Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof
A denitrifying bacteria and facultative anaerobic technology, applied in the field of facultative anaerobic denitrifying bacteria, can solve the problems of long time of granular sludge and limiting the application of AGS, and achieve stable effect, wide applicability and promotion of production Effect
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Embodiment 1
[0040] Embodiment 1 Isolation and identification of bacterial strains of the present invention:
[0041] (1) Medium:
[0042] A. Strain isolation, purification, preservation medium ( / L):
[0043] CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; Agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.
[0044] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L):
[0045] CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.
[0046] C. LB medium ( / L):
[0047] Peptone, 10g; yeast extract, 5g; sodium chloride, 10g; pH is controlled at 7.5.
[0048] (2) Isolation and purification of Pseudomonas stutzeri LZ-4 strain:
[0049] Weigh 10g of rhizosphere soil sample of Arundo donax and put it in a 250mL Erlenmeyer flask, add 100mL of sterile water and a few glass beads, and vibrate on a shaker for 15min to disperse the soil samp...
Embodiment 2
[0068] Embodiment 2 The cultivation of bacterial strain of the present invention
[0069] (1) Medium used
[0070] A. Strain preservation medium ( / L): peptone, 5g; yeast extract, 3g; glucose, 1g; NaCl, 6g; agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.
[0071] B. Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.
[0072] The above medium was sterilized at 121°C for 20 minutes before use.
[0073] (2) Culture conditions
[0074] Use an inoculation loop to scrape 1 ring of bacterial lawn from the Pseudomonas stutzeri LZ-4 preserved on the slant of the preservation medium, inoculate it into a 250mL Erlenmeyer flask containing 100mL of sterilized LB medium, and keep the temperature at 30°C Shake culture for 12 hours to obtain seed solution. During the experiment, the seed liquid was inoculated...
Embodiment 3
[0075] Example 3 The optimal denitrification conditions of the bacterial strain of the present invention
[0076] Put 100mL of sterilized denitrification medium into a 250mL Erlenmeyer flask, insert 10% of the inoculum into the seed bacterial solution, and let it stand for cultivation. In the range of 20-30°C, Pseudomonas stutzeri LZ-4 can remove 95% of total nitrogen, and the removal rate of total nitrogen is the highest at 25°C, reaching 97%. The bacterial strain of the invention can adapt to a relatively wide pH value, and can reduce the total nitrogen of about 150 mg / L to below 10 mg / L between pH 6-11 without nitrite accumulation. The optimum C / N (mol / mol) ratio of the strain of the invention is 3, and when C / N≥3, the removal rate of total nitrogen can reach over 90%. The slightly dissolved oxygen environment (DO≤0.5mg / L) of static culture is most suitable for the exertion of the denitrification activity of the strain of the present invention.
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