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Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof

A quality inspection method and technology of Tibetan medicine, applied in measuring devices, chemical analysis by titration, instruments, etc., can solve the problems that it is difficult to ensure the safety, effectiveness, and health risks of medicinal materials

Active Publication Date: 2012-10-17
SHANDONG JINHE DRUG RES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The whole herb of Aconitum tangutica is used as medicine. The height of the herb is 10-30cm, the tuber is 2, and small in shape. It can be used as medicine after concocting, but overdose may bring health risks
Tangut Aconitum is recorded in the "Standard issued by the Ministry of Tibetan Medicine". The name of Tibetan medicine is Bangga. The quality of its medicinal materials is difficult to guarantee the safety and effectiveness of medicinal materials, so it is necessary to establish a scientific quality detection method to evaluate the quality of Aconitum tanguta and its preparations

Method used

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  • Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof
  • Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof
  • Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0054] Experimental example 1: TLC identification experiment of Tangutia aconitum

[0055] Tangut Aconitum, provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0056] TLC identification:

[0057] Take 1g of the medicinal material powder to be tested (passed through a No. 3 sieve), add 5ml of ammonia water, add 50ml of dichloromethane, sonicate (power 250W, frequency 40kHz) for 30 minutes, filter, evaporate the filtrate to dryness, and add 1ml of methanol to the residue to dissolve, as The test solution. Take 1g of the reference medicinal material of Aconitum tangutatus, and make a solution of the reference medicinal material in the same way. According to the test of thin-layer chromatography (Appendix VI B of the 2010 edition of the Pharmacopoeia of the People's Republic of China), draw 10 μl of each of the above two solutions, and place them on the same silica gel G thin-layer plate respectively, with a volume-to-part ratio of 5:4: 1.5: 0.2 n-hexane-ethyl...

experiment example 2

[0059] Experimental example 2: Examination of diester alkaloids in Aconitum tangutata

[0060] 1. Instruments, reagents and test samples

[0061] Instrument: High performance liquid chromatography: Hitachi L-2100 pump, Hitachi L-2400 UV detector;

[0062] Electronic balance: Shimadzu AUW220D type.

[0063] Reference substance: aconitine reference substance (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 110720-200410; hypoaconitine (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 110798-200805; Product Testing Institute), batch number: 110799-200505.

[0064] Sample: Tangut Aconitum (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers: 110701, 110702, 110703.

[0065] 2. Selection of detection wavelength

[0066] Take the mixed solution of aconitine reference substance, hypoaconitine reference substance and neoaconitine reference substance, and scan in the wavelength...

experiment example 3

[0123] Experimental Example 3: Determination of Hordenine Content in Tangut Aconitum

[0124] 1. Instruments, reagents and test samples

[0125]Instrument: High performance liquid chromatography: Hitachi L-2100 pump, Hitachi L-2400 UV detector;

[0126] Electronic balance: Shimadzu AUW220D type.

[0127] Reference substance: hordenine reference substance (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 100762-200901.

[0128] Sample: Tangut Aconitum (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers: 110701, 110702, 110703.

[0129] 2. Selection of detection wavelength

[0130] Take hordenine reference substance solution, scan in the wavelength range of 190-400nm, and select 225nm as the detection wavelength according to the ultraviolet absorption spectrum.

[0131] 3. Mobile phase and column selection

[0132] The acetonitrile-volume ratio is 0.5% triethylamine aqueous solution (phosphoric acid adjusts pH 3....

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Abstract

The invention discloses a method for detecting quality of alkaloids in a Tibetan medicine herba aconiti tangutici and preparations thereof. The method comprises the steps of identification, check and content determination, wherein thin layer chromatography is adopted for identification and is characterized by taking a herba aconiti tangutici control medicine as the control and normal hexane-ethyl acetate-methanol-ammonia water as a developer; high performance liquid chromatography is adopted for check and is characterized by taking a mixed solution of an aconitine control substance, a hypaconitine control substance and a new aconitine control substance as the control, acetonitrile-tetrahydrofuran as a mobile phase A and an ammonium acetate water solution with glacial acetic acid as a mobile phase B to carry out gradient elution; high performance liquid chromatography is adopted for hordenine content determination and is characterized by taking a hordenine control substance as the control and an acetonitrile-potassium dihydrogen phosphate water solution as a mobile phase; and back titration is adopted for total alkaloid content determination. The method is stable and reliable, has strong specificity, can ensure the safety, effectiveness and quality controllability of the alkaloids in the Tibetan medicine herba aconiti tangutici and the preparations thereof, and has higher scientificity and application values.

Description

technical field [0001] The invention relates to a quality detection method, in particular to a quality detection method of alkaloids in Tibetan medicine Tanguta aconitum and its preparation. The method includes the quality detection method of alkaloids in Tangut Aconitum and its preparations in Bawei Swertia Pills, alkaloids in Shishiwei Jinjin Pills, Shisanwei Pangga Powder, and Anerning Granules . Background technique [0002] Herba Aconiti Tangutici (Herba Aconiti Tangutici) is the dried whole plant of Aconitum tanguticum (Maxim.) Stapf and Aconitum naviculare (Bruhl.) Stapf of Ranunculaceae plants. During the flowering period in late summer and early autumn, the roots are excavated, impurities are removed, and dried in the shade. This product is cool in nature, bitter in taste, and has the effects of clearing away heat and detoxification, promoting granulation and closing the mouth, and drying dampness. Functions and indications Clearing heat and detoxifying, mainly u...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02G01N31/16G01N30/36G01N30/88
Inventor 邵成雷王海苹李丽任松鹏单玉刚刘玉芹郑亭亭
Owner SHANDONG JINHE DRUG RES DEV
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