Detergent, cleaning agent or disinfectant containing hydrolyzates made of plant extracts
A technology of hydrolyzate and extract, applied in the directions of plant growth regulators, animal repellents, detergent compounding agents, etc., can solve problems such as no longer effective
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[0076] Preparation of test solution
[0077] Single drug and mixed extract with variable drug composition at 50% EtOH / H 2 In O (v / v, about 1 g of plant material for 20 ml of solvent), extract at room temperature for 24 hours while stirring. 1.6 ml of the extract was mixed with 320 μl 25% HCl (equivalent to 8.1 mol / L) and 80 μl 50% EtOH, and hydrolyzed at 90° C. for 45 minutes. For the purpose of comparison, in the second step, 1 ml of the extract was hydrolyzed under the same conditions while adding 1 ml of 25% HCl. After the extract was concentrated by evaporation, the residue was introduced into 1 ml of sterile water and tested for its antibacterial effect. Screening method: 80 μl of the test solution is placed on a Müller Hinton agar plate or a Müller Hinton agar plate with 5% sheep blood (which contains an unknown concentration of the bacteria to be tested) and incubated at 37° C. for 24 hours.
[0078] Spiral Platter: The bacterial flora is suspended in 5ml CASO-Bouillon and...
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