Detecting method for effective components of traditional Chinese medicine Hugu capsules for preventing and treating osteoporosis
A technology for active ingredients and osteoporosis, applied in the field of detection of active ingredients, can solve problems such as cumbersome operation, incomplete active ingredients, and inability to meet quality control
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Embodiment 1
[0043] (1) Instruments and reagents.
[0044] Instrument: Agilent1200 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); Kromasil100-5C18 chromatographic column; Sartorius one hundred thousandth balance; KQ5200DB numerical control Ultrasonic cleaner; Milli-pore-Q ultrapure water preparation instrument.
[0045] Reference substances: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid, naringin.
[0046] Sample to be inspected: bone-protecting capsule.
[0047] Other reagents: acetonitrile (chromatographically pure), methanol, glacial acetic acid (analytical pure).
[0048] (2) Detection method.
[0049] a. Preparation of mixed reference substance solution: Accurately weigh 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid , an appropriate amount of naringin, dissolved in 100% methanol, constant volume, shaken, and filtere...
Embodiment 2
[0060] (1) Instruments and reagents.
[0061] Instrument: Agilent1200 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); Kromasil100-5C18 chromatographic column; Sartorius one hundred thousandth balance; KQ5200DB numerical control Ultrasonic cleaner; Milli-pore-Q ultrapure water preparation instrument.
[0062] Reference substances: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid, naringin.
[0063] Sample to be inspected: bone-protecting capsule.
[0064] Other reagents: acetonitrile (chromatographically pure), methanol, glacial acetic acid (analytical pure).
[0065] (2) Detection method.
[0066] a. Preparation of mixed reference substance solution: Accurately weigh 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid , an appropriate amount of naringin, dissolved with 80% ethanol, constant volume, shaken, and filtere...
Embodiment 3
[0077] (1) Instruments and reagents.
[0078] Instrument: Agilent1200 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); Kromasil100-5C18 chromatographic column; Sartorius one hundred thousandth balance; KQ5200DB numerical control Ultrasonic cleaner; Milli-pore-Q ultrapure water preparation instrument.
[0079] Reference substances: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid, naringin.
[0080] Sample to be inspected: bone-protecting capsule.
[0081] Other reagents: acetonitrile (chromatographically pure), methanol, glacial acetic acid (analytical pure).
[0082] (2) Detection method.
[0083] a. Preparation of mixed reference solution: Accurately weigh 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, ferulic acid, icariin, chlorogenic acid , an appropriate amount of naringin, dissolved in 95% ethanol, constant volume, shaken, and filtered through a ...
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