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Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment

A technology of Bacillus highlandi and high-salt wastewater, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problem of no bacterial species report, and achieve strong tolerance and cell productivity High, solve the effect of slow value-added

Active Publication Date: 2012-10-03
北京大学科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reports on high-salt wastewater at home and abroad focus on the research on the removal of organic matter, and there is no report on the strains that have the ability to remove nitrogen and phosphorus under high-salt conditions.

Method used

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  • Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment
  • Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment
  • Application of salt-tolerant nitrogen and phosphorus removing bacillus alitudinis to wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Implementation Example 1: Simultaneous Denitrification and Phosphorus Ability Determination of Bacillus altitudinis at a Salinity of 3%

[0045] Inoculate the strain of Bacillus altitudinis (preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC) with the preservation number CGMCC No.5948) in 1L of LB medium to prevent the invasion of miscellaneous bacteria and To maintain the growth vigor of the bacteria, carry out enrichment culture. The cultured bacterial solution was centrifuged, washed three times with sterile water, and the optical density (OD 600 ) for the bacterial suspension of 1-2.

[0046] Take 10mL of the above bacterial suspension and add two test media containing 90mL of 3% salinity (calculated as NaCl) (0.94g glucose, 0.153g NH 4 Cl, 0.035g KH 2 PO 4 , 0.1gMgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, pH 7.0~7.5) Erlenmeyer flasks, sealed with 9 layers of gauze, and cultured in a shaker at 30°C and 150rpm....

Embodiment 2

[0048] Implementation Example 2: Denitrification and phosphorus removal effects of strains under different salinity conditions

[0049] With glucose as carbon source and ammonia nitrogen as nitrogen source, the removal ability of Bacillus altitudinis strains described in the examples to ammonia nitrogen at different salinities was determined. The specific implementation steps are as follows:

[0050] Inoculate Bacillus altitudinis strains in 100ml of inorganic salt medium with salinity (calculated as NaCl) of 1%, 3%, 5%, 8%, 10% and 13%, respectively, at 30°C at 150rpm Pre-incubated in a shaker. When the strain grows to the late logarithmic growth phase, take 10ml of the bacterial solution and insert it into 90ml of fresh inorganic salts with a salinity (calculated as NaCl) of 1%, 3%, 5%, 8%, 10% and 13% for culture Culture medium was shaken at 30°C and 150 rpm in a shaker. The culture medium that was not inoculated with the bacterial suspension was used as a blank control ...

Embodiment 3

[0053] Implementation example 3: Denitrification and phosphorus removal experiments of bacterial strains under different pH conditions

[0054] The strain of Bacillus altitudinis was inoculated in 100 ml of inorganic salt medium with a salinity (calculated as NaCl) of 3%, and pre-cultured in a shaker at 30°C and 150 rpm. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into 90ml of fresh inorganic salt medium, adjust the pH of the medium to 6.5-7.0, 7.0-7.5 and 7.5-8.0 respectively, at 30°C Shaking culture was carried out in a shaker at 150 rpm. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. Take a small amount of reaction solution at 6h, 12h, 18h, 24h, 36h and 48h, a part of which is directly used to measure the optical density of the bacteria, and the rest is centrifuged at 8000rpm for 10min, and the supernatant is taken to measur...

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Abstract

The invention relates to a high salt-tolerant bacillus alitudinis with functions of heterotrophic nitrification-aerobic denitrification and phosphorous removal and application thereof to wastewater treatment. The bacillus alitudinis has strong high salt environment tolerance, grows well under a high-salt condition, and can metabolize by taking organic carbon as one and only carbon source and ammonia nitrogen as one and only nitrogen source; through the heterotrophic nitrification-aerobic denitrification, the ammonia nitrogen is directly converted into a gaseous product so as to achieve a denitrifying purpose; the bacillus alitudinis can also take nitrate nitrogen as one and only nitrogen source, the nitrate nitrogen is converted into a gaseous product through the aerobic denitrification; and under aerobic conditions inorganic phosphorus can be taken into a body and converted into a component of the body so as to realize a purpose of removing phosphorus in sewage. The bacillus alitudinis is applied to high-salt wastewater treatment, can achieve synchronization removal of nitrogen and phosphorus under a single aerobic condition, and provides a new way for solving a problem of biological removal of the nitrogen and phosphorus under the high-salt condition.

Description

technical field [0001] The invention relates to a strain of Bacillus altitudinis and its application in high-salt wastewater treatment. The strain has the function of heterotrophic nitrification-aerobic denitrification, and can realize the process of simultaneous nitrification and denitrification denitrification under high-salt conditions, and at the same time complete the removal of phosphorus-containing pollutants in sewage. Background technique [0002] At present, the eutrophication pollution of various water bodies in our country is becoming more and more serious, and the problem of blue-green algae red tides occurs frequently, which has caused huge damage to water body safety and human health. The excess of nutrients such as nitrogen and phosphorus is the root cause of water eutrophication. When the concentration of N in the water body is >0.2mg / l, and the concentration of P is >0.02mg / l, there is a possibility of eutrophication. Domestic sewage is the largest d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/16C02F101/10
Inventor 倪晋仁邓若男
Owner 北京大学科技开发有限公司
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