Preparation method of biotin-modified and rare earth-doped inorganic fluorescent nanoparticles

Abstract

The invention discloses a preparation method of biotin-modified and rare earth-doped inorganic fluorescent nanoparticles. The method comprises the following steps: activating the carboxyl groups of biotin in a solvent of anhydrous N,N-dimethylformamide with benzotriazole-N,N,N',N'-tetramethylurea hexafluorophosphate (HBTU) and N,N-diisopropylethylamine (DIEA) as activators, reacting the activated carboxyl group with the amino groups of the nanoparticles to form amide bonds so as to achieve biotin modification on the surface of rare earth-doped inorganic fluorescent nanoparticles. The biotin-modified and rare earth-doped inorganic fluorescent nanoparticles prepared with the method provided by the invention can quickly and stably bind with avidins, and the specific luminescence of rare earth ions doped in the nanoparticles can give sensitive response to the binding, which indicates that the nano materials obtained by the preparation method have potentials of applications in the fields of biological labeling and immunological analysis.

Description

Technical field [0001] The invention relates to a preparation method of biotin-modified inorganic nano materials, in particular to a preparation method of biotin-modified rare earth-doped inorganic fluorescent nano particles. Background technique [0002] In recent years, rare earth-doped inorganic fluorescent nanoparticles have attracted people's attention due to their high physical and chemical stability and excellent luminescence performance. They are widely used in information display, green lighting, biomarkers, solar cells and other fields. Especially when applied to biomarkers, compared with commonly used fluorescent dyes and semiconductor quantum dots, inorganic rare earth nano-luminescent materials have comprehensive advantages such as high luminous efficiency, low toxicity, narrow line width, long fluorescence lifetime and tunable emission wavelength. , Is currently a new generation of fluorescent biomarker materials that are generally promising. In the field of biomar...

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Problems solved by technology

The basis for research in this field is how to connect biotin or avidin to the surface of nanoparticles. The existing research results usually use 1-ethyl-3-(3-dimethylaminopropyl)-carbonization Diimine (EDC) and N-hydroxysuccinimide (NHS) are used as cross-linking agents to connect biotin and inorganic nanoparticles with functional groups such as amino or carboxyl groups on the surface in the "water phase" environment of the buffer solution. In this linking process, the solubility of biotin, avidin and nanoparticles in the buffer solution is an important factor affecting the reaction yield; at the same time, EDC also has the problem of hydrolysis of intermediate products, which limits the scope of this linking reaction. Yield

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