Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rhoifolin extraction method and usage of drug prepared by rhoifolin

A technology of Rhodorin and its extraction method, which is applied in the field of Rhodorin extraction, and can solve the problems of high price and the like

Inactive Publication Date: 2012-08-15
JIANGXI SHANXIANG PHARMA +1
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Western medicine "Oseltamivir Phosphate (Tamiflu, (3R, 4R, 5S)-4-Acetamide-5-Amino-3(1-Propoxyethyl)-1-Cyclohexene-1 Carboxylic Acid Ethyl Phosphate "Salt)" is a neuraminidase inhibitor, which has a specific inhibitory effect on the entry of influenza virus into cells, but it is not only expensive, but also some patients experience vomiting, nausea, insomnia, headache, abdominal pain, diarrhea, dizziness, fatigue, Side effects such as nasal congestion, sore throat, and coughing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhoifolin extraction method and usage of drug prepared by rhoifolin
  • Rhoifolin extraction method and usage of drug prepared by rhoifolin
  • Rhoifolin extraction method and usage of drug prepared by rhoifolin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 1000g of the round leaves of Shanxiang, add 12 times the amount of 70% ethanol for reflux extraction for 2 hours, filter, add 10 times the amount of 70% ethanol for reflux extraction for 1.5 hours, filter, combine the two extracts, recover the ethanol, and pass the aqueous solution through the The treated D101 macroporous resin column was eluted with water, 10% ethanol, 45% ethanol, and 1% sodium hydroxide solution respectively, and the eluted part of 45% ethanol was collected, concentrated under reduced pressure, and dried to obtain more than 30% rhodium The mixed total glycosides were separated by silica gel column chromatography and Sephadex LH-20 column chromatography, and the Rhodorin fractions were combined and crystallized to obtain the pure Rhodoglucoside (1.5g, purity: 98.5%). Standard UV, IR, ESI-MS, 1 H-NMR, 13 Compared with C-NMR, the structure of Rhodorin was determined.

Embodiment 2

[0032]Take 1000g of the round leaves of Shanxiang, add 10 times the amount of 60% ethanol for reflux extraction for 2 hours, filter, add 8 times the amount of 60% ethanol for reflux extraction for 1.5 hours, filter, combine the two extracts, recover the ethanol, and pass the aqueous solution through the The treated D101 macroporous resin column was eluted with water, 10% ethanol, 45% ethanol, and 0.8% sodium hydroxide solution respectively, and the eluted part of 45% ethanol was collected, concentrated under reduced pressure, and dried to obtain more than 30% rhodium The mixed total glycosides were separated by silica gel column chromatography and Sephadex LH-20 column chromatography, and the rhodium glucoside fractions were combined and crystallized to obtain pure rhodium rhoside (1.6g, purity: 98.7%). Standard UV, IR, ESI-MS, 1 H-NMR, 13 Compared with C-NMR, the structure of Rhodorin was confirmed.

Embodiment 3

[0034] Take 1000g of the round leaves of Shanxiang, add 15 times the amount of 60% ethanol for reflux extraction for 2 hours, filter, add 12 times the amount of 60% ethanol for reflux extraction for 1.5 hours, filter, combine the two extracts, recover the ethanol, and pass the aqueous solution through the The treated D101 macroporous resin column was eluted with water, 8% ethanol, 40% ethanol, and 1.2% sodium hydroxide solution respectively, and the eluted part of 40% ethanol was collected, concentrated under reduced pressure, and dried to obtain more than 30% rhodium The mixed total glycosides were separated by silica gel column chromatography and Sephadex LH-20 column chromatography, and the rhodium glucoside fractions were combined and crystallized to obtain the pure rhodium rhoside (1.7g, purity: 98.9%). Standard UV, IR, ESI-MS, 1 H-NMR, 13 Compared with C-NMR, the structure of Rhodorin was confirmed.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to rhoifolin and usage of a drug prepared by the rhoifolin. The rhoifolin extraction method includes the steps: taking folium turpiniae or pummelo peel, adding 5-15 times of amount of ethanol with the concentration of 30%-90%, performing reflux extraction for 1-3 times, each reflux extraction takes 1-3 hours and filtering; and combining filtrate, recovering the ethanol, eluting water solution by macroporous resin, collecting eluted portions, depressurizing, concentrating and drying to obtain mixed total glycoside of the rhoifolin prior to separation, combining rhoifolin fluid parts, crystallizing and obtaining a rhoifolin pure product. The rhoifolin can serve as neuraminidase inhibitor to prevent and treat influenza, and can be made into dosage forms acceptable in pharmacy.

Description

technical field [0001] The invention relates to a method for extracting rhodol and its use in the preparation of medicines. Background technique [0002] At present, influenza virus is becoming more and more serious, and influenza virus is closely related to respiratory diseases and systemic diseases caused by it. my country is one of the countries with high incidence of influenza. Not only has a large population, but also living habits are conducive to the transmission of influenza virus. The average number of episodes ranged from 0.3 to 0.7, and 2 to 4 in key groups. Influenza poses a serious threat to human beings, especially the emerging strains of influenza virus, which not only cause secondary infections of other microorganisms, but also directly lead to organ damage and allergic reactions leading to death. But there is no effective method and treatment means for treating influenza virus and its disease (influenza) at present, such as treating the inflammatory response...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P31/16A61P13/12A61P11/00
Inventor 杨小玲王燕平谢宁叶劲英李志勇吕武清刘地发程帆蔡永红
Owner JIANGXI SHANXIANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com