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Method for producing biological water-purifying agent through microbe mutual fermentation of chicken manure

A technology for fermenting chicken manure and water purifying agent is applied in the field of agricultural waste utilization, which can solve the problems of livestock and poultry manure pollution and increase urban pressure, and achieve the effects of improving water quality, high efficiency and high conversion rate.

Active Publication Date: 2013-03-20
长沙浩博生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most livestock and poultry farms do not treat manure, so the pollution of livestock and poultry manure is increasing the pressure on cities and towns

Method used

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  • Method for producing biological water-purifying agent through microbe mutual fermentation of chicken manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The effective microbial strains in the chicken manure fermentation process were Aspergillus oryzae [ACCC30155], Saccharomyces cerevisiae [ACCC20065], Bacillus subtilis [ACCC10148] and photosynthetic bacteria [ACCC10650], all of which were purchased from China Agricultural Microbiology Center.

[0015] Aspergillus oryzae and yeast medium: 10% wort medium, no agar is added as a liquid medium, and 2% agar is added as a solid medium.

[0016] Bacillus subtilis culture medium: nutrient broth, liquid medium without agar, solid medium with 2% agar.

[0017] Photosynthetic bacteria medium: PSB medium, CH 3 COONa 3.0g·L -1 , (NH 4 ) 2 SO 4 , 1.5g·L -1 , MgSO 4 ·7H 2 O 0.8g·L -1 , KH 2 PO 4 0.3g·L -1 , K 2 HPO 4 0.5g·L -1 , yeast extract powder 1.2g·L -1 , pH 7.5, no agar is added as a liquid medium, and 2% agar is added as a solid medium.

[0018] Inoculate the above-mentioned strains on the corresponding slopes for activation, and then inoculate them into the ...

Embodiment 2

[0026] The activation culture of the strain is the same as in Example 1.

[0027] 55wt% fresh chicken manure, 27% Pleurotus eryngii fungus chaff, 18% inorganic compound fertilizer solid raw material are mixed evenly, then inoculated with Aspergillus oryzae 6wt% accounting for solid raw material gross weight, Saccharomyces cerevisiae 2wt%, Bacillus subtilis 2wt% and Photosynthetic bacteria 2wt%, then the thickness of the material is kept at 50-60 cm, piled up at room temperature and fermented for 15 days, and stirred twice during the fermentation process to obtain the product of the present invention. The number of effective microorganisms after fermentation is about 4×10 8 CFU / g, according to different users, inorganic fertilizers can be added to make products with different nutrients.

[0028] Take 100 kilograms of the above-mentioned products, add 20 kilograms of inorganic compound fertilizers (products with a nutrient content of 17%N:5%P:8%K on the market) and mix them to ...

Embodiment 3

[0031] The activation culture of the strain is the same as in Example 1.

[0032] 55wt% fresh chicken manure, 27% Pleurotus eryngii fungus chaff, 18% inorganic compound fertilizer solid raw material are mixed evenly, then inoculated with Aspergillus oryzae 2wt% accounting for solid raw material gross weight, Saccharomyces cerevisiae 1wt%, Bacillus subtilis 1wt% and 1 wt% of photosynthetic bacteria, then the thickness of the material is kept at 50-60 centimeters, piled up and fermented at room temperature for 15 days, and stirred twice during the fermentation process to obtain the product of the present invention. The effective number of microorganisms after fermentation is 3×10 8 CFU / g, according to different users, inorganic fertilizers can be added to make products with different nutrients.

[0033] Take 100 kg of the above product, add 15 kg of inorganic compound fertilizer (products with a nutrient content of 17%N:5%P:8%K on the market are acceptable) and mix it to get a ...

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Abstract

The invention discloses a method for producing a biological water-purifying agent through microbe mutual fermentation of chicken manure. The method comprises: mixing 50-65% of fresh chicken manure, 20-30% of pleurotus eryngii residues, and 15-20% of an inorganic compound fertilizer, and inoculating Aspergillus oryzae, a yeast, a photosynthetic bacterium and bacillus subtilis into the mixture for fermentation, which can generate an effective microbe number of 3-4*10<8>CFU / g. Specifically, the inoculated strains are: 2-6 wt% of a solid strain of Aspergillus oryzae ACCC30155, 1-2 wt% of a liquid strain of saccharomyces cerevisiae ACC20065, 1-2 wt% of a liquid strain of bacillus subtilis ACCC10148, and 1-2 wt% of a liquid strain of photosynthetic bacterium ACCC10650. After 15-20d of stacking fermentation, the biological water-purifying agent can be obtained. And the produced biological water-purifying agent of the invention can be prepared into different water purifying agents and aquaculture products according to needs, thus boasting wide application prospects in agriculture, aquaculture industry and environmental protection industry.

Description

technical field [0001] The invention belongs to the technical field of agricultural waste utilization, in particular to producing a biological water purifying agent by transforming livestock and poultry manure through microbial fermentation, and then preparing a water body purifying agent or aquaculture product for water cultivation and fertilization. Background technique [0002] In recent years, large-scale, intensive, and modern farms have shown a sharp upward trend. Due to unsound environmental regulations and shortage of funds, the issue of manure disposal is often not considered at the beginning of farm construction. According to measurements, a factory chicken farm that raises 100,000 chickens can produce up to 10 tons of chicken manure per day, and an annual output of more than 3,600 tons of chicken manure. Most livestock and poultry farms do not treat the manure, so the pollution of livestock and poultry manure is increasing the pressure on cities and towns. The ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/18C12N1/20C02F3/34C05F17/00C12R1/01C12R1/125C12R1/69C12R1/865
CPCY02W30/40
Inventor 谭周进邓悟森丁祥力伍兵
Owner 长沙浩博生物技术有限公司
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