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Method for preparing powder of effective part of thunder god vine

A technology of effective parts and tripterygium wilfordii is applied in the field of preparation of powder of effective parts of tripterygium wilfordii, which can solve the problems of complex processing process, high production cost, weak pertinence and the like, and achieves simple operation, low production cost, and use of toxic organic solvents. less effect

Inactive Publication Date: 2012-11-28
MACAU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method of the active part powder of Tripterygium wilfordii, which solves the problems of poor pertinence, high production cost and complicated processing process of existing extraction methods.

Method used

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  • Method for preparing powder of effective part of thunder god vine

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Effect test

preparation example Construction

[0021] (1) Preparation of reference substance solution: Accurately weigh triptolide reference substance, ultrasonically dissolve it with ethanol with a concentration of 70%, and prepare a reference substance stock solution with a concentration of 0.15 mg / mL;

[0022] (2) Preparation of the test solution: take 30 mg of the active part of Tripterygium wilfordii, dissolve it with 5 mL of chloroform, and put it on the column (SPE small column, 6.0 cm long, 0.8 cm in diameter, 1.5 g of filler, 100-200 mesh oxidation Aluminum) was eluted with 20 mL of chloroform-methanol mixed solvent (volume ratio 95:5), the effluent and eluent were collected, evaporated to dryness under reduced pressure, ultrasonically dissolved with 70% ethanol, and the volume was adjusted to 25 mL;

[0023] (3) Linear relationship investigation: Accurately pipette 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL, and 0.8mL of the reference substance stock solution into graduated test tubes with stoppers, and add 70% etha...

Embodiment 1

[0046] The preparation method of tripterygium wilfordii effective part powder described in this embodiment comprises the following steps:

[0047] (1) Pulverizing step: select Tripterygium wilfordii bark rhizome 1.0kg, make tripterygium wilfordii powder after drying and pulverizing;

[0048] (2) Alcohol extraction step: the Tripterygium wilfordii powder is reflux extracted twice with 100% ethanol, after the two extracts are combined, the ethanol is recovered to obtain 70 g of crude extract;

[0049] (3) The first extraction step: the crude extract is extracted once with ethyl acetate solvent, the ratio of ethyl acetate solvent to crude extract is 8mL: 1g, and the extract is concentrated to 40% of the original volume to obtain concentrated extract;

[0050] (4) The second extraction step: the concentrated extract is extracted 3 times with a sodium carbonate aqueous solution with a concentration of 2wt%, and the volume ratio of the sodium carbonate aqueous solution and the conc...

Embodiment 2

[0052] The preparation method of tripterygium wilfordii effective part powder described in this embodiment comprises the following steps:

[0053] (1) Pulverizing step: select Tripterygium wilfordii bark rhizome 1.0kg, make tripterygium wilfordii powder after drying and pulverizing;

[0054] (2) Alcohol extraction step: the Tripterygium wilfordii powder is reflux extracted 3 times with ethanol with a concentration of 90wt%, after each extract is combined, ethanol is recovered to obtain 76g of crude extract;

[0055] (3) The first extraction step: extract the crude extract twice with ethyl acetate solvent, the ratio of ethyl acetate solvent to crude extract is 5mL: 1g, combine the extracts, concentrate the extract to the original 20% of the volume to obtain a concentrated extract;

[0056] (4) Second extraction step: extract the concentrated extract twice with 5wt% sodium carbonate aqueous solution, the volume ratio of sodium carbonate aqueous solution and concentrated extract...

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Abstract

The invention discloses a method for preparing powder of an effective part of thunder god vine, and belongs to a traditional Chinese medicine effective component extraction and separation technology. The method comprises the following steps of: extracting rhizome powder of the thunder god vine by alcohol; recycling a solvent; extracting extractum by an ethyl acetate solvent, and concentrating to a certain volume; extracting the ethyl acetate extracted solution by a carbonate aqueous solution with the concentration of 2 to 5 wt percent for 2 to 3 times, and recycling an organic layer; and drying to obtain the powder. The terpene content in the effective part powder is over 50 percent; and the capsanthin content, namely a toxic component, is less than 0.2 percent. The method is easy to operate, low in production cost, high in effective component content and low in toxicity and is mainly used for treating chronic nephritis diseases.

Description

technical field [0001] The invention belongs to a method for extracting and separating effective components of traditional Chinese medicines, and in particular relates to a method for preparing active part powder of Tripterygium wilfordii. technical background [0002] Relevant survey results show that the prevalence of chronic kidney disease in my country is about 8%. Various chronic kidney diseases, such as common chronic nephritis, chronic pyelonephritis, and autoimmune disease renal damage, have brought heavy burdens to patients' families and society. Chronic nephritis is a group of glomerular diseases mainly caused by chronic glomerular lesions with multiple etiologies. At present, it is believed that it may be caused by various bacterial, viral or protozoan infections, inflammatory mediator factors and non-immune mechanisms through immune mechanisms. Clinically, steroids and immunosuppressants are mainly used for treatment. Tripterygium Wilfordii (Tripterygium Wilfor...

Claims

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Application Information

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IPC IPC(8): A61K36/37A61P13/12A61K125/00
Inventor 林伟基杨祥良谢培山徐辉碧颜玉贞方伟彭帆张聪叶晓川
Owner MACAU UNIV OF SCI & TECH
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