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Method for extracting taxol simply, conveniently and efficiently

A high-efficiency technology for paclitaxel, applied in the production of bulk chemicals, organic chemistry, etc., can solve the problems of low extraction rate of paclitaxel, unsuitable for large-scale industrial production, etc., and achieve the effects of improving extraction and purification steps, reducing losses and reducing costs.

Active Publication Date: 2012-02-29
赵华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention provides a simple and efficient method for extracting paclitaxel to solve the problems of low extraction rate of paclitaxel and unsuitability for large-scale industrial production

Method used

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  • Method for extracting taxol simply, conveniently and efficiently

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Grind 1000g of dried yew medicinal materials into coarse powder, pass through a 20-mesh sieve, and use supercritical CO 2 Extraction method to remove fat-soluble impurities, extraction temperature: 40°C, extraction pressure: 25MPa, extraction time 3h, without entrainer;

[0020] (2) Add 8 g of cellulase to the medicinal dregs, add 8 L of water, and hydrolyze at 70° C. for 30 hours;

[0021] (3) filter, after the dregs of the medicine are dried, extract with 5 times amount of ethanol, the extraction method adopts the percolation method: put the above-mentioned medicine dregs into the percolation cylinder, use 95% ethanol as the solvent, first soak for 12 hours, and then Percolate starts to flow out from the lower port, and new solvent is continuously added to the upper port of the percolation cylinder until there is no more paclitaxel, and the solvent always maintains a concentration difference;

[0022] (4) Filtrate, recover ethanol under reduced pressure at 35°C u...

Embodiment 2

[0026] (1) Grind 1000g of dried yew medicinal materials into coarse powder, pass through a 20-mesh sieve, and use supercritical CO 2 Extraction method to remove fat-soluble impurities, extraction temperature: 50°C, extraction pressure: 30MPa, extraction time 2h, without entrainer;

[0027] (2) Add 10 g of cellulase to the dregs, add 10 L of water, and hydrolyze at 65°C for 25 hours;

[0028] (3) filter, after dregs are dried, extract with 8.5 times amount of ethanol, the extraction method adopts the percolation method: put the above dregs into the percolation cylinder, use 95% ethanol as solvent, soak for 30 hours at first, then by Percolate starts to flow out from the lower port, and new solvent is continuously added to the upper port of the percolation cylinder until there is no more paclitaxel, and the solvent always maintains a concentration difference;

[0029] (4) Filtrate, recover ethanol under reduced pressure at 47°C until the residue has no alcohol smell;

[0030] ...

Embodiment 3

[0033] (1) Grind 1000g of dried yew medicinal materials into coarse powder, pass through a 20-mesh sieve, and use supercritical CO 2 Extraction method to remove fat-soluble impurities, extraction temperature: 60°C, extraction pressure: 35MPa, extraction time 1h, without entrainer;

[0034] (2) Add 12 g of cellulase to the medicinal dregs, add 12 L of water, and hydrolyze at 60° C. for 20 hours;

[0035] (3) Filtration, after the medicinal residues are dried, extract with 12 times the amount of ethanol, percolation method: put the above medicinal residues into the percolation cylinder, use 95% ethanol as solvent, first soak for 48 hours, and then start to flow out from the lower mouth For the percolation solution, new solvents are continuously added to the top of the percolation cylinder until there is no more paclitaxel, and the concentration difference of the solvents is always maintained.

[0036] (4) Filtrate, recover ethanol under reduced pressure at 60°C until the residu...

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Abstract

The invention relates to a method for extracting taxol simply, conveniently and efficiently and belongs to a method for extracting taxol. The method comprises: crushing a taxus chinensis dry medicinal material into coarse powder, removing fat soluble impurities by a supercritical CO2 extraction process, and adding cellulase into medicinal residue for hydrolysis; and filtering, drying the medicinal residue, leaching in ethanol, filtering, recovering ethanol under reduced pressure till the residue gives off no alcohol smell, washing the residual with water, discarding washing solution, drying and obtaining taxol by chromatography. The method improves the extraction rate of taxol, uses a small volume of water, avoids using an organic solvent in a solvent extraction method, reduces column loading pressure and is more suitable for mass industrial production.

Description

technical field [0001] The invention belongs to the extraction method of paclitaxel. Background technique [0002] Taxus, scientific name Taxus chinensis (pilger) Rehd.; commonly known as yew, belongs to the plant kingdom, gymnosperm, Taxaceae (Taxaceae), Taxusaceae, Taxaceae, Taxus genus. Paclitaxel is a new substance with anti-cancer effect extracted from yew. The main component of paclitaxel is a diterpene core composed of three main rings and a phenylisoserine side chain, the molecular formula is C 47 h 51 NO 14 , the molecular weight is 853.92, and the structural formula is as follows: [0003] [0004] At present, there are roughly two types of patents on paclitaxel obtained from yew in my country: extraction and purification. The extraction of paclitaxel from Taxus is basically divided into five types: methanol extraction, ethanol extraction, cell culture, biological enzymatic hydrolysis, and supercritical carbon dioxide method; among them, ethanol extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
CPCY02P20/54
Inventor 赵华白雪媛王诗涵刘婉怡周晓玲梁钟艺
Owner 赵华
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