Method for solid liquid mixed culture of medical hericium erinaceus mycelia

A liquid mixing and solid medium technology, applied in the field of microbial cultivation, can solve the problems of short cycle, high efficiency, low mycelium production, uneven medium, etc., and achieve the effect of high immune activity

Active Publication Date: 2012-12-19
山西旺龙药业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly three kinds of production approaches of Hericium erinaceus: 1. Artificial cultivation, the advantage is that it is simple and easy to implement, and the cost is low; the disadvantage is that the cultivation period is long, about 3 months; 2, solid fermentation, cultivate Hericium erinaceus bacteria with solid medium Silk, to obtain secondary metabolites, its advantages are: the medium is simple and widely sourced, and the price is cheap; the fermentation process generally does not require strict aseptic operation; the oxygen supply and temperature during the cultivation process can be controlled by direct air forced ventilation; The treatment of fermentation residue is simple, and it can be directly used as feed or fertilizer; the disadvantages are: the water activity of the medium is low, the medium is uneven and difficult to stir, the growth of bacteria, the absorption of nutrients and the secretion of metabolites are uneven, making The monitoring and control of fermentation parameters is difficult, so continuous operation and automation are difficult; the temperature control of the heat generated by microbial respiration and metabolism is very difficult; the use of solid-state culture is mostly based on empirical data and operating experience, and it is easy to contaminate miscellaneous bacteria; 3. Liquid deep layer Fermentation to obtain the best operating conditions to produce a large amount of mycelium and metabolites in a short period of time. The advantages are: industrial continuous production can be carried out, a wide range of sources of culture raw materials, the best culture conditions can be achieved by controlling the fermentation device, and the cycle is short. Efficiency High; the disadvantage is that the production of mycelium is low, and it is difficult to form a large-scale application

Method used

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  • Method for solid liquid mixed culture of medical hericium erinaceus mycelia
  • Method for solid liquid mixed culture of medical hericium erinaceus mycelia
  • Method for solid liquid mixed culture of medical hericium erinaceus mycelia

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Embodiment 1

[0026] A method for solid-liquid mixed culture of medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,

[0027] (1). Preparation of solid medium

[0028]

[0029] Add the rest of the water, the pH is natural, mix evenly, and sterilize at 121°C for 30 minutes;

[0030] (2).Optimized screening of strains and isolation of fruiting bodies:

[0031] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;

[0032] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol for 2 minutes, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temperatur...

Embodiment 2

[0045] A method for solid-liquid mixed culture of medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,

[0046] (1). Preparation of solid medium

[0047]

[0048] Add the remaining amount of water, the pH value is 7.5, mix well, and sterilize at 118°C for 30 minutes;

[0049] (2).Optimized screening of strains and isolation of fruiting bodies:

[0050] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;

[0051] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol to disinfect for 1 minute, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep t...

Embodiment 3

[0064] A method for solid-liquid mixed culture of medicinal Hericium erinaceus mycelium, comprising the following steps: the percentages of the following substances are all percentages by weight,

[0065] (1). Preparation of solid medium

[0066]

[0067] Add the remaining amount of water, the pH value is 6, mix well, and sterilize at 121°C for 25 minutes;

[0068] (2).Optimized screening of strains and isolation of fruiting bodies:

[0069] Pick the fruiting bodies of wild Hericium erinaceus in the primeval forest of Zhongtiao Mountain, which are strong, well-developed, free from diseases and insect pests, and whose seeds are not scattered;

[0070] In the ultra-clean workbench above grade 100 in the inoculation room, immerse the fruiting body of Hericium erinaceus in 75% alcohol for 2 minutes, take it out, rinse it with distilled water, dry it with sterile gauze, and put the fruiting body on a petri dish , cover it with a bell jar or similar utensils, keep the room temp...

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Abstract

The invention discloses a method for solid liquid mixed culture of medical hericium erinaceus mycelia. According to the method, special medical white hericium erinaceus strains cultured from wild hericium erinaceus in Zhongtiao mountain primeval forest through optimization for many years are adopted. Compared with the ordinary edible hericium erinaceus, the mycelia produced from the strains have the advantages that polysaccharides and peptide are contained, five kinds of anticancer substances, seven kinds of vitamins, three kinds of active cellulase, 18 kinds of amino acids, human body necessary trace elements and the like are also contained, the contents of cystine, histidine and arginine with great medical values are high, the amino acids are identified for 6 to 7 points, and the protein content is 16.65 percent, and is respectively 3 times and 1.8 times that of other hericium erinaceus.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a method for cultivating medicinal Hericium erinaceus mycelium. Background technique [0002] Hericium erinaceus is named because its fruiting body resembles the head of a little monkey. It is a rare edible fungus with both edible and medicinal value. It is flat in nature and sweet in taste. Cancer, treatment of neurasthenia, for the treatment of digestive system diseases and tumors such as indigestion, gastric ulcer, esophageal cancer, gastric cancer, duodenal cancer, and spout cancer. At present, Hericium erinaceus has been successfully used in existing products, such as the marketed Hericium Jianweiling Capsules, in which Hericium erinaceus is the main ingredient of the product. [0003] At present, there are mainly three kinds of production approaches of Hericium erinaceus: 1. Artificial cultivation, the advantage is that it is simple and easy to implement,...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 史长山姜存敏潘志明王俊俊
Owner 山西旺龙药业集团有限公司
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