Method for preparing high-purity baicalin

Abstract

The invention relates to a simple and effective method for preparing high-purity baicalin, and relates to an extraction method of medicinal herb. The method comprises the steps of: extracting the medicinal herb with aqueous solution to obtain extract, adsorbing the extract with screened macroporous adsorbent resin, eluting with 0-30% ethanol water solution to remove impurities, then eluting with 40-60% ethanol water solution, concentrating the eluate under reduced pressure, then carrying out freeze drying to obtain a crude product with baicalin content more than 80%, and recrystallizing the crude product with ethanol / water / acetic acid solvent system to obtain the light yellow product, the purity of which is more than 98% detected by high-performance liquid chromatography. The method provided by the invention is simple in process, low in cost, mild in condition and environment-friendly, and is more suitable for industrial production of high-purity baicalin.

Description

technical field [0001] What this patent relates to is a kind of preparation method that can be used as the raw material medicine of pharmaceutical industry, what specifically relate to is the preparation method of high-purity baicalin (Baicalin). Background technique [0002] Scutellaria baicalensis is the dry root of Scutellaria baicalensis Georgi, a plant in the Labiatae family. It is a commonly used traditional Chinese medicine. Clinically, it is widely used in diseases such as pulmonary fever, hemoptysis, enteritis and dysentery, jaundice, hypertension and suppurative infection. Baicalin is the main medicinal component of Scutellaria baicalensis, which has a variety of significant biological activities, including anti-inflammatory, anti-viral, anti-tumor, chemoprevention, etc. As a medicine, baicalin is clinically used for diseases such as pneumonia, hepatitis, and infection, such as baicalin tablets, baicalin capsules, etc. The structural formula of baicalin is as fol...

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Problems solved by technology

[0004] In recent years, literature reports mainly contain high-speed countercurrent chromatography (Haitao Lu, Yue Jiang.Feng Chen.Journal of Chromatography A, 1017(2003): 117-123; Sujuan Wu, Ailing Sun, Renmin Liu.Journal ofChromatographyA, 1066 (2005): 243-247.), the shortcoming of this method is to require special instrument, and preparation process is loaded down with trivial details, and the time spent of preparing a large amount of target products is longer; Next also has the method of solvent extraction (Huiru Dong, Pengyu Bi, Shihui Wang.Analytical Letters, 38(2005): 257-270; Emika Ohkoshi, Tomomi Nagashima, et al.Journal of Chromatography A, 1216(2009): 2192-2194.), its shortcoming is that a large amount of Organic solvents can cause serious pollution to the environment; literature has also reported that the main component (Mei-Chih Lin, Ming-Jer Tsai, Kuo-Ching Wen.Journal of Chromatography A, 830( 1999): 387-395.), but the instruments involved in this method are expensive, which restricts its application, and the second is that the restrictor tube of the instrument is easy to block. When the sample moisture is too large or the viscosity of some components in the extract is too high, it is easy to Capillary blockage, which can lead to the scrapping of the restrictor tube in severe cases; the preparation method of the scutellaria baicalensis extract recorded in the first part of the "Pharmacopoeia of the People's Republic of China" 2010 edition is currently a relatively effective method for obtaining crude baicalin, but the process involves strong acid Strong alkali will have a certain impact on the structural stability of baicalin, and a large amount of acid-base waste liquid will be produced in the production process, and the content of baicalin is only about 80% (Chinese Pharmacopoeia Commission, "Pharmacopoeia of the People's Republic of China", 2010 Edition 2, China Medical Science and Technology Press, Beijing, 2010: 282.)

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