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Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder

A technology of freeze-drying bacterial powder and cholesterol, which is applied in food preparation, dairy products, milk preparations, etc.

Active Publication Date: 2012-07-04
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no relevant domestic and foreign literature reports and patent reports on the method of preparing freeze-dried bacterial powder by using Kluyveromyces marxe for reducing serum cholesterol

Method used

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  • Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder
  • Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder
  • Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder

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Effect test

Embodiment 1

[0026] Example 1. Preparation method of serum cholesterol-lowering Kluyveromyces marxianus K1 and M3 freeze-dried bacterial powder

[0027] 1. Activation

[0028] K1 and M3 of Kluyveromyces marxianus preserved in the General Microbiology Center of China Microbial Species Collection Management Committee were respectively inoculated into 10 mL of potato lactose liquid medium and cultured at 28°C for 21 hours. After subculturing 3 times in this way, the activated bacterial culture medium can be obtained, and the number of viable bacteria can reach 10 7 cfu / mL or more.

[0029] 2. Expand training

[0030] The above 0.5 mL activated strains K1 and M3 culture solutions were respectively inoculated into 50 mL potato lactose liquid medium, and cultured at 28° C. for 21 hours to obtain an expanded culture solution.

[0031] 3. Fermentation

[0032] Inoculate 20-30 mL of the above-mentioned strains K1 and M3 expanded culture solution respectively in 1000 mL modified potato lactose liquid medium, ...

Embodiment 2

[0043] Example 2: Animal experiment of Kluyveromyces marxianus K1 and M3 freeze-dried bacterial powder for lowering serum cholesterol

[0044] 1. Animal grouping and handling

[0045] Forty Wistar rats (provided by Beijing Weitong Lihua Experimental Animal Technology Company) were randomly divided into 4 groups, namely high-fat model group, low-dose group, medium-dose group, and high-dose group. All groups were fed high-fat feed (basic feed 78.8%, cholesterol 1%, egg yolk powder 10%, lard 10%, bile salt 0.2%, all provided by Beijing Weitong Lihua Laboratory Animal Technology Company), adaptive feeding 1 week. After 1 week, all groups continued to feed the high-fat diet, and the low-dose group, medium-dose group, and high-dose group were given 0.5g / kg·d, 1.5g / kg·d and 2.5g / kg·d respectively. Kluyveromyces marxianus K1 and M3 mixed freeze-dried bacterial powder (the mixing ratio of K1 and M3 freeze-dried bacterial powder is 1:1, and the number of viable bacteria reaches 10 9 cfu / g ...

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Abstract

The invention relates to a preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder. The preparation method is applicable to the production of serum cholesterol reduction functional probiotic preparations and fermentation dairy products. The two serum cholesterol reduction strains of Kluyveromyces marxianus K1 and M3 screened from Tibetan kefir are utilized, and the freeze-dried powder is prepared through activation and amplification culture, fermentation, centrifugal concentration, freeze-dried protection agent addition, pre-freezing and freeze drying. A free-dried protection agent composition recipe is optimized, so the strain survival rate is higher than 65 percent, and in addition, the viable count of the free-dried powder is higher than 109cfu / g. The serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder prepared by the method has various probiotic effects of reducing the serum cholesterol, improving the organism antioxidation, promoting the intestinal tract flora balance and the like.

Description

Technical field [0001] The invention relates to a preparation method of Kluyveromyces marxianus freeze-dried bacteria powder for lowering serum cholesterol, which is suitable for the production of serum cholesterol lowering functional probiotic preparations and fermented dairy products. Background technique [0002] Probiotics refer to a type of microorganisms that improve the microecological balance of specific parts of the host through intestinal colonization and have several other beneficial physiological functions. They can improve the ecological balance of intestinal microbes, control intestinal infections, reduce serum cholesterol levels, increase the utilization of lactose by lactose intolerant patients, and can stimulate specific or non-specific immune responses and have an immune-enhancing effect. The live bacteria of probiotic products should be metabolically stable and active, survive in large numbers after passing through the digestive tract, and play the role of prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A23C9/12
Inventor 刘慧谢远红张红星贾慧钟思琼熊利霞高秀芝
Owner BEIJING UNIV OF AGRI
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