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Method for establishing rat model with IgAN (immunoglobulin A nephropathy) glomerulosclerosis

A technology of glomerulosclerosis and rat model, which is applied in the field of establishment of glomerulosclerosis model, can solve the problem of difficulty in popularization, long time of glomerular fibrosis formation, high price of genetically engineered models and spontaneous models and other issues to achieve the effect of shortening production time, reducing huge medical expenses, and shortening the experimental cycle

Inactive Publication Date: 2012-06-20
SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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Problems solved by technology

[0020] Due to the differences between experimental animals and humans, and the etiology of IgA nephropathy has not yet been definitively answered, so many experimental animal models of IgA nephropathy currently exist are difficult to be completely satisfactory
For example, secondary and mixed models are mixed with many other factors, which cannot simulate the primary process of IgA nephropathy well; genetically engineered models and spontaneous models are too expensive and difficult to promote in China, etc.
At present, only SEB (or SEB-based)-induced models are close to the pathogenesis of human IgA nephropathy, reflecting some of its pathological changes, but the disadvantage of this model is that the formation of glomerular fibrosis takes a long time

Method used

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  • Method for establishing rat model with IgAN (immunoglobulin A nephropathy) glomerulosclerosis

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Co-infection.

[0033] After the SD male rats were adaptively fed for 1 week, they began to give 40mg / ml BSA acidified aqueous solution for intragastric administration every other day, and injected SEB 0.08mg / ml. Adjuvant 0.2ml / only.qw.

[0034] 2. 5 / 6 nephrectomy.

[0035]On the fifth week, 2 / 3 of the left nephrectomy was performed. After anesthesia, the patient was fixed on the operating table in a prone position. The surgical area on the back was routinely disinfected, and a drape was spread. A straight incision on the left side of the back was used to incise the skin, and the psoas major muscle was separated to enter the retroperitoneum. , find the kidney, and use hemostatic forceps to separate the fat capsule and renal pedicle around the kidney, cut the subcutaneous tissue and capsule layer by layer, separate the adrenal gland with special care to avoid damage, fully expose the left kidney, expose the renal pedicle, and use fiber hemostatic forceps Clamp the r...

Embodiment 2

[0039] 1. Establishment of IgAN glomerulosclerosis rat model.

[0040] After 96 SD male rats were fed adaptively for 1 week, they were divided into 4 groups according to the random number table according to their body weight, namely: sham operation group (group A), 5 / 6 nephrectomy group (group B), and combined infection group (group B). Group C), combined infection combined with 5 / 6 nephrectomy group (group D), 24 rats in each group.

[0041] (1). Group A: sham operation group. The same amount of normal saline was given during intragastric administration and intravenous injection, and a sham operation was performed, that is, after intraperitoneal injection of anesthesia in rats, bilateral kidneys were freed, perirenal fat was stripped, and the kidneys were removed. Reset, all the other operations are the same as the 5 / 6 nephrectomy method in the first embodiment.

[0042] (2). Group B: 5 / 6 nephrectomy group. The same amount of normal saline was given during intragastric admin...

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Abstract

The invention discloses a method for establishing a rat model with IgAN (immunoglobulin A nephropathy) glomerulosclerosis, which includes: firstly, performing intragastric administration of bovine serum albumin acidized liquor every other day for a rat fed for one week, and weekly performing penile intravenous injection of staphylococcus aureus enterotoxin B solution and intraperitoneal injection of complete Freund's adjuvant for the rat from the second week to the fourth week; and secondly, performing surgical nephrectomy twice on the fifth week and on the sixth week for the rat treated in the step 1, wherein five sixths of a kidney resected in the two operations. By the method, the time of making the rat model with IgAN glomerulosclerosis is shortened, and occurrence and development of renal tissue fibrosis are enhanced. Therefore, the model can better serve in medical research of IgA nephropathy.

Description

technical field [0001] The invention relates to a method for establishing a glomerulosclerosis model, in particular to a method for establishing a rat model of IgAN glomerulosclerosis. Background technique [0002] IgA nephropathy is glomerulonephritis characterized by IgA deposition in the mesangial region of the glomerulus, some scholars classify it as a systemic disease, but most scholars divide IgA nephropathy into primary and secondary Episodic IgA nephropathy is purpura nephritis, lupus nephritis and hepatitis B / cirrhosis-associated nephritis. IgA nephropathy is the most common primary glomerular disease worldwide. Even in the United States, where the incidence of IgA nephropathy is relatively low, IgA nephropathy still accounts for the first among primary glomerular diseases among adults aged 20-39 bit. Because IgA nephropathy has various clinical manifestations, complex pathological changes and different prognosis, more and more scholars believe that IgA nephropath...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K35/74A61K38/17A61K39/39
Inventor 彭文王云满王浩金周慧刘育军殷佩浩李琦姚卫国付文成朱冰冰程蔚蔚吴芳
Owner SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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