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Biological method for producing resveratrol

A technology of resveratrol and its production method, which is applied in the field of biomedicine, can solve the problems of complex steps, low production capacity, and low yield, and achieve the effects of facilitating industrial production, solving source problems, and reducing production costs

Inactive Publication Date: 2014-07-30
TIANJIN UNIV
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Problems solved by technology

However, it still cannot solve the fundamental problems faced by the plant extraction method, such as limited plant raw materials and unstable quality, low production capacity, planting is limited by seasons and geographical locations, etc.
Although the chemical total synthesis of resveratrol has been reported, the steps are complicated, the yield is low, and the environmental pollution is serious

Method used

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  • Biological method for producing resveratrol
  • Biological method for producing resveratrol
  • Biological method for producing resveratrol

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Embodiment Construction

[0023] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. Using JCAT online gene design software, based on published gene sequences, conservatively design and optimize phenylalanine hydroxylase gene (PAH), tyrosine ammonia lyase gene (TAL), 4-coumaroyl-CoA ligase gene (4CL) and resveratrol synthase (RS) gene sequence, and these target genes were fully synthesized, wherein FASTpfu enzyme was purchased from Beijing Quanshijin Biotechnology Co., Ltd., and restriction enzymes were purchased from Fushitai Si Biotechnology (Shenzhen) Co., Ltd.

[0024] Sequence Optimization and Acquisition of Phenylalanine Hydroxylase Gene (PAH)

[0025] Preferably the phenylalanine hydroxylase gene sequence of Pseudomonas stutzeri was codon-optimized using jcat (http: / / www.jcat.de) for use in Escherichia coli BW25113 and Corynebacterium glutamicum For the expression of ATCC21673, the length of the optimized gene is 786bp, the...

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Abstract

The invention discloses a biological method for producing resveratrol, which comprises the following steps: ligating target genes obtained by restriction enzyme digestion to an expression vector, wherein the target genes include the sequences of phenylalanine hydroxylase (PAH), tyrosine ammonia lyase (TAL) 4-coumarate:coenzyme A ligase (4CL) and resveratrol synthase (RS); transforming the constructed expression vector into a strain to obtain a recombinant engineered strain; and fermenting the recombined engineered strain. Compared with the prior art, the technical scheme provided by the invention uses the genetically engineered strain for fermentation to produce resveratrol, so as to realize denovo synthesis of resveratrol with no need of adding substrates. The method disclosed by the invention solves the source problem of resveratrol, and at the same time, reduces the production cost in a maximum extent, and is beneficial for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically, relates to a method for producing resveratrol. Background technique [0002] Resveratrol (resveratrol, res), its chemical name is 3,4', 5-trihydroxy-trans-stilbene, also known as stilbene triphenol, and its molecular formula is C 14 h 12 o 3 , the molecular weight is 228.2. The pure product is a colorless needle-like crystal, insoluble in water (0.03g / L at 25°C), easily soluble in organic solvents such as ethanol, acetone, and ethyl acetate. With the deepening of research on resveratrol, it was found that resveratrol has a wide range of physiological and pharmacological activities, including anticancer activity, antioxidation, cardiovascular protection, free radical scavenging, anti-inflammatory, antibacterial, Immunomodulatory effects, estrogen-like activity, etc. Therefore, resveratrol has broad market prospects in the fields of health care products, cosmetics, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/22C12N15/53C12N15/55C12N15/52C12N15/63C12N1/21C12R1/19C12R1/15
Inventor 赵广荣王长松姚元锋
Owner TIANJIN UNIV
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