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Method for preparing nanoparticle oil adjuvant vaccine

An oil adjuvant and nanoparticle technology, which is applied in the field of preparation of nanoparticle oil adjuvant vaccines, can solve problems such as short-lived immunity, and achieve the effects of large drug loading, high encapsulation efficiency and low viscosity

Inactive Publication Date: 2012-07-18
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the immunity provided by the inactivated vaccine is relatively short-lived. In order to complete the immunization operation, multiple vaccinations are required.

Method used

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  • Method for preparing nanoparticle oil adjuvant vaccine
  • Method for preparing nanoparticle oil adjuvant vaccine
  • Method for preparing nanoparticle oil adjuvant vaccine

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Experimental program
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specific Embodiment approach 1

[0010] Specific embodiment one: the preparation method of the nanoparticle oil adjuvant vaccine of the present embodiment, carry out according to the following steps: one, get 5mL concentration be the chitosan solution of 0.5~2mg / mL or 5mL concentration be the N of 0.5~2mg / mL -2-Hydroxypropyltrimethylammonium chloride chitosan solution, drop 1.25-7.5mL Newcastle disease L-series virus solution into it, stir evenly to obtain solution A; 2. Put solution A at room temperature, sterile Under the condition of 900~1300r / min magnetic stirring for 30s, then dropwise add 2.5mL sodium tripolyphosphate with a concentration of 0.5~3mg / mL to solution A, stir evenly, add 1mL PBS buffer solution with pH value 7.4 dropwise at a constant speed, stir After uniformity, add 1mL of Span-80 dropwise at a constant speed to obtain solution B; 3. Centrifuge solution B at 4°C and 13000r / min for 30min, absorb the supernatant, and obtain solution B 1 , the precipitate was suspended in deionized water, an...

specific Embodiment approach 2

[0012] Specific embodiment two: this embodiment is different from specific embodiment one: the preparation method of chitosan solution in step one is: take chitosan and disperse it in sterilized deionized water, under magnetic stirring, use mass Dissolve in 1% acetic acid, filter through a 0.22 μm filter, and let it stand until the air bubbles disappear. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0013] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the preparation method of N-2-hydroxypropyltrimethylammonium chloride chitosan solution in step one is: take N-2-hydroxypropyl Trimethylammonium chloride chitosan was dispersed in sterilized deionized water, dissolved with 1% acetic acid under magnetic stirring, filtered with a 0.22 μm filter, and allowed to stand until bubbles disappeared. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for preparing a nanoparticle oil adjuvant vaccine. The method comprises the following steps of: adding newcastle disease virus liquid into a chitosan solution or an N-2-hydroxypropyl trimethyl ammonium chloride chitosan solution to obtain a solution A; adding sodium tripolyphosphate and a phosphate buffer solution (PBS) into the solution A, stirring, adding span-80 to obtain a solution B; centrifuging the solution B, extracting supernate to obtain a solution B1, suspending precipitation by using deionized water, and performing suspension to obtain a solution B2; sterilizing white oil for injection at high temperature, adding the span-80 and cooling to obtain a solution C1; adding tween-80 into the solution B1 to obtain a solution C2; adding the solution C2 into the solution C1, and stirring to obtain a solution C; and adding the solution B2 into the solution C, and stirring to obtain the nanoparticle oil adjuvant vaccine. The nanoparticle oil adjuvant vaccine is small in particle sizes of virus-loaded nanoparticles, high in envelop rate and large in medicine-carrying quantity, and the method has a mild preparation condition and a simple preparation process, and is low in production cost.

Description

technical field [0001] The invention relates to a preparation method of a nanoparticle oil adjuvant vaccine. Background technique [0002] Newcastle disease is an acute, highly contagious infectious disease caused by Newcastle disease virus. Now almost all countries in the world have reports of the prevalence of this disease, which seriously endangers and threatens the poultry industry in the world. It is recognized by the World Organization for Animal Health ( OIE) is listed as a class A infectious disease. Vaccination is currently adopted as the main measure to control Newcastle disease in most countries. The manufacturing process of inactivated vaccine is simple, the immunogenicity is good, and it is easy to manufacture multivalent vaccine. The method of use is usually by injection. After entering the body, it can directly cause an immune response, but it cannot grow and reproduce, so it is relatively safe and stable. However, the immunity provided by the inactivated v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/17A61P31/14
Inventor 赵凯陈刚
Owner HEILONGJIANG UNIV
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