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Molecular identification method of authenticity and category of rhinoceros horn products

A technology for rhino horn and horn products, which is applied in the field of molecular identification of the authenticity of rhino horn products and the type to which they belong, can solve problems such as the difficulty in distinguishing structural features, and achieve the effect of scientific and effective identification basis.

Inactive Publication Date: 2012-10-17
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

[0005] Traditional rhino horn identification methods are mainly morphological and histological identification. These methods can identify rhino horns with complete morphology. However, most of the rhino horns sold in the market are semi-finished products, powder or handicrafts after grinding, polishing, and coloring. Its structural features are not easy to distinguish. At this time, morphological observation may not be able to accurately determine the authenticity of rhino horn products or whether the products contain rhino horn components.

Method used

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  • Molecular identification method of authenticity and category of rhinoceros horn products
  • Molecular identification method of authenticity and category of rhinoceros horn products
  • Molecular identification method of authenticity and category of rhinoceros horn products

Examples

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Embodiment 1

[0030] Example 1. Identifying the authenticity of commercially available rhino horn products and which rhinoceros the rhino horn material is derived from

[0031] Sample 1 and sample 2 are two commercially available rhino horn products (rhino horn cups), of which sample 1 has been identified as a rhino horn product of the African black rhino, and sample 2 has been identified as a yellow cattle horn product (that is, a counterfeit rhino horn product ), perform the following operations on sample 1 and sample 2 respectively:

[0032] 1. Use an electric drill to drill a small amount of debris, and use the magnetic bead method (DNA Purification System for food, kit from Promega, USA) to extract the genomic DNA of each sample.

[0033] 2. Using each genomic DNA extracted in step 1 as a template, perform PCR amplification with a primer pair composed of F1 and R1 (the target gene is the COI gene) to obtain PCR amplification products. The agarose gel electrophoresis of the PCR amplifi...

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Abstract

The invention discloses a molecular identification method of authenticity and category of rhinoceros horn products and provides application of a universal primer pair in identifying which rhinoceros the rhinoceros horn materials in the rhinoceros horn products come from in an aided manner. The universal primer pair is formed by a DNA (deoxyribonucleic acid) molecule shown in Sequence 1 in a sequence table and a DNA molecule shown in Sequence 2 in the sequence table. The invention provides an accurate and reliable molecular biology technical method for identification of authenticity of the rhinoceros horn products, and provides scientific and effective identification bases for biological resource entry-exit inspection and species control, illegal trading prevention and port law enforcement.

Description

technical field [0001] The invention relates to a molecular identification method for the authenticity of a rhino horn product and its category. Background technique [0002] Rhinoceros in the world mainly includes five species, namely black rhinoceros (Diceros bicornis) and white rhinoceros (Ceratotherium simum) in Africa, Indian rhinoceros (Rhinoceros unicornis ), Javan rhinoceros (Rhinoceros sondaicus ) and Sumatran rhinoceros (Dicerorhinus ) in Asia. sumatrensis). [0003] Because rhino horns are used as valuable raw materials for Chinese patent medicines, rhinos have become the target of poaching in modern times. The reduction of rhino habitats has led to a sharp decline in the number of rhinos. At present, the Asian rhinoceros is almost extinct, and the number of existing African rhinoceros is not large. In order to save the rhinoceros facing extinction, the Indian rhinoceros and the white rhinoceros are listed as threatened species by the Red Book of Endangered Anima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 赵竹宋云李明福张永江鲁洁王仁睿许瑾
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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