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Preparation method of biotin-modified rare earth-doped inorganic fluorescent nanoparticles

A nanoparticle and rare earth doping technology, applied in biological testing, chemical instruments and methods, luminescent materials, etc., can solve problems such as limiting yield, and achieve the effects of avoiding hydrolysis, simple preparation process, and good repeatability

Active Publication Date: 2016-07-06
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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Problems solved by technology

The basis for research in this field is how to connect biotin or avidin to the surface of nanoparticles. The existing research results usually use 1-ethyl-3-(3-dimethylaminopropyl)-carbonization Diimine (EDC) and N-hydroxysuccinimide (NHS) are used as cross-linking agents to connect biotin and inorganic nanoparticles with functional groups such as amino or carboxyl groups on the surface in the "water phase" environment of the buffer solution. In this linking process, the solubility of biotin, avidin and nanoparticles in the buffer solution is an important factor affecting the reaction yield; at the same time, EDC also has the problem of hydrolysis of intermediate products, which limits the scope of this linking reaction. Yield

Method used

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  • Preparation method of biotin-modified rare earth-doped inorganic fluorescent nanoparticles
  • Preparation method of biotin-modified rare earth-doped inorganic fluorescent nanoparticles
  • Preparation method of biotin-modified rare earth-doped inorganic fluorescent nanoparticles

Examples

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example 1

[0025] Example 1: NaYF 4 : Preparation of 5% Ce, 5% Tb nanoparticles surface-linked biotin. Weigh 30 mg of benzotriazole-N, N, N', N'-tetramethyluronium hexafluorophosphate (HBTU) and 30 mg of biotin dissolved in 0.9 mL of N, N-dimethylformamide (DMF ), adding 0.1 mL of N, N-diisopropylethylamine (DIEA) to activate the carboxyl group of biotin for 12 hours, and then adding 30 mg of NaYF with amino groups on the surface 4: 5% Ce, 5% Tb nanoparticles, after 12 hours of shaking reaction, wash the nanoparticles produced by the reaction with DMF and deionized water several times, and dry them in a vacuum oven to obtain NaYF surface-modified biotin 4 : 5% Ce, 5% Tb inorganic nanoparticles.

example 2

[0026] Example 2: NaYF 4 : Preparation of 20% Yb, 2% Er nanoparticles surface-linked biotin. Weigh 75mg of HBTU and 50mg of biotin dissolved in 1.8mL of DMF, add 0.2mL of DIEA to activate the carboxyl group of biotin for 8 hours, then add 50mg of NaYF with amino groups on the surface 4 : 20% Yb, 2% Er nanoparticles, after 8 hours of shaking reaction, wash the nanoparticles produced by the reaction with DMF and deionized water several times, and dry them in a vacuum oven to obtain NaYF surface-modified biotin 4 : 20% Yb, 2% Er inorganic nanoparticles.

example 3

[0027] Example 3: NaYF 4 : Preparation of 1% Dy nanoparticle surface-linked biotin. Weigh 10mg of HBTU and 20mg of biotin dissolved in 0.9mL of DMF, add 0.1mL of DIEA to activate the carboxyl group of biotin for 24 hours, then add 15mg of NaYF with amino groups on the surface 4 : 1% Dy nanoparticles, after shaking for 24 hours, the nanoparticles produced by the reaction were washed with DMF and deionized water several times, and dried in a vacuum oven to obtain NaYF surface-modified biotin 4 : 1% Dy inorganic nanoparticles.

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Abstract

The invention discloses a preparation method of biotin-modified rare earth-doped inorganic fluorescent nanoparticles. Using benzotriazole-N, N, N', N'-tetramethyluronium hexafluorophosphate (HBTU) and N, N-diisopropylethylamine (DIEA) as activators, in anhydrous N , in the solvent of N-dimethylformamide, the carboxyl group of biotin is activated, and then reacts with the amino group of nanoparticles to form an amide bond, so as to realize the biotin modification on the surface of rare earth-doped inorganic fluorescent nanoparticles. The biotin-modified rare-earth-doped inorganic fluorescent nanoparticles prepared by this method can be quickly and stably connected to avidin, and at the same time, due to the specific luminescence of the rare-earth ions doped in the nanoparticles, it can respond sensitively to this connection, indicating that The potential of nanomaterials obtained by this preparation method in the fields of biomarkers and immunoassays is demonstrated.

Description

technical field [0001] The invention relates to a preparation method of biotin-modified inorganic nanometer materials, in particular to a preparation method of biotin-modified rare earth-doped inorganic fluorescent nanoparticles. Background technique [0002] In recent years, rare earth-doped inorganic fluorescent nanoparticles have attracted attention because of their high physical and chemical stability and excellent luminescent properties, and have been widely used in information display, green lighting, biomarkers, solar cells and other fields. Especially when applied to biomarkers, compared with the currently commonly used fluorescent dyes and semiconductor quantum dots, inorganic rare earth nanoluminescent materials have comprehensive advantages such as high luminous efficiency, low toxicity, narrow line width, long fluorescence lifetime and tunable emission wavelength. , is a new generation of fluorescent biomarker materials that are generally favored at present. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/85G01N33/53
Inventor 陈学元涂大涛周山勇刘永升李仁富朱浩淼
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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