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Method for detecting heavy metals in carbohydrate agricultural products and application thereof

A technology for carbohydrates and agricultural products, which is applied in the field of detection of heavy metals in carbohydrate agricultural products. It can solve problems such as laborious, time-consuming, and harmful gas generation, and achieve the effects of speeding up time, mild reaction conditions, and reducing environmental pollution.

Active Publication Date: 2012-07-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these pretreatment techniques can accurately extract the heavy metals in the sample, they are time-consuming, laborious and produce a large amount of harmful gases. alkaline), so the digestion solution obtained by the traditional sample pretreatment method cannot be directly applied to the detection of heavy metals by ELISA

Method used

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  • Method for detecting heavy metals in carbohydrate agricultural products and application thereof
  • Method for detecting heavy metals in carbohydrate agricultural products and application thereof
  • Method for detecting heavy metals in carbohydrate agricultural products and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Sample preparation:

[0053] First rinse the samples (vegetables, fruits) with tap water, then rinse with ultrapure water for 3 times, and dry the water. Homogenize with a food homogenizer, store in a 250ml beaker, and freeze at -4°C for later use.

[0054] Grain samples are directly dry-ground to powder and stored in a sealed box at room temperature for later use.

[0055] (2) Pretreatment of samples (dilute acid leaching samples)

[0056] ①Vegetable agricultural products

[0057] A. Weigh 2.0g of Chinese cabbage prepared in step (1), place it in a 10ml centrifuge tube, add 2.0ml of 2.0M HCl solution, react at room temperature for 20min, centrifuge at 4000rpm for 15min, and take the supernatant In a 2ml centrifuge tube, centrifuge at 13000rpm for 10min, take the supernatant and adjust the pH value to 6.0-8.0, set the volume to 4ml, and measure the concentration of heavy metals copper, lead, and cadmium by GF-AAS (graphite furnace atomic spectrophotometer). conten...

Embodiment 2

[0080] The precision of the pretreatment step of sample in the inventive method:

[0081] (1) Weigh multiple groups of samples, after microwave digestion according to the national standard method, use GF-AAS to measure the content of heavy metal copper, lead, and cadmium in each group of samples, and take the average value to obtain the heavy metal content of the sample before adding the standard, as follows :

[0082] ① Microwave digestion of vegetable agricultural products:

[0083] Use an electronic balance to weigh 3 samples of Chinese cabbage, carrot, and pumpkin, each about 2.000g, put them into a polytetrafluoroethylene digestion tank, and add 5ml of high-grade pure concentrated HNO 3 , 2ml of 30% H 2 o 2 , placed at room temperature for 8 to 12 hours, and then placed in a microwave digestion apparatus for digestion. The digestion conditions are shown in Table 3.

[0084] Table 3 Microwave digestion conditions of vegetable samples

[0085]

[0086] After the diges...

Embodiment 3

[0114] Utilize the inventive method to detect the heavy metal of carbohydrate agricultural product:

[0115] (1) Coating: Dissolve Cu-DTPA-OVA with 0.05M pH9.6 carbonate buffer (for details, see: Preparation of heavy metal copper ion monoclonal antibody and establishment of indirect competitive ELISA detection method[J]. China Bio Journal of Product Science, 2011, 24(6): 720-723.), the concentration is 160ng / ml, 100μl per well, and coated on the microwells of the microplate at 4°C for 16h.

[0116] (2) Dry the liquid in the wells of the plate, then add PBS-T (0.015M, pH 7.4, containing 0.05% Tween-20) washing solution to the wells, 300μl per well, let stand for 3min, shake gently, discard the washing solution , pat dry, and repeat washing the plate three times;

[0117] (3) Blocking: block with PBST buffer containing 0.05 g / ml skimmed milk powder, 200 μl per well, 37° C. for 1 hour; wash the plate three times as before.

[0118] (4) Adding samples: Add Cu at a concentration ...

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Abstract

The invention discloses a method for detecting heavy metals in carbohydrate agricultural products and application thereof. The method of the invention comprises the following concrete steps: homogenizing a carbohydrate agricultural product, and leaching with a diluted acid; removing insoluble substances, centrifuging, and collecting supernatant to obtain a solution containing heave metal; and directly using the to-be-tested sample solution to enzyme linked immunosorbent assay, and obtaining heavy metal content in the to-be-tested sample. The method of the invention can be used for leaching heavy metal ions copper, lead and cadmium in vegetables, fruits and foodstuff agricultural products, which not only quickens the time of leaching heave metal from a sample, but also reduces environmental pollution brought by the conventional sample pretreatment technology. The method has mild reaction conditions, and achieves rapid, efficient and convenient field detection of agricultural products.

Description

technical field [0001] The invention relates to a method for extracting heavy metals, in particular to a method and application for detecting heavy metals in carbohydrate agricultural products. This method is a simple, efficient and accurate detection method. Background technique [0002] In recent years, my country's economy has developed rapidly, but at the same time, the environmental pollution caused has become more and more obvious, especially the heavy metal pollution of the atmosphere, water and soil caused by industry and processing and manufacturing. Heavy metal pollution in water, soil and farmland is the most important cause of excessive heavy metal content in agricultural products such as vegetables, fruits and grains. [0003] Heavy metals copper, lead, and cadmium mainly enter the human body through food and breathing. Although heavy metals in agricultural products generally do not cause acute poisoning to people, heavy metals accumulate in the human body thr...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N1/28
Inventor 江天久李支微唐勇邹军辉江涛
Owner JINAN UNIVERSITY
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