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Bacillus amyloliquefaciens and its application

A technology of amylolytic spores and bacilli, applied in the direction of application, bacteria, and fungicides, can solve problems affecting product quality, health, harm to stems, pollution, etc., and achieve good development and application prospects, easy industrial production, and simple cultivation conditions Effect

Inactive Publication Date: 2012-12-12
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, strawberry gray mold (Botrytis cinerea Pers.ex Fr.) is the most serious harm. It harms stems, petioles, blades, and stipules during the annual growth period of strawberries, and harms flowers and fruits in the later stage, directly affecting strawberry yields, causing a lot of damage. big economic loss
The traditional control of the disease mostly uses chemical agents to spray the surface of the plants, resulting in varying degrees of pesticide residues and pollution in the soil, affecting product quality and people's health

Method used

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  • Bacillus amyloliquefaciens and its application
  • Bacillus amyloliquefaciens and its application
  • Bacillus amyloliquefaciens and its application

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0037] Experimental example 1: Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 on strawberry gray mold (Botrytis cinera Pers.ex Fr.), strawberry anthracnose (Colletotrichum fragariae A.N.Brooks), cocoa two spores [Lasiodiplodia theobromae (Pat.) Griffon & Maubl. ], Dothiorella gregaria Sacc., Phomopsis sp. and Apiocarpella macrospore (Speg.) H.&P.Syd].

[0038] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 strain activated with LB slant was streaked and inoculated on one side of the PDA plate, and then strawberry gray mold, anthracnose and 4 kinds of bacteria that caused poplar cankers (4 kinds of fungi that caused poplar cankers The germs are: Dispora cocoa cocciformis, polyspore shells, Phomopsis poplaris and Bacterobispora) cultures on PDA plates were punched with a puncher with a diameter of 5mm to get the same quality and the same amount of bacterial blocks, and inoculated on another plate. side. Cultivate at 28±1°C for 10 days to observe the ...

experiment example 2

[0041] Experimental example 2: Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 metabolites on strawberry anthracnose (Colletotrichum fragariae A.N.Brooks), strawberry gray mold (Botrytis cinera Pers.ex Fr.), cocoa two spores [Lasiodiplodia theobromae (Pat.) Griffon&Maubl.], Dothiorella gregaria Sacc., Phomopsis sp. and Apiocarpella macrospore (Speg.) H.&P.Syd].

[0042] The fermentation broth of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 cultured for 3 days was filtered with a bacterial filter, and the filter membrane pore size of the bacterial filter was 0.25 μm; the PDA plate culture of 6 kinds of plant pathogenic fungi was punched with a hole puncher with a diameter of 5 mm Take the same quality and the same amount of bacterial blocks and inoculate them on one side of the PDA plate; then punch the other side of the PDA plate with a 5mm diameter punch to get the same quality and the same amount of round blocks, and take out the round blocks; 10...

experiment example 3

[0045] Experimental example 3: A test to verify whether Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 has an inhibitory effect on strawberry gray mold.

[0046] 1. Experimental equipment: incubator, shaker, ultra-clean workbench;

[0047] 2. Experimental principle:

[0048] Before strawberry cultivation, put the strawberry roots in the culture solution of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293, take it out, drain it, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 can adhere to the strawberry roots to continue to grow and produce metabolic products, thereby inhibiting the growth of Botrytis cinerea.

[0049] 3. Experimental method:

[0050] 1) Cultivation of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 bacterial liquid: Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 into a Erlenmeyer flask containing 50 mL of LB medium, and culture at 35°C for 60 hours at 120 rpm.

[0051] 2) Preparati...

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Abstract

The invention discloses a bacillus amyloliquefaciens. Its classification name is (Bacillus amyloliquefaciens)B293. The bacillus amyloliquefaciens is preserved at the common microorganisms center of the CCCCM. Its collection number is CGMCC No.6081 and its collection date is April, 28th, 2012. The bacillus amyloliquefaciens is mainly used for biological control of strawberry botrytis. The bacillus amyloliquefaciens B293 has an effect of inhibiting a plurality of pathogenic fungi on strawberries and poplars. The antagonistic bacterium has a good effect of preventing and treating other plant diseases, is rapid to reproduce, can be artificially cultured, is easy to operate and convenient for production and application, has strong resistance ability, and especially is of great significance for preventing and treating strawberry botrytis caused by fungi. The antagonistic bacterium requires a simple culture condition, is easy to preserve, is easy for industrial production, and has a good development and application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B293 and its functions in preventing and treating strawberry botrytis, inhibiting the growth of strawberry anthracnose, inhibiting the growth of dispora cacao, inhibiting the growth of cocoon shells, inhibiting The growth of Phomopsis poplaris and its application in inhibiting the growth of Bacterobispora. Background technique [0002] In recent years, with the general concern of the public on the safety of chemical pesticides in food and the environment, and the prohibition of some dangerous chemical pesticides, people are seeking new ways to control plant diseases safely and effectively, and the use of beneficial microorganisms to control plant diseases has attracted increasing attention. It introduces exogenous antagonistic bacteria that are beneficial to plants into the broken plant natural ecosystem, or pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/07
Inventor 赵桂华徐超席刚俊史俊杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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