Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for breeding cordyceps militaris by utilizing silkworm larvae

A technology for Cordyceps militaris and larvae, which is applied in the directions of botanical equipment and methods, application, and horticulture, can solve problems such as pollution, waste of resources, immature technology of Cordyceps militaris, and achieves simple technical process, improved success rate, and easy scale. The effect of cultivation

Active Publication Date: 2012-05-09
北京航天农业生物科技有限公司
View PDF7 Cites 39 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are a small number of research reports on the use of silkworm larvae to cultivate Cordyceps militaris, the technology of using silkworm larvae to cultivate Cordyceps militaris is still immature and difficult to promote. The main disadvantages are: the growth period of Cordyceps militaris hosts is 4-5 instars. 6-day-old silkworm larvae, the silk gland protein in silkworm larvae in this growth period is not fully formed, which affects the accumulation of active substances of Cordyceps militaris. Low; in the process of cultivation, cocooning and pupation occur, resulting in waste of resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for breeding cordyceps militaris by utilizing silkworm larvae
  • Method for breeding cordyceps militaris by utilizing silkworm larvae
  • Method for breeding cordyceps militaris by utilizing silkworm larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Selection of Cordyceps militaris strain host

[0032] Cordyceps militaris is cultivated with cooked silkworms (the 5th instar silkworm larvae develop to the end and stop eating mulberry leaves, which are called cooked silkworms) as hosts. The mature silkworms are required to have transparent bodies and light red feces.

[0033] (2) Preparation of Cordyceps militaris strain

[0034] Add a fruiting body of Cordyceps militaris (frozen preservation or fresh fruiting body) in a good growth state to the sterile PDA slant medium, cultivate it in a dark environment at 19°C, and carry out light culture after the aerial hyphae cover the entire slope , after the aerial mycelium turns yellow completely, gently scrape out the mycelium in a sterile environment, add sterile water and grind to make a suspension of Cordyceps militaris, the concentration of the suspension of Cordyceps militaris is 0.1mg / μL (based on the mass of fresh mycelium).

[0035] (3) Hypothermic anesthesia a...

Embodiment 2

[0046] (1) Selection of Cordyceps militaris strain host

[0047] Cordyceps militaris is cultivated with cooked silkworms (the 5th instar silkworm larvae develop to the end and stop eating mulberry leaves, which are called cooked silkworms) as hosts. The mature silkworms are required to have transparent bodies and light red feces.

[0048] (2) Preparation of Cordyceps militaris strain

[0049] Add a fruiting body of Cordyceps militaris (frozen preservation or fresh fruiting body) in a good growth state to the sterile PDA slant medium, cultivate it in a dark environment at 20°C, and carry out light culture after the aerial hyphae cover the entire slope , after the aerial mycelium turns yellow completely, gently scrape out the mycelium in a sterile environment, add sterile water and grind to make a suspension of Cordyceps militaris, the concentration of the suspension of Cordyceps militaris is 0.1mg / μL (Based on the mass of fresh mycelium).

[0050] (3) Hypothermic anesthesia a...

Embodiment 3

[0061] (1) Selection of Cordyceps militaris strain host

[0062] Cordyceps militaris is cultivated with cooked silkworms (the 5th instar silkworm larvae develop to the end and stop eating mulberry leaves, which are called cooked silkworms) as hosts. The mature silkworms are required to have transparent bodies and light red feces.

[0063] (2) Preparation of Cordyceps militaris strain

[0064] Add a fruiting body of Cordyceps militaris (frozen preservation or fresh fruiting body) in a good growth state to the sterile PDA slant medium, cultivate it in a dark environment at 20°C, and carry out light culture after the aerial hyphae cover the entire slope , after the aerial mycelium turns yellow completely, gently scrape out the mycelium in a sterile environment, add sterile water and grind to make a suspension of Cordyceps militaris, the concentration of the suspension of Cordyceps militaris is 0.1mg / μL (Based on the mass of fresh mycelium).

[0065] (3) Hypothermic anesthesia a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a novel method for breeding cordyceps militaris by utilizing silkworm larvae. The method comprises the steps of taking live matured silkworm as the host, vaccinating cordyceps militaris strains through an injection method, and promoting silkworm to sleep, and the vaccination attainment rate is improved; after the method is adopted, the inactivation rate of the silkworm larvae reaches 90% to 100%, and the outgrowth rate of fruiting body reaches 85% to 100%; moreover, the water-solubility protein, polysaccharide, cordycepic acid, cordycepin and adenosine of the cordyceps militaris bred by the method are higher than those of cordyceps militaris bred by silkworm chrysalis in content. The novel method for breeding cordyceps militaris by utilizing silkworm larvae has the advantages of convenience in operation, low breeding cost, good experiment repeatability and wide application prospect, and is suitable for large-scale cultivation.

Description

Technical field: [0001] The invention belongs to the field of edible fungus cultivation, and relates to a method for artificially cultivating Cordyceps militaris, in particular to a method for cultivating Cordyceps militaris with live silkworm larvae as hosts. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris or Cordyceps militaris, is a kind of edible and medicinal fungus recognized both at home and abroad. Studies have shown that the types and contents of effective chemical components of Cordyceps militaris are the same or similar to those of Cordyceps sinensis, and have the same or similar functions on the human body. They all contain biologically active substances such as cordycepic acid, cordycepin, polysaccharides, SOD enzymes and ergosterol, It has the effects of improving immunity, anti-tumor, anti-oxidation, anti-fatigue, anti-radiation, lowering blood sugar, lowering blood fat and delaying aging (Wei Huiping, 2004). [0003] Com...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01G1/04
Inventor 贾俊强桂仲争吴琼英
Owner 北京航天农业生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com