Method for quantitative detection of zearalenone

A technology of zearalenone and colloidal gold, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable conventional detection and use, and achieve the effects of easy promotion and application at the grassroots level, fast detection speed, and single detection object

Inactive Publication Date: 2012-06-27
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

However, these methods require strict pretreatment of test samples, expensive instruments such as high performance liquid chromatography, and professional operators, which are not conducive to on-site routine testing.

Method used

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  • Method for quantitative detection of zearalenone
  • Method for quantitative detection of zearalenone
  • Method for quantitative detection of zearalenone

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Experimental program
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Embodiment

[0031] 1. Antigen preparation

[0032] (1) Preparation of zearalenone-coated antigen

[0033]Take 0.33ml (3mg / ml) ZEN, mix it in 1.2ml pyridine, add 2mg O-carboxymethyl hydroxylamine, stir and react at room temperature for 24h; after vacuum drying the solution, add 4ml distilled water and dissolve it, adjust the pH to 8.0 ; The unreacted ZEN was extracted and removed with benzene (3ml benzene, extracted 3 times in total), the benzene phase was removed, and the water phase was retained. The pH of the aqueous phase was adjusted to 3.0, extracted with ethyl acetate (10 ml ethyl acetate, extracted 4 times in total), the ester phase was extracted, and the aqueous phase was discarded. The ester phase was filtered with anhydrous sodium sulfate and dried. The dried crystals were dissolved in 0.5ml basic alumina-treated dioxane; 20mgBSA was weighed and dissolved in 0.7ml 0.05mol / L (pH7.2) of PBS; after that, the two solutions were dissolved in 4 Mix slowly at ℃ to obtain solution a;...

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Abstract

The invention relates to a method for quantitative detection of zearalenone. The method comprises the following steps of: 1. preparing conjugates of ZEN-BSA and ZEN-OVA; 2. preparing an anti-ZEN monoclonal antibody; 3. preparing colloidal gold and marking the anti-ZEN monoclonal antibody; and spraying the marked monoclonal antibody on a colloidal-gold pad; 4. carrying out sample application on a nitrocellulose membrane; 5. assembling into a test strip; 6. drafting a standard curve of ZEN concentration and chromogenic value, calculating a chromogenic value of a sample to be measured into the standard curve to obtain a ZEN content in the sample to be measured. The method of the invention has advantages of single detection object, strong pertinency, high detection speed and short time; and untrained personnel can carry out detection by the method, so as to satisfy requirement of rapid and accurate determination of zearalenone content by inspection departments like grain storage and sale organization, exit and entry department and the customs and facilitate primary popularization and application.

Description

technical field [0001] The invention relates to a method in the technical field of biological detection, in particular to a method for quantitatively detecting zearalenone. Background technique [0002] Zearalenone toxin (Zearalenone, ZEN) is a secondary metabolite produced by Fusarium fungus under certain humidity and temperature conditions. Zearalenone toxin can exist in corn, wheat, barley, sorghum, rye and other grains, and can also exist in animal tissues that eat ZEN-containing feed, including milk and eggs. Zearalenone toxin is associated with spontaneous breast cancer, oviduct and uterine edema, hyperplasia, sperm cell aberration, apoptosis and other diseases. Zearalenone toxin has the characteristics of widespread distribution, long residual time, difficult to handle, and enhanced toxicity with other toxins. After joining the WTO, the international trade volume of agricultural products and related foods has increased day by day, and the requirements for the biosaf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 严亚贤王元凯孙建和
Owner SHANGHAI JIAO TONG UNIV
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