Method for detecting fatty acid content by gas chromatography-mass spectrometry (GC-MS)

Abstract

The invention provides a method for detecting fatty acid content in animal sample by gas chromatography-mass spectrometry (GC-MS), to solve the problems of complicated sample treatment steps and high cost in conventional methods in practical production and scientific research. The method comprises the steps of treating animal tissue samples; extracting, saponifying and methylating fatty acids; determining GC-MS conditions for fatty acid detection; analyzing data and identifying sample. The method has the advantages of simple operation, low detection limit, good reproducibility, and visual andreliable experiment data; is especially suitable for trace sample detection, can compare fatty acid compositions and contents among different samples, has wider application, and provides a new thought and method for rapid detection of fatty acid composition and content in animal sample for scientific research, clinical application, and food quality and safety monitoring.

Description

technical field [0001] The invention relates to a method for rapidly detecting fatty acid content in animal samples based on gas chromatography-mass spectrometry (GC-MS method). Background technique [0002] Fatty acids are the basic substances that make up human fat and lipids, and are important components of cell membrane phospholipids, which directly regulate the composition of cell membranes, proteins and intramembrane receptors. Fatty acids can be divided into saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) according to the degree of saturation. [0003] Short-chain SFA can regulate intestinal motility, promote colon cell proliferation, increase circulating blood flow at intestinal mucosal injury, and promote the recovery of intestinal mucosal structure and function; in addition, short-chain saturated fatty acids can treat diversion colitis , distal ulcerative colitis and radiation colitis. In addition, excessive...

AI Technical Summary

Problems solved by technology

[0007] The determination of free fatty acids in plasma is to first combine fatty acids with Cu 2+ function, after extraction with chloroform, copper reagent was used to determine Cu 2+ concentration, and then get the content of free fatty acid, this method is not only cumbersome, but also can only measure the total amount of fatty acid

Although gas chromatography has a high separation ability, it must be compared with standard substances when qualitatively identifying fatty acids, and it is difficult to measure without standard samples

In addition, fatty acid components are complex and difficult to separate. Traditional methods usually use acid hydrolysis, alkali hydrolysis, and saponification.

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