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Traditional Chinese medicine extract capable of improving CIK cell proliferation rate as well as preparation method

A technology of cell proliferation and extracts, which is applied in the direction of drug combinations, pharmaceutical formulas, and medical preparations containing active ingredients, etc., can solve the problems of low cytotoxicity, difficult cell sources, and clinical application limitations, and achieve concise prescriptions, Clear ingredients, good social and economic benefits

Inactive Publication Date: 2013-10-16
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A key issue in tumor adoptive immunotherapy is to find suitable tumor-killing cells. Since the 1980s, cells including LAK, TIL, CD3AK, and CTL have been used in clinical practice, but due to the low expansion rate , difficult cell source, low cytotoxicity and many other problems, the clinical application of this therapy is limited

Method used

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  • Traditional Chinese medicine extract capable of improving CIK cell proliferation rate as well as preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0030] Example 1 A kind of formula and preparation method for preparing traditional Chinese medicine extract (SMH)

[0031] Take 5g of red ginseng, 5g of Ophiopogon japonicus, and 15g of astragalus. Heating purified water and decocting for extraction, the amount of water added is 10 times; decocting time is 0.5 hours. Collect the extract by filtration, concentrate the extract to obtain extract I; precipitate the above extract I with ethanol, filter, recover ethanol until it has no alcohol smell, and obtain refined extract II; enrich the refined extract II through a macroporous resin column , eluted with distilled water until colorless, eluted with ethanol, collected the eluate, concentrated, filtered through a 0.45 μm microporous membrane, and the filtrate was concentrated to 5 g crude drug / ml, boiled with activated carbon to absorb the heat source, removed carbon, and adjusted PH value, and filtered through a 0.22 μm microporous membrane to sterilize, subpackaged, and mad...

Embodiment 2

[0032] Example 2 Another formula and preparation method for preparing Chinese medicine extract (SMH)

[0033] Take 15g of red ginseng, 15g of Ophiopogon japonicus, and 30g of astragalus. Heating purified water and decocting for extraction, the amount of water added is 15 times respectively; the decocting time is 1 hour respectively. Collect the extract by filtration, concentrate the extract to obtain an extract; enrich the above extract through a macroporous resin column, elute with distilled water until it is colorless, elute with ethanol, collect the eluate, concentrate, and pass through a 0.45 μm micro Filtrate through a pore filter, concentrate the filtrate to 5g crude drug / ml, boil with activated carbon to absorb the heat source, remove carbon, adjust the pH value, filter through a 0.22μm microporous membrane to sterilize, sub-package, and make SMH Chinese medicine extract.

Embodiment 3

[0034] Example 3 A comparative experiment on the tumor cell killing ability of CIK cell culture added with traditional Chinese medicine extract (SMH) and conventional CIK cell culture after induction of proliferation and proliferation

[0035] (1) Co-stimulation culture with Chinese herbal extracts (SMH) and various cytokines

[0036] 30 mL of venous blood was collected in a vacuum blood collection tube containing anticoagulant, and peripheral blood mononuclear cells (PBMC) were separated with lymphocyte separation medium, washed twice, and then RPMI 1640 complete culture medium (containing inactivated 10% autologous plasma , 25 mmol / L HEPES, 100 U / ml penicillin, 100 U / ml streptomycin) to resuspend the cells and adjust the cell concentration to 2 × 10 6 / ml. The cells were equally divided into two groups, and the first group was cultured according to the conventional CIK cell culture method, that is, IFN-γ (1 000 U / ml) was added on the 0th day, and CO 2 After incubation in...

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Abstract

The invention relates to a traditional Chinese medicine extract capable of obviously improving CIK (Cytokine-Induced Killer) cell proliferation rate. The extract is prepared from the raw materials of red ginseng, radix ophiopogonis and milk vetch according to a certain weight ratio. A formula of the extract comprises the steps as follows: the red ginseng, the radix ophiopogonis and the milk vetch are added with water and decocted, and are subjected to ethanol precipitation and filtered; ethanol is recovered; the extract liquid is enriched with macroporous resin columns, eluted to be colorless with distilled water and eluted with ethanol; the eluant is collected; the extract liquid is filtered with a microporous filtration film; and the filter liquor is condensed, the pH value is adjusted, the filter liquor is filtered again with the microporous filtration film, sterilized, and subpackaged, and then the traditional Chinese medicine extract is obtained. Pharmacological experiments show that the traditional Chinese medicine extract can remarkably improve the proliferation rate of CIK cells.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a formula of a traditional Chinese medicine extract capable of increasing the proliferation rate of CIK cells and a preparation method thereof. Background technique [0002] Tumor biotherapy is a hot spot in the field of tumor research today. Tumor biotherapy includes cytokines, gene therapy, monoclonal antibodies, tumor vaccines and adoptive immunotherapy, among which tumor cell adoptive immunotherapy occupies an important role in tumor biotherapy. important position. Cell adoptive immunotherapy is a biological treatment method that has been studied more and has entered clinical trials. It is to infuse autologous or allogeneic immune effector cells activated in vitro into patients to kill tumor cells in patients; it is suitable for patients with low cellular immune function, such as high-dose chemotherapy, radiotherapy, bone marrow transplantation, and viru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8968A61P35/00A61P37/04
Inventor 陈春徐军
Owner CHINA JILIANG UNIV
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