermentation)

[0016] Put the seed liquid into a 5L automatic control fermenter according to 10% inoculum amount (dissolved oxygen in the fermented liquid is automatically displayed. The temperature and pH value are automatically controlled), the liquid volume is 3L, the ventilation rate is 5L / min, the cultivation temperature is 36°C, and the rotation speed is 150rpm . The pH value was controlled to be 6.4±0.03 by adding ammonia solution automatically. When there is a lot of foam in the fermentation process, add a few drops of sterilized foam enemy manually or automatically through the defoaming pump to quickly defoam, add corn steep liquor hydrolyzate at 0h, stop at 45 hours, the total amount is 10%, and stop fermentation at 50h .

[0017] Fermentation tank medium ratio: MSG 1.0%, urea 0.1%, peptone 2.0%, yeast powder 2.0%, corn steep liquor 1.0%, potassium dihydrogen phosphate 0.03%, ammonium sulfate 0.01%, magnesium sulfate 0.01%, calcium car...

Embodiment 3

[0018] Embodiment 3 (50L tank fermentation)

[0019] On the basis that shake bottle fermentation and 5L tank fermentation are feasible, scale-up fermentation is carried out. Seed preparation process is identical with embodiment 2. Insert the seed liquid into a 50L automatic control fermenter (fermentation liquid DO, temperature, PH value, rotating speed automatic control) by 10% inoculum amount, the liquid filling volume is 30L, the ventilation rate is 8L / min, the cultivation temperature is 36°C, and the rotating speed is 200rpm. The set pH value is 6.4±0.1 to automatically control the feeding of ammonia solution. There are more foams in the late stage of fermentation. The sterilized foam enemy is automatically added through the defoaming pump for defoaming. After 0 hours, the corn steep liquor hydrolyzate is added, and it stops at 45 hours. The total amount is 10%, and the fermentation stops at about 50 hours.

[0020] Fermentation tank medium ratio: MSG 1.0%, urea 0.1%, pe...

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PUM

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Abstract

The invention discloses a production process of guanosine. The production process comprises the following steps: activating strains, culturing seeds, fermenting and extracting guanosine. The production process is characterized in that the strain-activating step is performed as follows: unfreezing glycerol strains which are conserved at - 30+ / -3 DEG C to reach the room temperature, sucking 0.1+ / -0.02ml of the strains to be inoculated on an activation slope and standing and culturing at 34+ / -4 DEG C for 20-24 hours to obtain working seeds. The process has the characteristics of low investment and high guanosine yield, and is simple to operate.

Description

technical field [0001] The invention relates to a fermentation production method of guanosine. Background technique [0002] Guanosine (guanosine) is a kind of purine nucleoside substances, molecular formula C10H13N5O5, relative molecular mass 283.24, white crystalline powder, dihydrate is needle crystal, dehydration at 110°C, melting point 240°C (decomposition), specific optical rotation Temperature -60°, soluble in dilute mineral acid, hot acetic acid, dilute alkali (1g / 33mL), boiling water, slightly soluble in cold water (1g / 1320mL), insoluble in alcohol, ether, chloroform and benzene. Guanosine is a multipurpose nucleoside. As a raw material for the synthesis of nucleoside antiviral drugs ribavirin, ascorguanosine and food freshener 5'-guanylic acid, its application prospect is no less than that of inosine . Guanosine is used as an intermediate of many high-efficiency drugs in the pharmaceutical industry. It can be used in the production of drugs such as ribavirin, asc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40
Inventor 黄永嫦谢畅丰郑凝坚冯今亮
Owner STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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