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Silk fibroin multilayer functional membrane with gradient structure and its preparation method

A silk fibroin, gradient structure technology, applied in the field of biomedical materials, to achieve the effect of accelerating bone regeneration rate, improving biological properties, and enhancing osteoconductive and osteoinductive capabilities

Active Publication Date: 2012-06-27
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shao Zhengzhong et al. (a full silk fibroin composite material and its preparation method, Chinese invention patent, application number: 200710171825) used degummed cocoon silk in oriented arrangement as a reinforcing layer, and then cast it with a high-concentration silk fibroin aqueous solution. Membrane approach, obtained a high-performance all-silk fibroin composite, but still a single-layer dense membrane

Method used

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  • Silk fibroin multilayer functional membrane with gradient structure and its preparation method
  • Silk fibroin multilayer functional membrane with gradient structure and its preparation method
  • Silk fibroin multilayer functional membrane with gradient structure and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 0.05g of silk fibroin fabric, the concentration of immersion is 0.167mol L -1 , Soak it in the calcium source solution at 37°C for 0.5h, take it out, wash it twice with distilled water, and then immerse it at a concentration of 0.100mol L -1 in the phosphorus source solution for 0.5h. After repeated alternate dipping in this way for 6 times, the calcium phosphate-silk fibroin textile composite membrane (intermediate layer) was obtained after being washed with distilled water and ethanol for 3 times respectively, and dried at room temperature. from figure 2 In the SEM photo of the middle layer shown, it can be seen that calcium phosphate is deposited on the silk fibroin.

[0026] 0.3gPVP is added to the silk fibroin solution that 20ml concentration is 50g / L to form PVP-silk fibroin mixed solution (wherein the mass percentage of PVP and silk fibroin is 30%), this mixed solution is cast on the surface of the middle layer After drying at 10° C. to form a film, put ...

Embodiment 2

[0030] Take 0.10g of silk fibroin textile fabric, the concentration of immersion is 0.100mol L -1 , soaked in the calcium source solution at a temperature of 50°C for 2 hours, took it out, soaked it with distilled water for 3 times, and then immersed it in a concentration of 0.150mol L -1 , In the phosphorus source solution at a temperature of 50°C for 2h. Repeated alternate dipping in this way for 15 times, washed with distilled water and ethanol three times respectively, and dried at room temperature to obtain the calcium phosphate-silk fibroin textile composite membrane (middle layer).

[0031] 0.5g PEG is added to the silk fibroin solution that 20ml concentration is 100g / L to form PEG-silk fibroin mixed solution (the mass percentage of PEG and silk fibroin is 25%), and this mixed solution is cast on the surface of the middle layer After drying at 50° C. to form a film, put it into a vacuum drying oven to dry for 48 hours to obtain a double-layer film including an intermed...

Embodiment 3

[0035] Take 0.50g of silk fibroin textile fabric, the concentration of immersion is 0.800mol L -1 , Soak in calcium source solution at 20°C for 3 hours, take it out, soak it with distilled water for 3 times, and then immerse it in a concentration of 0.600mol L -1 , The temperature is 20 ℃ in the phosphorus source solution for 2h. After repeated alternate dipping in this way for 50 times, the calcium phosphate-silk fibroin textile composite membrane (intermediate layer) was obtained after being washed three times with distilled water and ethanol respectively, and then dried at room temperature.

[0036] 0.16g PVA is added to the silk fibroin solution that 20ml concentration is 80g / L to form PVA-silk fibroin mixed solution (the mass percentage of PEG and silk fibroin is 10%), and this mixed solution is cast on the surface of the middle layer After drying at 40° C. to form a film, it was placed in a vacuum drying oven for 64 hours to obtain a double-layer film containing an inte...

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Abstract

The invention discloses a silk fibroin multilayer functional membrane with a gradient structure and its preparation method. The method comprises: adopting a silk fibroin membrane with nanopores as a barrier layer, controlling the crystalline form and pore structure of silk fibroin, adjusting the barrier effect of silk fibroin on the connective tissues and its effect on nutrient exchange; using a silk fibroin textile / calcium phosphate composite material of high strength as an intermediate layer to provide mechanical strength and dimensional stability for a guided bone regeneration membrane; utilizing silk fibroin to construct a porous layer, then adjusting the degradation behavior of the porous layer, the cellular affinity, and providing a growing space for extracellular matrixes and bone regeneration. The method employs crosslinking treatment to increase the integrity between different layers. With good bone regeneration capability, the multilayer functional membrane of the invention has a degradation rate and mechanical strength that match with the bone regeneration rate.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a silk fibroin multilayer functional film with a gradient structure and a preparation method thereof, which are applied to guided bone regeneration technology. Background technique [0002] Tissue regeneration technology has become the research frontier in the field of biomedicine. The extracellular matrix plays a role in supporting tissue stability, protecting cells, and supporting biological factors that stimulate and stimulate cell functions during the regeneration process. It can be seen that the design and construction of extracellular matrix is ​​the premise and key to the completion of tissue regeneration technology. Not only that, the site and form of interaction between extracellular matrix and cells also determine the growth behavior of cells and the function of tissues. Due to the relatively simple structure and function of bone, bone regeneration techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12A61L31/14C08J9/40C08J7/04C08J7/02
Inventor 李延报江芸程新陆春华许仲梓
Owner NANJING TECH UNIV
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