Extraction method of glucan in fig leaf
A fig leaf and extraction method technology, applied in the field of glucan extraction, can solve the problems of fig leaf waste and failure to be developed and utilized, and achieve the effects of mild extraction conditions, effective value-added utilization, and low production cost
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Embodiment 1
[0019] Example 1: Harvest the fresh fig leaves with stalks in August, wash them with flowing water, send them to a tissue grinder for grinding, and then grind the ground fig leaves with a colloid mill, add 2 times of water and grind them into 100 The target slurry is then transferred to an ultrasonic reaction kettle, add 7 times of water, heat to 55°C for ultrasonic treatment, ultrasonic power is 800W, ultrasonic treatment is performed for 3 hours, and then the supernatant is collected by centrifugation and concentrated under reduced pressure to obtain a concentrated solution. Then add ethanol until the volume concentration of ethanol is 80% for alcohol precipitation, take the precipitate and add 9 times the mass of water to redissolve, perform ultrafiltration with a membrane with a molecular cut-off of 10,000, and concentrate the retentate under reduced pressure to 1 / 3 of its original volume Afterwards, freeze-dry to a moisture content of 3% to obtain the β-glucan of the pres...
Embodiment 2
[0020] Example 2: Harvest the fresh fig leaves with stalks in July, wash them with flowing water, send them to a tissue grinder for grinding, then grind the ground fig leaves with a colloid mill, add 2 times of water and grind them into 100 The target slurry is then transferred to an ultrasonic reactor, add 6 times of water, heat to 52°C for ultrasonic treatment at a constant temperature, the ultrasonic power is 500W, and the ultrasonic is applied for 4 hours, then the supernatant is collected by centrifugation and concentrated under reduced pressure to obtain a concentrated solution. Then add ethanol until the volume concentration of ethanol is 80% to carry out alcohol precipitation. After alcohol precipitation, the slurry is vacuum-dried to a moisture content of 4% at a temperature of 70°C and a pressure of 0.08Mpa to obtain the dextran of the present invention.
Embodiment 3
[0021] Example 3: Harvest the fresh fig leaves with stalks in September, wash them with running water and send them to a tissue grinder for grinding, then grind the ground fig leaves with a colloid mill, add water 3 times and grind them into 100 The target slurry is then transferred to an ultrasonic reaction kettle, added 5 times of water, heated to a constant temperature of 52°C for ultrasonic treatment, and the ultrasonic power is 800W, and the ultrasonic is applied for 3 hours, and then the supernatant is collected by centrifugation and concentrated under reduced pressure to obtain a concentrated solution. Then add ethanol until the volume concentration of ethanol is 80% for alcohol precipitation, take the precipitate and add 7 times the mass of water to redissolve, perform ultrafiltration with a membrane with a molecular cut-off of 10,000, and concentrate the retentate under reduced pressure to 1 / 3 of its original volume Afterwards, freeze-dry to a moisture content of 3% ...
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