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Composite microbe oil displacement agent and use thereof

A technology of composite microorganisms and oil displacement agents, applied in the direction of microorganisms, microorganism-based methods, drilling compositions, etc., can solve the problems of poor oil displacement effect, high environmental pollution, high production cost, etc., and achieve easy large-scale production and application, low concentration and low production cost

Inactive Publication Date: 2012-03-28
李立文 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the physical and chemical means currently used can achieve the purpose of effective oil displacement, high energy consumption and high environmental pollution have become the bottleneck restricting the development of the petroleum industry.
In recent years, a lot of research has been done on the use of microbial oil displacement agents in the fields of oil displacement, plug removal, demulsification and environmental protection at home and abroad. However, due to factors such as high production costs and poor oil displacement effects, it has not been widely used. application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of Pseudomonas aeruginosa fermentation whole culture

[0027] Streak inoculate the frozen Pseudomonas aeruginosa on the LB plate containing 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl and 15g / L agar, and place it under the condition of 37℃ and saturated humidity Incubate overnight. Pick colonies cultured on LB plate, inoculate in LB medium containing 10g / L tryptone, 5g / L yeast extract and 10g / L NaCl, and culture overnight at 37°C and 220rpm. The overnight culture was inoculated in LB medium containing 5.0 g / L fish meal at a ratio of 1:10, and cultured for 12 h at 37°C with an air flow of 1.0 VVM to obtain Pseudomonas aeruginosa seed liquid. The seed solution was inoculated with urea 0.5g / L at a ratio of 1:10, K 2 HPO 4 2.0g / L, MgSO 4 .6H 2 O 5.0mg / L, FeCl 3 .6H 2 O 0.16mg / L, ZnSO 4 .7H 2 O 1.5mg / L, CuSO 4 .5H 2 O 0.15mg / L, MnSO 4 .4H 2 O 1.5mg / L, H 3 BO 3 0.03mg / L, vegetable oil 80mL / L, fishmeal 5.0g / L fermentation medium, ferme...

Embodiment 2

[0028] Example 2 Compounding of Pseudomonas aeruginosa fermentation whole culture and chemical surfactant

[0029] The non-ionic surfactant OP-10 was added to the whole fermentation culture of Pseudomonas aeruginosa in Example 1 to a final concentration of 6%, and stored at room temperature after sub-packaging for future use.

Embodiment 3

[0030] Example 3 Preparation of whole culture of Geobacillus fermentation

[0031] Streak inoculate frozen Geobacillus thermodenitrophilus on LB plate containing 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl and 15g / L agar, place at 60℃, saturated humidity overnight culture under conditions. Pick Geobacillus thermodenitrophilus cultured on LB plates, inoculate them in LB medium containing 10g / L tryptone, 5g / L yeast extract and 10g / L NaCl, and culture overnight at 60°C and 220rpm. The overnight culture of Geobacillus thermodenitrophilus was inoculated in LB medium at a ratio of 1:10, and cultured for 12 hours at 60°C with an air flow of 1.0VVM to obtain the seed liquid of Geobacillus thermodenitrophilus. Inoculate the seed solution of Geobacillus thermodenitrophilus in a 1:10 ratio containing sucrose 10.0g / L, starch 10.0g / L, NH 4 Cl 6.0g / L, MgSO 4 .7H 2 O 0.2g / L, KH 2 PO 4 4.0g / L, Na 2 HPO 4 12.0g / L, EDTA 0.14g / L, CaCl 2 0.08g / L, fishmeal 3.0g / L fermentation medium...

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PUM

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Abstract

The invention discloses a composite microbe oil displacement agent and the use thereof. The composite microbe oil displacement agent comprises pseudomonas fermentation anaculture and geobacillus fermentation anaculture, and the volume ratio of the pseudomonas fermentation anaculture to the geobacillus fermentation anaculture ranges from 1:10 to 10:1. The composite microbe oil displacement agent is non-toxic and harmless, is easy to degrade, and is an environmental-friendly biological agent; the manufacturing cost is low, and the composite microbe oil displacement agent is easy to manufacture and apply on a large scale; the using temperature range is wide (from 10 to 100 DEG C), and high-viscosity crude oil emulsion can be processed through emulsion breaking effectively; deposits cannot be generated after the composite microbe oil displacement agent is mixed with high hardness groundwater, and the composite microbe oil displacement agent is suitable for oil displacement, plugging removal and emulsion breaking of a low permeability oilfield and an ultra-low permeability oilfield; and the composite microbe oil displacement agent achieves high efficiency of oil displacement and emulsion breaking.

Description

technical field [0001] The invention relates to a composite microbial oil displacement agent, in particular to a composite microbial oil displacement agent containing a fermented whole culture of Pseudomonas aeruginosa and a fermented whole culture of Geobacillus thermodenitrophilus and its application in oil flooding and oil well decontamination. Plugging and demulsification of oil-in-water produced fluid and oil-in-water emulsion. Background technique [0002] Enhanced oil recovery is a key issue in the petroleum industry. Although the physical and chemical means currently used can achieve the purpose of effective oil displacement, high energy consumption and high environmental pollution have become the bottleneck restricting the development of the petroleum industry. In recent years, a lot of research has been done on the use of microbial oil displacement agents in the fields of oil displacement, plug removal, demulsification and environmental protection at home and abro...

Claims

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Application Information

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IPC IPC(8): C09K8/582C09K8/52C10G33/00C12N1/20C12R1/10C12R1/385
Inventor 李立文曹力元李锁良
Owner 李立文
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