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Sensitive membrane for electrochemical biosensors and preparation method thereof

A technology of biosensors and sensitive membranes, applied in the field of electrochemical biosensors and its preparation, to achieve the effects of improving response sensitivity, simple preparation process, and improving selectivity

Inactive Publication Date: 2012-07-18
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the doping of these electron transporters and electron mediators into silica sol-gels to improve the sensitivity and selectivity of electrochemical biosensors has not been reported.

Method used

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  • Sensitive membrane for electrochemical biosensors and preparation method thereof
  • Sensitive membrane for electrochemical biosensors and preparation method thereof
  • Sensitive membrane for electrochemical biosensors and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A. Preparation of chitosan-silica sol doped with Prussian blue modified multi-walled carbon nanotubes

[0060] A-1. According to the final concentration of multi-walled carbon nanotubes of 0.5mg / mL, add them to the mixed acid of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1, sonicate for 6 hours at room temperature, and collect carbon nanotubes by centrifugation at 14000 rpm Wash it with deionized water to neutrality, and then dry it in a vacuum oven at 50°C for 12 hours;

[0061] A-2. Ultrasonically disperse the acid-treated multi-walled carbon nanotubes in deionized water with a pH of 1.5 for 0.5 h, so that the final concentration of carbon nanotubes is 0.5 mg / mL, and then add ferric chloride and potassium ferricyanide and potassium chloride to make the final concentrations of 1.2, 2.6, and 11.0 mg / mL, respectively, and then stirred at room temperature for 24 hours to collect the multi-walled nanotube complexes and wash them to neu...

Embodiment 2

[0069] A. Preparation of chitosan-silica sol doped with Prussian blue modified multi-walled carbon nanotubes

[0070] A-1. According to the final concentration of multi-walled carbon nanotubes of 0.5mg / mL, add them to the mixed acid of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1, sonicate for 6 hours at room temperature, and collect carbon nanotubes by centrifugation at 14000 rpm Wash it with deionized water to neutrality, and then dry it in a vacuum oven at 50°C for 12 hours;

[0071] A-2. Ultrasonically disperse the acid-treated multi-walled carbon nanotubes in deionized water with a pH of 1.5 for 0.5 h, so that the final concentration of carbon nanotubes is 0.5 mg / mL, and then add ferric chloride and potassium ferricyanide and potassium chloride to make the final concentrations of 1.2, 2.6, and 11.0 mg / mL, respectively, and then stirred at room temperature for 24 hours to collect the multi-walled nanotube complexes and wash them to neu...

Embodiment 3

[0079] A. Preparation of chitosan-silica sol doped with Prussian blue modified multi-walled carbon nanotubes

[0080] A-1. According to the final concentration of multi-walled carbon nanotubes of 0.5mg / mL, add them to the mixed acid of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1, sonicate for 6 hours at room temperature, and collect carbon nanotubes by centrifugation at 14000 rpm Wash it with deionized water to neutrality, and then dry it in a vacuum oven at 50°C for 12 hours;

[0081] A-2. Ultrasonically disperse the acid-treated multi-walled carbon nanotubes in deionized water with a pH of 1.5 for 0.5 h, so that the final concentration of carbon nanotubes is 0.5 mg / mL, and then add ferric chloride and potassium ferricyanide and potassium chloride to make the final concentrations of 1.2, 2.6, and 11.0 mg / mL, respectively, and then stirred at room temperature for 24 hours to collect the multi-walled nanotube complexes and wash them to neu...

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Abstract

The invention relates to a chitosan-silica sol-gel sensitive membrane for electrochemical biosensors and a preparation method thereof, and the sensitive membrane is covalently bonded with bioactive substances and doped with electron carriers and electron mediators. By means of the sol-gel process of silane coupling agent and the covalent coupling of the silane coupling agent, the bioactive substances and chitosan, the bioactive substances are covalently bonded with a synchronously formed chitosan-silica sol-gel three-dimensional mesh structure. Meanwhile, the electron carriers and the electron mediators are doped in the chitosan-silica sol-gel three-dimensional mesh structure. Compared with the prior art, the sensitive membrane for electrochemical biosensors, which is provided by the invention, can remarkably enhance the stability, selectivity and sensitivity of the sensors.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors and preparation thereof, and particularly designs a chitosan-silica sol-gel electrochemical biosensor covalently bonded with biologically active substances and doped with electron mediators and electron mediators Sensitive film and its preparation method. Background technique [0002] Electrochemical biosensors are widely used in clinical diagnosis, food and drug analysis, and environmental control due to their advantages of high sensitivity, high selectivity, low cost and simple operation. The application of electrochemical biosensors in practical analysis and detection also brings considerable economic benefits. The preparation of sensitive membranes for electrochemical biosensors is a key step in the construction of electrochemical biosensors. [0003] Chitosan-silica sol-gel is widely used as a sensitive membrane for electrochemical biosensors due to its excellent biocompa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 戴志飞付光磊岳秀丽
Owner HARBIN INST OF TECH
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