Micro/nano-fiber bone repairing scaffold and production method thereof
A technology for micro-nano fiber and bone repair, which is applied in fiber processing, spinning solution preparation, medical science, etc., can solve the problems such as the decline of repair effect, and achieve the effect of being conducive to repair, high activity, and optimized release conditions
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Embodiment 1
[0062] Preparation of the internal oil phase: Weigh 10 μg of growth factor BMP-2 and fully dissolve it in 1 g of aqueous solution; weigh 0.5 g of polylactic acid and dissolve it in 10 g of oil phase with a volume ratio of dichloromethane to acetone of 2 / 1. Mix the two solutions of water phase and oil phase to form a mixture, and fully emulsify with a high-speed homogenizer for 1 min. Add the emulsion to 50 g aqueous solution of polyethylene glycol containing 0.3 g, slowly add and ultrasonically disperse, and then stir at a low speed for 3 hours in an environment of 25° C. to volatilize the organic solvent to obtain polylactic acid particles containing growth factors.
[0063] Put the polylactic acid particles into a 25% isopropanol mixed aqueous solution containing 350 mM sodium chloride, 15 mM calcium chloride, 10 mM disodium hydrogen phosphate, 20 mM sodium bicarbonate, and 15 mM magnesium chloride. After standing for 24 hours, a layer of calcium phosphate shell was formed o...
Embodiment 2
[0067] a, the preparation sodium dodecyl sulfonate content is 20mM, the solution that chitosan or gelatin content is 0.15g / L, adjusts pH value to 10.5, obtains solution A;
[0068] b. Prepare a calcium salt solution with a calcium ion concentration of 500 mM, adjust the pH value to 10.5, and obtain solution B;
[0069] c. Prepare a sodium dihydrogen phosphate solution with a sodium dihydrogen phosphate content of 500 mM, add 0.3 times the relative volume of isopropanol, adjust the pH value to 10.5, and obtain solution C;
[0070] d. According to the volume ratio A:B:C=2:1:1, add solution B into solution A, after stirring evenly, slowly add solution C dropwise, the addition speed of solution C is 1ml / min, the dropwise addition process The middle stirring speed is controlled to 500rpm until no more precipitation occurs, and the precipitation is the polymer particles with an intercalation structure.
[0071] Put the polymer particles with intercalation structure into sod...
Embodiment 3
[0075] a, preparing sodium dodecyl sulfonate content is 20mM, chitosan or gelatin content is 0.15g / L solution, and add the growth factor of BMP-7, adjust pH value to 10.5, obtain solution A;
[0076] b. Prepare a calcium salt solution with a calcium ion concentration of 500 mM, adjust the pH value to 10.5, and obtain solution B;
[0077] c. Prepare a sodium dihydrogen phosphate solution with a sodium dihydrogen phosphate content of 500 mM, add 0.3 times the relative volume of isopropanol, adjust the pH value to 10.5, and obtain solution C;
[0078] d. According to the volume ratio A:B:C=2:1:1, add solution B into solution A, after stirring evenly, slowly add solution C dropwise, the addition speed of solution C is 1ml / min, the dropwise addition process The middle stirring speed is controlled to 500rpm until no more precipitation occurs, and the precipitation is the polymer particles with an intercalation structure.
[0079] Put the polymer particles with intercalation ...
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