Method for decelerating nucleic acid molecules in solid nanopore

Abstract

The invention discloses a method for reducing perforation speed of nucleic acid molecules in a nanopore sequencing method. The method comprises the following steps of: adding the nucleic acid molecules to be measured into a nanopore sequencing device filled with electrolyte, wherein the nanopore sequencing device comprises an electrolytic cell provided with an anode and a cathode, and a solid nanopore film for separating the anode from the cathode of the electrolytic cell; placing the nucleic acid molecules to be measured in a cathode cavity of the electrolytic cell,; and when the nucleic acid molecules are measured, applying the voltage between the anode and the cathode, and introducing a reverse external field taking a pressure intensity external field as an electric field simultaneously. According to the method, the pressure intensity external field is reversely applied under the drive of an additional electric field, so that the speed of DNA (Deoxyribonucleic Acid) molecules is reduced when the DNA molecules pass through the solid nanopore, and the speed is reduced by about 50% to 80%, thus the time resolution of a DNA unimolecule detection technology is greatly improved. The method has very bright application prospect on a solid nanopore DNA molecule sequencing device.

Description

technical field [0001] The invention relates to a method for decelerating nucleic acid molecules in a solid nanopore. Background technique [0002] DNA single-molecule detection and analysis based on solid-state nanopore devices is considered to be one of the most promising technical routes for realizing the third generation of rapid and low-cost human gene sequencing (realizing the genome sequencing of a single person within 24 hours at a cost of less than $1,000), and has become In addition to research groups such as Harvard University, Boston University, Brown University, Caltech, and Delft University of Technology in the Netherlands, IBM also announced in October 2009 to join the next generation of human genome sequencing- The $1,000 plan uses nanopore device-based sequencing technology as a realization method, enabling nanopore research to move from basic research to application (D.Branton, et al.Nature Biotechnology26(10), 1146(2008); M.Zwolak and M. Di Ventra, Review...

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Problems solved by technology

Moreover, with the increase of glycerol, the viscosity coefficient of the solution increases, and the amplitude of the DNA perforation blocking current value decreases, so that the signal-to-noise ratio of the test decreases, resulting in an increase in the measurement error

In addition, the DNA perforation speed can be slowed down by lowering the voltage, but the lowering of the voltage leads to a lower current signal and a worse signal-to-noise ratio, making measurement very difficult

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