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One-step fermentation production process for low molecular weight dextran

A technology for dextran and dextranase, which is applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of high equipment cost, complicated production process, and high technical literacy requirements of production personnel, and achieves simple equipment, simplified technological process, and technical difficulty. small effect

Inactive Publication Date: 2012-12-12
GUANGZHOU SUGARCANE IND RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in China, sucrose is the main component of the culture medium, which is fermented by Leuconostoc enterococci to produce high molecular weight products. The fermentation broth is obtained by acid hydrolysis and ethanol fractional precipitation to obtain medicinal dextran products with different molecular weights. Not only the amount of ethanol is large , and there are disadvantages such as difficult process control and long settling time
The domestic research direction of the new process for the production of medicinal low molecular weight dextran is mainly the use of membrane technology for fractionation and separation. Although some progress has been made, the production process of this method is complicated, requires high technical literacy of production personnel, and high equipment costs. Therefore, the industrial scale Implementation is uneconomical

Method used

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  • One-step fermentation production process for low molecular weight dextran
  • One-step fermentation production process for low molecular weight dextran
  • One-step fermentation production process for low molecular weight dextran

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Experimental program
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Embodiment 1

[0033] The invention discloses a method for producing dextran by fermentation of Leuconostoc enterococcus.

[0034] 1) Activation of strains: activation of Leuconostoc enterica 20074 on a slant, cultured in an incubator at 28°C for 2 days;

[0035] 2) Primary seed culture: connect the Leuconostoc enterococcus grown on the slant to a 250mL Erlenmeyer flask with an inoculation loop, in which 100mL contains 0.14g of disodium hydrogen phosphate, 13g of sucrose, 0.2g of peptone, The liquid medium of potassium dihydrogen phosphate 0.03g was cultivated to OD under the conditions of rotation speed 100rpm and temperature 28°C 600 It is about 0.8 as a first-level seed;

[0036] 3) Secondary seed cultivation: put the first-stage seeds into the Erlenmeyer flask containing the above-mentioned liquid medium with 20% inoculum amount, and cultivate to OD at the speed of 100rpm and the temperature of 28°C. 600 0.8 or so is the secondary seed;

[0037] 4) Put the secondary seeds into a 5L au...

Embodiment 2

[0043] Method 1 for the one-step directed production of micromolecular dextran by enzymatic hydrolysis and cooperative fermentation.

[0044] 1) Strain activation: Leuconostoc enterococcus GSICC 31208 was activated on a slant, and cultured in an incubator at 28°C for 2 days;

[0045]2) Primary seed culture: connect the Leuconostoc enterococcus grown on the slant to a 250mL Erlenmeyer flask with an inoculation loop, in which 100mL contains 0.14g of disodium hydrogen phosphate, 13g of sucrose, 0.2g of peptone, The liquid medium of potassium dihydrogen phosphate 0.03g was cultivated to OD under the conditions of rotation speed 100rpm and temperature 28°C 600 It is about 0.8 as a first-level seed;

[0046] 3) Secondary seed cultivation: put the first-stage seeds into the Erlenmeyer flask containing the above-mentioned liquid medium with 20% inoculum amount, and cultivate to OD at the speed of 100rpm and the temperature of 28°C. 600 0.8 or so is the secondary seed;

[0047] 4) P...

Embodiment 3

[0051] Method 2 for the directional production of micromolecular dextran by enzymatic hydrolysis and cooperative fermentation one-step method.

[0052] 1) Activation of strains: activation of Leuconostoc enterica 20074 on a slant, cultured in an incubator at 28°C for 2 days;

[0053] 2) Primary seed culture: connect the Leuconostoc enterococcus grown on the slant to a 250mL Erlenmeyer flask with an inoculation loop, in which 100mL contains 0.14g of disodium hydrogen phosphate, 13g of sucrose, 0.2g of peptone, The liquid medium of potassium dihydrogen phosphate 0.03g was cultivated to OD under the conditions of rotation speed 100rpm and temperature 28°C 600 It is about 0.8 as a first-level seed;

[0054] 3) Secondary seed cultivation: put the first-stage seeds into the Erlenmeyer flask containing the above-mentioned liquid medium with 20% inoculum amount, and cultivate to OD at the speed of 100rpm and the temperature of 28°C. 600 0.8 or so is the secondary seed;

[0055] 4) ...

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Abstract

The present invention discloses a one-step fermentation production method for low molecular weight dextran. The production process comprises the following steps: inoculating Leuconostoc mesenteroides into a sucrose culture medium, and carrying out fermentation for 16-24 h to synthesize a fermentation liquid containing a dextran crude product; adding dextran enzyme to the fermentation liquid until a final dextran enzyme concentration is 0.05-5 U / mL, continuously carrying out fermentation, and carrying out the reaction for 2-8 h; after completing the reaction, carrying out inactivation to obtain a dextran crude product fermentation liquid; and carrying out a post-treatment on the crude product fermentation liquid to obtain the dextran finished product. Compared with traditional methods, the production process of the present invention has advantages of low technology difficulty, simple equipment, process simplification, and good control of low molecular weight dextran synthesis.

Description

technical field [0001] The invention relates to the technical fields of fermentation engineering and biocatalysis, in particular to a production method of low molecular weight dextran. Background technique [0002] Dextran (dextran) is a high molecular polymer polymerized by several glucose, also known as dextran, it can be produced by certain bacteria such as Leuconoto mesenteroides by fermenting sucrose, the mechanism is dextran sucrose secreted by bacteria The enzyme catalyzes the hydrolysis of sucrose into glucose and fructose, and polymerizes glucose to produce dextran. Dextran is the first microbial polysaccharide discovered and also the world's first industrialized production. [0003] The application of dextran in medicine is very common. For example, low-molecular-weight dextran is used as the main plasma substitute. This is the initial use of dextran after commercialization. There are three commonly used clinically: dextran-70, dextran-40, and dextran-20. , and t...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/08C12R1/01
Inventor 梁达奉蚁细苗阮恒吴兆鹏李雨虹曾练强许朝芳李锦荣韦少平
Owner GUANGZHOU SUGARCANE IND RES INST
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