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Epidemic encephalitis B/dengue chimeric virus with epidemic encephalitis B virus attenuated strain as gene framework and application of epidemic encephalitis B/dengue chimeric virus

A JE virus and gene technology, applied in the field of JE/dengue chimeric virus, can solve the problems of easy reverse mutation, poor attenuation effect, and low efficiency of attenuated strains, and achieve good application prospects, stable characteristics, High safety effect

Active Publication Date: 2012-11-28
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At first, live attenuated vaccines were prepared by subculture, but this method was inefficient and poorly attenuated, and the obtained attenuated strains were also prone to reverse mutations; especially the immune efficacy against the four serotypes of dengue virus was not balanced, so This attempt has been terminated

Method used

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  • Epidemic encephalitis B/dengue chimeric virus with epidemic encephalitis B virus attenuated strain as gene framework and application of epidemic encephalitis B/dengue chimeric virus
  • Epidemic encephalitis B/dengue chimeric virus with epidemic encephalitis B virus attenuated strain as gene framework and application of epidemic encephalitis B/dengue chimeric virus
  • Epidemic encephalitis B/dengue chimeric virus with epidemic encephalitis B virus attenuated strain as gene framework and application of epidemic encephalitis B/dengue chimeric virus

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Embodiment 1, constructing the Japanese encephalitis / dengue chimeric virus with the attenuated strain of Japanese encephalitis virus as the gene backbone

[0050] In this example, the Japanese encephalitis virus SA14-14-2 attenuated strain is used as the gene backbone to construct the JE / dengue chimeric virus, wherein the cDNA sequence corresponding to the genomic RNA of the Japanese encephalitis virus attenuated strain SA14-14-2 is as follows: As shown in Sequence 6 in the sequence listing, the 1st-95th position from the 5' end is the 5'UTR; the 96th-476th position is the coding gene (C) of capsid protein C; the 477th-2468th position is the deletion of the E protein The gene encoding the prM-E protein with 3 amino acid residues at the carboxy-terminus (prM-EΔ3); the 2469-2477th position is the encoding gene (E3) for the 3 amino acid residues at the carboxy-terminus of the E protein; the 2478th-10394th position It is the coding gene (NS) of non-structural protein NS; No...

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Abstract

The invention discloses a pidemic encephalitis B / dengue chimeric virus with an epidemic encephalitis B virus attenuated strain as a gene framework and application of the epidemic encephalitis B / dengue chimeric virus. The invention provides a deoxyribonucleic acid (DNA) molecule, and the DNA molecule is recombinant DNA which is obtained by replacing an encoding gene of protein A in complementary DNA (cDNA) corresponding to genome ribonucleic acid (RNA) of an epidemic encephalitis B virus by using an encoding gene of protein B; the protein A consists of an amino acid sequence shown as a sequence 1 in a sequence table; and the protein B consists of an amino acid sequence shown as a sequence 2 in the sequence table. The pidemic encephalitis B / dengue chimeric virus with the epidemic encephalitis B virus attenuated strain as the gene framework is a recombinant virus containing the DNA molecule. The pidemic encephalitis B / dengue type II chimeric virus also has a good application prospect when taken as a dengue vaccine and used for preventing dengue virus infection.

Description

technical field [0001] The invention relates to a Japanese encephalitis / dengue chimeric virus with an attenuated strain of the Japanese encephalitis virus as a gene skeleton and an application thereof. Background technique [0002] Dengue virus (DENV) belongs to Flaviviridae (Flaviviridae) Flavivirus genus (Flavivirus), this genus also includes epidemic Japanese encephalitis virus (referred to as Japanese encephalitis virus), also known as Japanese encephalitis virus (Japanese Encephalitis) virus, JEV), West Nile virus (WNV), tick-borne encephalitis virus (Tick-borne Encephalitis virus, TBEV) and yellow fever virus (Yellow Fever virus, YFV), etc., most of which are important The pathogens of zoonotic diseases are mainly transmitted by vectors such as mosquitoes and ticks. [0003] Dengue virus is divided into four serotypes, each of which can cause dengue fever (dengue fever, DF), dengue hemorrhagic fever (dengue hemorrhagic fever, DHF) and dengue shock syndrome (dengue sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/40C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N7/01A61K39/12A61P31/14
CPCY02A50/30
Inventor 秦成峰李玉华李晓峰于学东杨会强秦鄂德
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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