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Fusarium avenaceum as well as agent and application of fusarium avenaceum

A technology for Fusarium and oats, which is applied in the field of agricultural microorganisms, can solve problems such as poor control effect, and achieve the effects of low cost, low residual environment and simple preparation method.

Inactive Publication Date: 2012-07-25
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large amount of wild oat seed production, the seeds can germinate continuously in the soil for 4 to 5 years, and some can still germinate after burning and livestock stomachs and intestines, which makes the control effect of the prior art measures very poor.

Method used

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  • Fusarium avenaceum as well as agent and application of fusarium avenaceum
  • Fusarium avenaceum as well as agent and application of fusarium avenaceum
  • Fusarium avenaceum as well as agent and application of fusarium avenaceum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of the liquid preparation of GD-2 microbial bacterial agent

[0033] (1) Strain activation: activate the low-temperature-preserved strain GD-2 on PDA plate medium (25°C), and multiply it for later use;

[0034] (2) Preparation of seed liquid: Prepare PDB liquid medium according to the conventional method, fill 150mL PDB culture medium (PDB medium: potato 200g, glucose 20.0g, distilled water 1000mL) into a 500mL Erlenmeyer flask, autoclave with moist heat, wait for temperature After cooling down to room temperature, insert the activated GD-2 strain in step (1) into each bottle, 3 pieces of bacterial slices with a diameter of 0.5 cm, and carry out shaking culture on a shaker at 24-28 ° C and a speed of 150 rpm for 48-72 hours to obtain seed liquid;

[0035] (3) Liquid fermentation medium: Glucose and urea are used as carbon and nitrogen sources respectively (C / N is 6:1~5:1), other components and dosages are the same as Czapek culture medium,...

Embodiment 2

[0038] Embodiment 2: the preparation of the wettable powder of GD-2 microbial bacterial agent

[0039]Steps (1) to (4) are the same as in Example 1, and then the obtained fermentation liquid of the GD-2 strain is filtered to remove mycelium, and then the filtrate is extracted with petroleum ether, and the extract is concentrated under reduced pressure with a rotary evaporator to obtain an extract. Weigh 1.5 grams of extract, add dispersant sodium carboxymethyl cellulose (0.111 grams), sodium dodecylbenzenesulfonate (0.115 grams), diatomaceous earth (2.008 grams) and mix well, continue grinding to 325 mesh sieve That is, the wettable powder of GD-2 bacterial agent is obtained.

Embodiment 3

[0040] Embodiment 3: Greenhouse control effect test of Fusarium oats GD-2

[0041] The experiment was carried out in the greenhouse of the Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences. First, the wild oats were germinated. When the wild oat seeds were white, 30 seeds were planted in each pot. When the seedlings grew to 2 to 3 leaves. According to the difference of the dilution concentration of the GD-2 liquid preparation prepared in the embodiment 1 applied, it is divided into 3 concentration treatments:

[0042] Process 1: about 1.0 x 10 6 conidia / mL

[0043] Process 2: Approx. 1.0×10 7 conidia / mL

[0044] Process 3: about 1.0 x 10 8 conidia / mL

[0045] CK: GD-2 liquid preparation was not added as a control

[0046] Each pot was sprayed with 60mL of GD-2 liquid preparations of the above different treatments, and each treatment was repeated 4 times. The number of wild oat plants and fresh weight were investigated 20 days and 40 days...

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Abstract

The invention belongs to the field of agricultural microorganisms and discloses a Fusarium avenaceum strain GD-2, and the preservation number of the strain is CGMCCNo.5579. The invention also discloses a microorganism agent prepared by using the GD-2. The Fusarium avenaceum GD-2 and the agent of the fusarium avenaceum GD-2 have good inhibiting effect on wild oats in a rape field, and the prevention effect in the field reaches 74.37 %. Secondly, the preparation method of the microorganism agent of the fusarium avenaceum is simple, low in cost and is easy for popularization and application. In addition, the fusarium avenaceum belongs to a biological prevention method which has no pollution and low residue and is environment-friendly.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a Fusarium avenatum, and a microbial agent produced by using the Fusarium avenatum and its application. Background technique [0002] wild oats ( Avena fatua L.) belongs to Poaceae (Poaceae), Avena (Avena), is an annual herb, also known as bell wheat. It is distributed in all provinces of China. There are 34 species in this genus. The plant height is 30-150 cm. Fibrous roots; stems clustered; leaf sheaths loose, ligules large and transparent; panicles; caryopsis fusiform. Wild oats are weeds associated with wheat. Due to the same environmental conditions and similar seedling shapes, wild oats are difficult to control and extremely harmful. The growth habit of wild oats is not only similar to that of wheat, but also has inconsistent emergence, vigorous growth and high reproductive rate. Ripens earlier than wheat. After wheat was harmed by wild oats, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P13/02C12R1/77
Inventor 程亮郭青云朱海霞魏有海翁华郭良芝
Owner QINGHAI UNIVERSITY
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