Biochemical culture and detection device as well as detection method thereof

Abstract

The invention relates to a biochemical culture and detection device as well as a detection method of the device, belonging to the technical field of microorganism culture. The biochemical culture and detection device comprises a bottom plate (1) and a top cover (3), wherein the bottom plate (1) comprises a culture pond (11) and a receiving region (12); bumps (122) in the receiving region are radially arranged around the culture pond (11) and used for dividing the receiving region (12) into multiple fan-shaped storage locations; culture sub-grooves (2) are like hollow wedge-shaped bodies and are arranged in the storage locations in the receiving region (12); the culture sub-grooves (2) adopt fully-opened culture sub-grooves (2a) or partially-opened culture sub-grooves (2b); the culture pond (11) is used for receiving liquid culture mediums to be detected; and the culture sub-grooves (2) are used for receiving detected culture mediums. The incubating process is finished by rocking the biochemical culture and detection device. The functions of enriched culture, coated incubation and biochemical identification are integrated, the operation is flexible, the requirement on operating environment and the detection cost are lowered, and the detection efficiency and the stability of results are improved.

Description

technical field [0001] The invention relates to a microorganism biochemical cultivation and detection device and a detection method thereof, belonging to the technical field of microorganism cultivation equipment. Background technique [0002] At present, in biomedical detection and research, bacterial detection occupies a large proportion. How to quickly and accurately identify the biological species of bacteria contained in the sample, the state of genetic variation, bacterial drug sensitivity, etc., has become an indispensable inspection item in daily work. [0003] The API biochemical detection kits currently on the market will microquantify and standardize the traditional methods that are cumbersome to operate and explain lengthy; combine the results of biochemical reactions with the results of other testing methods, compare them in the database that has been processed by statistical analysis, and then do Species discrimination. However, the API biochemical detection ...

AI Technical Summary

Problems solved by technology

[0004] 1. The API biochemical detection kit cannot realize the integration of bacterial enrichment culture and biochemical identification. It needs to carry out bacterial enrichment culture first, and then conduct a series of biochemical tests in the small tube containing the reagent

Due to the conversion of culture vessels and the design limitations of plastic tubes containing reagents, it is necessary to operate in a sterile environment, otherwise it may be contaminated by external bacteria, which will affect the test results.

[0005]2. The volume of the biochemical reaction test small tube is small, which brings inconvenience to the operation

And the medium containing certain nutrients is only suitable for the growth of some specific bacteria; if the bacteria contained in the sample to be tested is unknown, it is necessary to use multiple petri dishes to hold the medium containing different nutrients to try In order to meet the needs of isolating bacteria; in addition, it is necessary to carry out some special tests on the obtained single colony bacteria, such as Gram staining microscope, oxidase test, etc.; these must be met when using API kits The prerequisites increase the complexity and cumbersomeness of using the API system as a whole, and the whole process takes a long time

[0007] 4. API biochemical detection kits still use manual inoculation, which is inefficient and not suitable for large-scale operations

[0008] 5. The cost of using API biochemical detection kits is relatively high

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