Protein chip used for detecting human ferrum reserve and reagent kit thereof
A technology of protein chip and detection of human body, which is applied in the field of protein chip, can solve the problems such as undiscovered protein chip, and achieve the effect of stable detection result, high sensitivity and simple detection method
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Embodiment 1
[0035] Example 1 Preparation of a protein chip for detecting iron reserves in human body
[0036] Proceed as follows:
[0037] (1) Selection of solid phase carrier: choose a three-dimensional substrate in the form of an aldylated 12-lattice matrix (the crystal core polymer three-dimensional substrate, purchased from Boao Biological Co., Ltd.), and determine the arrangement of the lattice to be 3×6 array.
[0038] (2) Prepare sample solution:
[0039] a. Use 50% glycerol spotting buffer containing free Cy3 (purchased from Boao Biological Co., Ltd.) and PBS buffer of pH 7.4 according to the volume of spotting buffer: PBS: monoclonal antibody 1:1:2 Prepare respectively: mouse anti-serum ferritin monoclonal antibody (Anti-Ferritin, mitochondrial antibody [F23], abcam company) sample solution, mouse anti-soluble transferrin receptor monoclonal antibody (Anti-Transferrin Receptor antibody [ 2B6], abcam Company) sample solution and mouse anti-C reactive protein monoclonal antibody...
Embodiment 2
[0047] Example 2 A protein chip used to detect iron reserves in the human body Contrast test for detecting iron reserves in the human body
[0048] The test samples were the serum collected from voluntary subjects (20-60 years old) of adult women of childbearing age in Nanyang, Henan Province in 2010. Six cases (numbered I, II, III, IV, V, VI) were randomly selected. The sample is divided into two parts, one part is 200 μl / part, and the contents of serum ferritin, soluble transferrin receptor and C-reactive protein are detected by turbidimetry; the other part is 20 μl / part, which is used in the embodiment of the present invention 1 The prepared protein chip was used to detect serum ferritin, soluble transferrin receptor and C-reactive protein at the same time.
[0049] (1) Nephelometric test: 200 μl of serum was taken from each case and tested by turbidimetric test. Nephelometric detection was carried out according to conventional methods (Dubois S et al. GIT Labor-Medizin, 1...
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