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Method for preparing bioluminescent nano thermometer

A fluorescent nanometer and thermometer technology, which is applied in the field of nanomaterials, can solve the problems that cannot be realized, and achieve the effect of small size, good biocompatibility and low toxicity

Inactive Publication Date: 2012-06-27
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although traditional methods such as mercury thermometers, bimetallic thermometers, thermocouples, and fiber optic temperature sensors have been successfully used in various industrial fields, they are often unable to realize the principle of biological environments such as cells smaller than 10 microns. bit probing

Method used

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  • Method for preparing bioluminescent nano thermometer
  • Method for preparing bioluminescent nano thermometer
  • Method for preparing bioluminescent nano thermometer

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preparation example Construction

[0022] The preparation method of the bioluminescent nanometer thermometer of the present invention is specifically:

[0023] 1.) Use N-isopropylacrylamide (NIPAM) as a monomer, methylenebisacrylamide (N,N-methylene-bis-acrylamide, BIS) as a crosslinking agent, potassium persulfate ( Potassium persulfate, KPS) was used as the initiator, and Rhodamine 6G (Rhodamine 6G, R6G) was used as the fluorescent active molecule, and the molar ratio of the four was 700:50:15:1~3000:150:40:1. First, NIPAM and BIS Add the mixed aqueous solution of the three-necked flask, then add R6G and mix evenly, and finally add the KPS aqueous solution in the anaerobic environment where the argon gas is continuously passed through the three-necked flask, react in a water bath at 60~80°C for 2~8 hours, and then wash it by centrifugation , remove the supernatant, disperse the remaining product in deionized water, and prepare fluorescent thermosensitive polymer microspheres;

[0024] 2.) Mix the fluorescent...

Embodiment 1

[0028] 1) Preparation of fluorescent thermosensitive polymer microspheres:

[0029] Take 340mg NIPAM and 23mg BIS to make 20ml aqueous solution and put it into a 50ml three-neck flask, then add 0.1ml (0.01mol / L) R6G solution, stir magnetically, and after argon gas for half an hour, quickly add 4ml (0.01mol / L) L) KPS aqueous solution, after reacting in a water bath at 80°C for about 8 hours, centrifuge and wash, remove the supernatant, and disperse the remaining product into 10 ml of deionized water to obtain fluorescent thermosensitive polymer microspheres.

[0030] 2) Preparation of bioluminescence nanothermometer:

[0031] Under the condition of stirring at room temperature, take 2ml of the temperature-sensitive microspheres prepared in the above step 1) into a 30ml three-necked flask, dilute with 8ml of deionized water, add 6ml (0.01mol / L) silver nitrate solution, stir evenly with argon, and deoxygenate with argon , after half an hour, quickly add 1ml of fully cooled (0.01...

Embodiment 2

[0037] 1) Preparation of thermosensitive polymer microspheres:

[0038] Take 80 mg NIPAM and 8 mg BIS to make 20 ml aqueous solution and add it to a 50 ml three-neck flask, then add 0.1 ml (0.01mol / L) R6G solution, stir magnetically, and after argon gas for half an hour, quickly add 1.5 ml (0.01mol / L) / L) KPS aqueous solution, reacted in a water bath at 60°C for about 2 hours, centrifuged and washed, removed the supernatant, and dispersed the remaining product into 10 ml deionized water to obtain fluorescent thermosensitive polymer microspheres.

[0039] 2) Preparation of bioluminescence nanothermometer:

[0040] Under the condition of stirring at room temperature, take 2ml of the fluorescent temperature-sensitive microspheres prepared in the above step 1) and add them to a 30ml three-neck flask, dilute with 8ml of deionized water, add 1ml (0.01mol / L) silver nitrate solution, stir evenly with argon, and deoxygenate with argon After half an hour, quickly add 1ml of sodium boro...

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Abstract

The invention discloses a method for preparing a bioluminescent nano thermometer. The prepared bioluminescent nano thermometer is characterized in that: on the one hand, the fluorescence intensity is controlled through temperature by using a fluorescence quenching effect; at a low temperature, because the thermo-sensitive polymer is in a stretching state, the distance between fluorescent active molecules and metal silver nano spheres is prolonged, the fluorescence quenching effect is weak, and fluorescent signals are strong; when the temperature is raised, the thermo-sensitive polymer is shrunk, so that the distance between the fluorescent active molecules and the metal silver nano spheres is shortened, the fluorescence quenching effect is enhanced, and the fluorescent signals are weakened; and on the other hand, the maximum fluorescence intensity of the bioluminescent nano thermometer is linearly changed together with the environmental temperature, the fluorescence intensity is sensitive together with temperature change, and the maximum fluorescence intensity and the fluorescence intensity can be quantitatively and correspondingly valued, so that the prepared bioluminescent nano thermometer can be used as a nano thermometer.

Description

technical field [0001] The invention relates to the field of nanomaterials and the technical field of biological temperature sensors, in particular to a method for preparing a bioluminescent nanometer thermometer based on nanospheres with temperature-sensitive fluorescent properties. Background technique [0002] Poly N-isopropylacrylamide (poly N-isopropylacrylamide, pNIPAM ) is a temperature-responsive polymer that swells at low temperatures and shrinks at high temperatures. The special temperature-sensitive properties of pNIPAM make it widely researched in the fields of drug delivery, temperature-sensitive switch and controlled release of drugs. On the other hand, in recent years, metal nanoparticles have also been rapidly developed due to their excellent optical, electrical, and magnetic properties, and they are playing an increasingly important role in the fields of photocatalysis, light sensing, and medical diagnosis and treatment. Therefore, by combining pNIPAM with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L33/26C09K11/06C09K11/02C08F220/56C08F222/38C08K3/08C08J3/03G01K11/32A61K47/32
Inventor 王著元崔一平邵平宗慎飞
Owner SOUTHEAST UNIV
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