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Human serum albumin nano biomaterial and preparation method thereof

A technology of human serum albumin and nanofibers, applied in the direction of spinning solution preparation, single-component protein rayon, application, etc., to achieve the effect of simple production

Active Publication Date: 2012-08-01
HANGZHOU YAHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although there have been many recent reports using human albumin and glutaraldehyde as cross-linking agents to produce nanoparticles as drug sustained-release agents (Abbasi S, Paul A, Shao W, Prakash S.2012.Cationic albumin nanoparticles for enhanced drug delivery to treat breast cancer: preparation and in vitro assessment. JDrug Deliv. 2012: 686108, Epub 2011 Dec 8; Sebak S, Mirzaei M, Malhotra M, Kulamarva A, Prakash S. 2010. Human serum albumin nanoparticles as an efficient noscapine drug delivery system for potential use Breast cancer: preparation and in vitroanalysis. Int J Nanomedicine.5: 525-32.; Langer K, Balthasar S, Vogel V, Dinauer N, von Briesen H, Schubert D. 2003. Optimization of the preparation process for human serum malbumin (HSA ) nanoparticles.Int J Phar.257:169-80), but human albumin as a matrix material to produce materials that can meet medical mechanical and physical strength requirements has not been reported

Method used

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  • Human serum albumin nano biomaterial and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Prepare a solution system for dissolving albumin: add 0.27ml of 0.1M ammonium hydroxide to 1.74ml of trifluoroethanol, then add 50ul of mercaptoethanol, stir to make it fully mixed. Weigh 0.3 g of recombinant human serum albumin (10%, w / v) and dissolve it in the above solution, stir gently with a magnetic bar for 30 minutes until the albumin is fully dissolved, and pump air with a vacuum pump for 30 minutes to eliminate the bubbles to obtain spinning solution.

[0046] The spinning solution is formed on a collector with a flow rate of 0.3ml per hour through the spinneret hole, an electric field intensity of 1.0KV / cm, a distance between the electrode and the rotating disk of 14cm, and a collector rotating disk edge speed of 8 m / s. After curing and drying for 24 hours in a glass drying dish (humidity 30%), human albumin nanofibers were obtained.

[0047] The albumin fibers were then soaked in deionized hot water at 37°C for 1 hour, then soaked in 35% ethanol prepared fro...

Embodiment 2

[0050]Albumin derived from human plasma (non-genetic recombination) was selected as the matrix material. Prepare a solution system for dissolving albumin: add 0.27ml of 0.1M ammonium hydroxide to 1.74ml of trifluoroethanol, then add 50ul of mercaptoethanol, stir to make it fully mixed. Weigh 0.3 g of recombinant human serum albumin (10%, w / v) and dissolve it in the above solution, stir gently with a magnetic bar for 30 minutes until the albumin is fully dissolved, and pump air with a vacuum pump for 30 minutes to eliminate the bubbles to obtain spinning solution.

[0051] The spinning solution is formed on a collector with a flow rate of 0.3ml per hour through the spinneret hole, an electric field intensity of 1.0KV / cm, a distance between the electrode and the rotating disk of 14cm, and an edge speed of the collector rotating disk of 8 m / s. After curing and drying for 24 hours in a glass drying dish (humidity 30%), human albumin nanofibers were obtained.

[0052] The albumin...

Embodiment 3

[0055] Prepare a solution system for dissolving albumin: add 0.16ml of 0.1M ammonium hydroxide to 1.85ml of trifluoroethanol, then add 50ul of mercaptoethanol, stir to make it fully mixed. Weigh 0.37 g of recombinant human serum albumin (12%, w / v) and dissolve it in the above solution, stir gently with a magnetic bar for 30 minutes until the albumin is fully dissolved, and pump air with a vacuum pump for 30 minutes to eliminate the bubbles to obtain spinning solution.

[0056] The spinning solution is formed on a collector with a flow rate of 0.3ml per hour through the spinneret hole, an electric field intensity of 1.0KV / cm, a distance between the electrode and the rotating disk of 14cm, and an edge speed of the collector rotating disk of 8 m / s. After curing and drying for 24 hours in a glass drying dish (humidity 30%), human albumin nanofibers were obtained.

[0057] The albumin fibers were then soaked in deionized hot water at 37°C for 1 hour, then soaked in 35% ethanol pre...

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Abstract

The invention discloses a spinning solution and a method for preparing human serum albumin nanofibers or films by using the spinning solution. The method comprises the following steps of: mixing human serum albumin, trifluoroethanol, ammonium hydroxide or hydrochloric acid, and beta-mercatoethanol in a ratio to obtain the spinning solution; forming by using an electric spinner or a film pressing machine; and curing and drying to obtain the nanofibers, films and other solid objects respectively. After the human serum albumin materials are soaked in hot water and ethanol, and dried, the human serum albumin materials have high-strength physical and mechanical properties and are suitable for replacing the conventional clinical products, such as medical degradable suture lines, orthopaedic materials, intracavity and in-vitro hemostatic gauze, antiadhesive membranes and other medical auxiliary materials.

Description

technical field [0001] The invention relates to the field of medical biological materials, in particular to a human serum albumin nano-biological material that does not cause immune reactions to human bodies and a preparation method thereof. Background technique [0002] The field of medical absorbable materials is a hot spot in the world's medical field, and the market is huge. At present, only European and American countries have an annual output value in this field that has exceeded 5 billion US dollars, and the growth is rapid. Medical materials not only need to have medical effects, but also have good biocompatibility and considerable physical and mechanical properties. The degradation products can be absorbed and utilized by the body, or can be excreted through normal metabolism, and have the degradation required for practical applications. rate. Biodegradable materials have been widely used in surgical sutures, artificial skin, orthopedics, in vivo drug release agent...

Claims

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Application Information

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IPC IPC(8): D01F4/00D01D1/02D01D5/00C08L89/00A61L15/32A61L15/64A61L17/10A61L24/10A61L27/58A61L27/60A61L31/04A61L31/14
Inventor 徐建华
Owner HANGZHOU YAHUI BIOTECH CO LTD
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